A functionally integrated thermoplastic microdevice for one-step solid-phase-based nucleic acid purification and isothermal amplification for facile detection of foodborne pathogen

2016 ◽  
Vol 113 (12) ◽  
pp. 2614-2623 ◽  
Author(s):  
Minh Luan Ha ◽  
Yu Zhang ◽  
Nae Yoon Lee
Keyword(s):  
Nucleic Acid ◽  
Solid Phase ◽  
Foodborne Pathogen ◽  
Isothermal Amplification ◽  
One Step ◽  
Nucleic Acid Purification

One-step purification and concentration of DNA in porous membranes for point-of-care applications

Lab on a Chip ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 2647-2659 ◽  
Author(s):  
S. A. Byrnes ◽  
J. D. Bishop ◽  
L. Lafleur ◽  
J. R. Buser ◽  
B. Lutz ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Porous Membranes ◽  
Complex Samples ◽  
One Step ◽  
Single User ◽  
Nucleic Acid Purification

Nucleic acid purification in porous membranes at the point-of-care from complex samples including nasal matrix and blood using a single-user step.

scholarly journals Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification

2016 ◽  
Vol 9 ◽  
pp. IDRT.S32162 ◽  
Author(s):  
Sayli S. Modak ◽  
Cheryl A. Barber ◽  
Eran Geva ◽  
William R. Abrams ◽  
Daniel Malamud ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Isothermal Amplification ◽  
Blood Smears ◽  
Circulating Antigen ◽  
Amplification Assay ◽  
Amplification Loop ◽  
Nucleic Acid Purification ◽  
Antigen Tests ◽  
Mitochondrial Cytochrome Oxidase

Malaria remains one of the most prevalent infectious diseases and results in significant mortality. Isothermal amplification (loop-mediated isothermal amplification) is used to detect malarial DNA at levels of ~1 parasite/μL blood in ≥30 minutes without the isolation of parasite nucleic acid from subject's blood or saliva. The technique targets the mitochondrial cytochrome oxidase subunit 1 gene and is capable of distinguishing Plasmodium falciparum from Plasmodium vivax. Malarial diagnosis by the gold standard microscopic examination of blood smears is generally carried out only after moderate-to-severe symptoms appear. Rapid diagnostic antigen tests are available but generally require infection levels in the range of 200–2,000 parasites/μL for a positive diagnosis and cannot distinguish if the disease has been cleared due to the persistence of circulating antigen. This study describes a rapid and simple molecular assay to detect malarial genes directly from whole blood or saliva without DNA isolation.

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