Effects of temperature on cell growth and xanthan production in batch cultures ofXanthomonas campestris

1990 ◽  
Vol 35 (5) ◽  
pp. 454-468 ◽  
Author(s):  
Chin-Hang Shu ◽  
Shang-Tian Yang
Keyword(s):  
Cell Growth ◽  
Batch Cultures ◽  
Xanthan Production ◽  
Effects Of Temperature

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

Xanthan production by Xanthomonas campestris in batch cultures

2001 ◽  
Vol 37 (1) ◽  
pp. 73-80 ◽  
Author(s):  
M Papagianni ◽  
S.K Psomas ◽  
L Batsilas ◽  
S.V Paras ◽  
D.A Kyriakidis ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Batch Cultures ◽  
Xanthan Production

scholarly journals Growth, stoichiometry and cell size; temperature and nutrient responses in haptophytes

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3743 ◽  
Author(s):  
Lars Fredrik Skau ◽  
Tom Andersen ◽  
Jan-Erik Thrane ◽  
Dag Olav Hessen
Keyword(s):  
Growth Rate ◽  
High Temperature ◽  
Cell Size ◽  
Marine Phytoplankton ◽  
Effect Of Temperature ◽  
Elemental Content ◽  
Specific Content ◽  
Batch Cultures ◽  
P Limitation ◽  
Effects Of Temperature

Temperature and nutrients are key factors affecting the growth, cell size, and physiology of marine phytoplankton. In the ocean, temperature and nutrient availability often co-vary because temperature drives vertical stratification, which further controls nutrient upwelling. This makes it difficult to disentangle the effects of temperature and nutrients on phytoplankton purely from observational studies. In this study, we carried out a factorial experiment crossing two temperatures (13°and 19°C) with two growth regimes (P-limited, semi-continuous batch cultures [“−P”] and nutrient replete batch cultures in turbidostat mode [“+P”]) for three species of common marine haptophytes (Emiliania huxleyi, Chrysochromulina rotalis and Prymnesium polylepis) to address the effects of temperature and nutrient limitation on elemental content and stoichiometry (C:N:P), total RNA, cell size, and growth rate. We found that the main gradient in elemental content and RNA largely was related to nutrient regime and the resulting differences in growth rate and degree of P-limitation, and observed reduced cell volume-specific content of P and RNA (but also N and C in most cases) and higher N:P and C:P in the slow growing −P cultures compared to the fast growing +P cultures. P-limited cells also tended to be larger than nutrient replete cells. Contrary to other recent studies, we found lower N:P and C:P ratios at high temperature. Overall, elemental content and RNA increased with temperature, especially in the nutrient replete cultures. Notably, however, temperature had a weaker–and in some cases a negative–effect on elemental content and RNA under P-limitation. This interaction indicates that the effect of temperature on cellular composition may differ between nutrient replete and nutrient limited conditions, where cellular uptake and storage of excess nutrients may overshadow changes in resource allocation among the non-storage fractions of biomass (e.g. P-rich ribosomes and N-rich proteins). Cell size decreased at high temperature, which is in accordance with general observations.

Effects of temperature and nutritional conditions on the mitotic cell cycle of Saccharomyces cerevisiae

1978 ◽  
Vol 31 (1) ◽  
pp. 71-78
Author(s):  
M.N. Jagadish ◽  
B.L. Carter
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Growth Rates ◽  
Mitotic Cell ◽  
S Phase ◽  
Yeast Cells ◽  
Batch Cultures ◽  
Nutritional Conditions ◽  
Limiting Nutrient ◽  
Effects Of Temperature

Yeast cells were cultivated at different growth rates in a chemostat by alterations in the flow of the limiting nutrient glucose and in batch cultures where variations in growth rate were achieved by alterations in the composition of nutrients. It was observed that the stage in the cycle at which S-phase was completed varied with growth rate. The faster the growth rate, the earlier the stage in the cycle in which completion of S-phase occurred. When stage in the cycle is converted into time before division it was observed that the time from completion of S-phase to cell division varied only slightly with growth rate except at extremely slow growth rates. Expansion of cell cycle transit time as the growth rate was slowed was achieved primarily by an expansion in time of the period from division to the completion of S-phase. In contrast, when cells were grown at different rates by alterations in the temperature of cultivation, completion of S-phase occurred at approximately the same stage in the cell cycle at all growth rates.

scholarly journals Bacterial Reductive Dissolution of Crystalline Fe(III) Oxide in Continuous-Flow Column Reactors

2000 ◽  
Vol 66 (3) ◽  
pp. 1062-1065 ◽  
Author(s):  
Eric E. Roden ◽  
Matilde M. Urrutia ◽  
Carroll J. Mann
Keyword(s):  
Cell Growth ◽  
Continuous Flow ◽  
Reductive Dissolution ◽  
Oxide Content ◽  
Inorganic Contaminants ◽  
Oxide Reduction ◽  
Batch Cultures ◽  
Reduction Activity ◽  
Coated Sand ◽  
Phase Transport

ABSTRACT Bacterial reductive dissolution of synthetic crystalline Fe(III) oxide-coated sand was studied in continuous-flow column reactors in comparison with parallel batch cultures. The cumulative amount of aqueous Fe(II) exported from the columns over a 6-month incubation period corresponded to (95.0 ± 3.7)% (n = 3) of their original Fe(III) content. Wet-chemical analysis revealed that only (6.5 ± 3.2)% of the initial Fe(III) content remained in the columns at the end of the experiment. The near-quantitative removal of Fe was visibly evidenced by extensive bleaching of color from the sand in the columns. In contrast to the column reactors, Fe(II) production quickly reached an asymptote in batch cultures, and only (13.0 ± 2.2)% (n = 3) of the Fe(III) oxide content was reduced. Sustained bacterial-cell growth occurred in the column reactors, leading to the production and export of a quantity of cells 100-fold greater than that added during inoculation. Indirect estimates of cell growth, based on the quantity of Fe(III) reduced, suggest that only an approximate doubling of initial cell abundance was likely to have occurred in the batch cultures. Our results indicate that removal of biogenic Fe(II) via aqueous-phase transport in the column reactors decreased the passivating influence of surface-bound Fe(II) on oxide reduction activity, thereby allowing a dramatic increase in the extent of Fe(III) oxide reduction and associated bacterial growth. These findings have important implications for understanding the fate of organic and inorganic contaminants whose geochemical behavior is linked to Fe(III) oxide reduction.

Carotenoid Production by Phaffia rhodozyma Using Raw Glycerol as an Additional Carbon Source

2012 ◽  
Vol 8 (4) ◽  
Author(s):  
Carolina M. Silva ◽  
Thais M. Borba ◽  
Carlos A V. Burkert ◽  
Janaína F M. Burkert
Keyword(s):  
Culture Medium ◽  
Cell Concentration ◽  
Carbon Sources ◽  
Phaffia Rhodozyma ◽  
Glycerol Concentration ◽  
Raw Glycerol ◽  
Batch Cultures ◽  
Pure Glycerol ◽  
Significant Difference ◽  
Effects Of Temperature

Abstract This paper presents pure glycerol and raw glycerol as additional carbon sources in the production of carotenoids by Phaffia rhodozyma NRRL Y-17268. Batch cultures in shaken flasks were performed in order to verify the effects of temperature cultivation (20 and 25ºC) and pure glycerol concentration (0, 10 and 40 g.L-1). The increase from 20 to 25ºC led to a significant increase (p<0.05) in cell concentration for all the pure glycerol concentrations, reaching the maximum levels of 8.9 g.L-1 of biomass (168 h), specific production of carotenoids (SPC) of 91.0 μg.g-1 (72 h) and volumetric production of carotenoids (VPC) of 2.0 μg.mL-1 (168 h) with 40 g.L-1 of pure glycerol at 25ºC. The use of 40 g.L-1 of raw glycerol at the same temperature resulted in a carotenogenic production showing no significant difference (p>0.05) in pure glycerol, reaching the maximum values of 8.3 g.L-1 of biomass (168 h) and within 120 h SPC 275.5 μg.g-1 and VPC 1.7 μg.mL-1. Therefore, the pure glycerol and raw glycerol can be used as additional carbon sources in the culture medium of P. rhodozyma.

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