Characteristics of immobilized lipase-catalyzed hydrolysis of olive oil of high concentration in reverse phase system

1989 ◽  
Vol 33 (11) ◽  
pp. 1469-1476 ◽  
Author(s):  
Sung Tae Kang ◽  
Joon Snick Rhee
Keyword(s):  
Olive Oil ◽  
Immobilized Lipase ◽  
Phase System ◽  
High Concentration ◽  
Reverse Phase ◽  
Hydrolysis Of

High Pressure Liquid Chromatographic Separation and Identification of Estrogens

1980 ◽  
Vol 63 (1) ◽  
pp. 80-87
Author(s):  
Robert W Roos
Keyword(s):  
High Pressure ◽  
Phase System ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Conjugated Estrogens ◽  
Phase Partition ◽  
Low Concentrations ◽  
Liquid Chromatographic Separation ◽  
Liquid Chromatographic ◽  
Hydrolysis Of

Abstract The use of high pressure liquid chromatography for separating mixtures of various estrogens is described. Separations of as many as 9 estrogens were studied using normal and reverse phase partition systems; the reverse phase system was the most useful. The estrogens were detected at 254 nm, a wavelength that favors their detection at low concentrations. The results of the separation studies were applied successfully to the separation and identification of estrogens in aqueous suspensions of estrogenic substances and to conjugated estrogens in tablets, following hydrolysis of the conjugates. Most estrogen peaks were identified using chromatographic mobilities; in some cases chemical reaction with the estrogen was used to form derivatives to aid identification. In one instance, the ultraviolet-absorbing properties of the estrogen were compared with literature data in order to assign an identity.

Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate

2018 ◽  
Vol 69 (10) ◽  
pp. 2794-2798
Author(s):  
Alina Diana Panainte ◽  
Ionela Daniela Morariu ◽  
Nela Bibire ◽  
Madalina Vieriu ◽  
Gladiola Tantaru ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Retention Time ◽  
Chromatographic Separation ◽  
Bovine Hemoglobin ◽  
Reverse Phase ◽  
Hplc System ◽  
Fast Flow ◽  
Hydrolysis Of ◽  
Phase Column

A peptidic hydrolysate has been obtained through hydrolysis of bovine hemoglobin using pepsin. The fractioning of the hydrolysate was performed on a column packed with CM-Sepharose Fast Flow. The hydrolysate and each fraction was filtered and then injected into a HPLC system equipped with a Vydak C4 reverse phase column (0.46 x 25 cm), suitable for the chromatographic separation of large peptides with 20 to 30 amino acids. The detection was done using mass spectrometry, and the retention time, size and distribution of the peptides were determined.

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