Enzyme immobilization in porous solid supports?penetration of immobilized enzyme

1984 ◽  
Vol 26 (9) ◽  
pp. 1032-1037 ◽  
Author(s):  
D. D. Do
Keyword(s):  
Enzyme Immobilization ◽  
Immobilized Enzyme ◽  
Porous Solid ◽  
Solid Supports

Modeling enzyme immobilization in porous solid supports

1982 ◽  
Vol 24 (7) ◽  
pp. 1527-1546 ◽  
Author(s):  
Duong D. Do ◽  
Douglas S. Clark ◽  
James E. Bailey
Keyword(s):  
Enzyme Immobilization ◽  
Porous Solid ◽  
Solid Supports

scholarly journals Multiscale investigations of europium(iii) complexation with tetra-n-octyl diglycolamide confined in porous solid supports

CrystEngComm ◽  
2020 ◽  
Vol 22 (41) ◽  
pp. 6886-6899
Author(s):  
Erin R. Bertelsen ◽  
Nolan C. Kovach ◽  
Benjamin J. Reinhart ◽  
Brian G. Trewyn ◽  
Mark R. Antonio ◽  
...  
Keyword(s):  
Porous Solid ◽  
Mesoporous Carbons ◽  
Loading Conditions ◽  
Solid Supports ◽  
Ordered Mesoporous Carbons ◽  
Ordered Mesoporous

Functionalized ordered mesoporous carbons facilitate the templateing of microcrystalline-like domains and multinuclear speciation under high Eu3+ loading conditions.

scholarly journals Peptide-Mediated Immobilization on Magnetoferritin for Enzyme Recycling

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1558 ◽  
Author(s):  
Zhang ◽  
Dong ◽  
Zhou ◽  
Hu ◽  
Li ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Immobilized Enzyme ◽  
Coiled Coil ◽  
Retention Rates ◽  
Generate Functional ◽  
Retention Efficiency ◽  
Enzyme Recycling ◽  
Lysine Residues ◽  
Target Molecules ◽  
Good Retention

Ferritin possess favorable properties because its exterior and interior surface can be applied to generate functional nanomaterials, which make them possible for enzyme immobilization and recycling. Here, we report the noncovalent immobilization of a genetically modified β-glucosidase onto the outer surface of synthetic magnetoferritin through the electrostatic interaction of a heterodimeric coiled-coil protein formed by coils containing lysine residues (K-coils) and coils containing glutamic acid (E-coils). The immobilized enzyme was characterized, and its enzymatic properties were evaluated. Furthermore, reusability of immobilized enzyme was demonstrated in aqueous solution under an applied magnetic field. The results showed that magnetoferritin was successfully prepared and it was an excellent support for enzyme immobilization. After three times usages, the retention rates were 93.75%, 82.5%, and 56.25%, respectively, demonstrating that immobilized enzyme possessed good retention efficiency and could be used as potential carrier for other biomolecules. The strategy of enzyme immobilization developed in this work can be applied, in general, to many other target molecules.

Physical and chemical immobilization of choline oxidase onto different porous solid supports: Adsorption studies

2016 ◽  
Vol 90 ◽  
pp. 76-82 ◽  
Author(s):  
Marieta L.C. Passos ◽  
David S.M. Ribeiro ◽  
João L.M. Santos ◽  
M.Lúcia M.F.S. Saraiva
Keyword(s):  
Porous Solid ◽  
Choline Oxidase ◽  
Solid Supports ◽  
Chemical Immobilization ◽  
Adsorption Studies ◽  
Physical And Chemical

EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME

2018 ◽  
Vol 80 (3) ◽  
Author(s):  
Suhaily Suhaimi ◽  
Rohaida Che Man ◽  
Natassha Jamil ◽  
Zatul Iffah Mohd Arshad ◽  
Shalyda Md Shaarani ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Hollow Fiber ◽  
Immobilized Enzyme ◽  
Hollow Fiber Membrane ◽  
Potato Starch ◽  
Enzymatic Reaction ◽  
Cyclodextrin Glucanotransferase ◽  
Fiber Membrane ◽  
Reaction Conditions ◽  
Agricultural Industries

The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.

scholarly journals Rapid protein immobilization for thin film continuous flow biocatalysis

2016 ◽  
Vol 52 (66) ◽  
pp. 10159-10162 ◽  
Author(s):  
Joshua Britton ◽  
Colin L. Raston ◽  
Gregory A. Weiss
Keyword(s):  
Thin Film ◽  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Continuous Flow ◽  
Immobilized Enzyme ◽  
Protein Immobilization

Continuous flow biocatalysis gets a new spin. An efficient and general enzyme immobilization technique for vortex fluidic processing has been developed. The immobilized enzyme demonstrated no decrease in enzyme activity over 10 h in continuous flow with a >95% reduction in quantities of required reagents and enzymes.

Confinement Phenomenon Effect on the CO2 Absorption Working Capacity in Ionic Liquids Immobilized into Porous Solid Supports

Langmuir ◽  
2017 ◽  
Vol 33 (42) ◽  
pp. 11719-11726 ◽  
Author(s):  
Nanhua Wu ◽  
Xiaoyan Ji ◽  
Wenlong Xie ◽  
Chang Liu ◽  
Xin Feng ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Porous Solid ◽  
Co2 Absorption ◽  
Working Capacity ◽  
Solid Supports
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