Use of regulated secretion in protein production from animal cells: An evaluation with the AtT-20 model cell line

1990 ◽  
Vol 35 (8) ◽  
pp. 771-780 ◽  
Author(s):  
A. Sambanis ◽  
Gregory Stephanopoulos ◽  
A. J. Sinskey ◽  
H. F. Lodish
Keyword(s):  
Cell Line ◽  
Protein Production ◽  
Animal Cells ◽  
Regulated Secretion ◽  
Model Cell

scholarly journals Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1183
Author(s):  
Cecilia Spedalieri ◽  
Gergo Péter Szekeres ◽  
Stephan Werner ◽  
Peter Guttmann ◽  
Janina Kneipp
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Early Stage ◽  
Protein Corona ◽  
Fibroblast Cell ◽  
Ultrastructural Level ◽  
Epithelial Cell Line ◽  
Animal Cells ◽  
Gold Nanostars

Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics.

scholarly journals Signalling mechanisms regulating phenotypic changes in breast cancer cells

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Natalia Volinsky ◽  
Cormac J. McCarthy ◽  
Alex von Kriegsheim ◽  
Nina Saban ◽  
Mariko Okada-Hatakeyama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Phenotypic Changes ◽  
Model Cell ◽  
Accumulation Of Lipids

Excessive production and accumulation of lipids is often observed in breast cancer tissue. In the current study, we investigate signalling mechanisms regulating this process using a model cell line.

scholarly journals Qu-2, a robust poplar suspension cell line for molecular biology

2020 ◽  
Author(s):  
Caixia Liu ◽  
Kailong Li ◽  
Meng Wang ◽  
Erqin Fan ◽  
Chuanping Yang ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Cell Line ◽  
Woody Plants ◽  
Culture Media ◽  
High Growth ◽  
Fold Increase ◽  
Suspension Cell ◽  
Biological Research ◽  
Model Cell ◽  
Suspension Cell Line

AbstractPopulus spp. have long been used as model woody plant species for molecular biology research. However, tissues of poplar are often recalcitrant to experimental procedures for molecular studies. We generated a hormone autotrophic poplar suspension cell line from a hybrid of Populus alba × P. berolinensis ‘Yinzhong’, named Qu-2. Qu-2 cells are suitable as a model biological system for studying woody plants. Qu-2 cells have many advantages over suspension cell lines derived so far from any other woody plants. Qu-2 cells are very easy to cultivate and can grow on several common plant culture media without the addition of any plant hormone. They show exceptionally high growth rates, reaching an approximately 150-fold increase in biomass after one week of culturing. Another important unique characteristic of Qu-2 cells is that they can be cryopreserved and readily reactivated. Qu-2 cells are suitable for molecular manipulations such as protoplast production, transient transformation, and RNA-seq analysis. Therefore, Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research, ranging from physiology to gene function. The Qu-2 cells will be made available to the plant community for research.

scholarly journals Sorting Protein-related Receptor SorLA Controls Regulated Secretion of Glial Cell Line-derived Neurotrophic Factor

2011 ◽  
Vol 286 (48) ◽  
pp. 41871-41882 ◽  
Author(s):  
Zhao Geng ◽  
Feng-Yi Xu ◽  
Shu-Hong Huang ◽  
Zhe-Yu Chen
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Regulated Secretion
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