Development of a hollow-fiber system for large-scale culture of mammalian cells

1981 ◽  
Vol 23 (1) ◽  
pp. 79-95 ◽  
Author(s):  
K. Ku ◽  
M. J. Kuo ◽  
J. Delente ◽  
B. S. Wildi ◽  
J. Feder
Keyword(s):  
Hollow Fiber ◽  
Mammalian Cells ◽  
Large Scale ◽  
Fiber System ◽  
Large Scale Culture

Use of a hollow fiber bioreactor for large-scale production of α2-adrenoceptors in mammalian cells

1994 ◽  
Vol 37 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Sari Ala-Uotila ◽  
Anne Marjamäki ◽  
Marja-Terttu Matikainen ◽  
Markku Jalkanen
Keyword(s):  
Hollow Fiber ◽  
Mammalian Cells ◽  
Large Scale ◽  
Scale Production ◽  
Α2 Adrenoceptors ◽  
Hollow Fiber Bioreactor ◽  
Large Scale Production

Large-Scale Culture of Hybridoma and Mammalian Cells in Fluidized Bed Bioreactors

1987 ◽  
Vol 506 (1 Biochemical E) ◽  
pp. 129-146 ◽  
Author(s):  
ROBERT C. DEAN ◽  
SABHASH B. KARKARE ◽  
NITYA G. RAY ◽  
PETER W. RUNSTADLER ◽  
K. VENKATASUBRAMANIAN
Keyword(s):  
Fluidized Bed ◽  
Mammalian Cells ◽  
Large Scale ◽  
Large Scale Culture

Fully-automated roller bottle handling system for large scale culture of mammalian cells

1997 ◽  
Vol 52 (3) ◽  
pp. 289-294 ◽  
Author(s):  
Ryoichi Kunitake ◽  
Akira Suzuki ◽  
Hajime Ichihashi ◽  
Shoichi Matsuda ◽  
Osamu Hirai ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Roller Bottle ◽  
Handling System ◽  
Large Scale Culture

scholarly journals Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor

2021 ◽  
Author(s):  
Aniel Moya-Torres ◽  
Monika Gupta ◽  
Fabian Heide ◽  
Natalie Krahn ◽  
Scott Legare ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hollow Fiber ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
Continuous Production ◽  
Extracellular Matrix Protein ◽  
Close Monitoring ◽  
High Quality ◽  
Biolayer Interferometry ◽  
Hollow Fiber Bioreactor

Abstract The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affecting cell differentiation, proliferation, metabolism, and survival. We were able to produce large glycoprotein quantities in mammalian cells, which were engineered for protein overexpression and secretion into the media using the controlled environment provided by a hollow fiber bioreactor. Close monitoring of the internal bioreactor conditions allowed for stable production over an extended period of time. In addition to this, Netrin-1 concentrations were monitored in expression media through biolayer interferometry which allowed us to increase Netrin-1 media concentrations tenfold over our current flask systems while preserving excellent protein quality and in solution behavior. Our particular combination of genetic engineering, cell culture system, protein purification, and biophysical characterization permitted us to establish an efficient and continuous production of high-quality protein suitable for structural biology studies that can be translated to various biological systems. Key points • Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 • Biolayer interferometry allows target protein quantitation in expression media • High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality Graphical abstract

scholarly journals Automation of large scale transient protein expression in mammalian cells

2011 ◽  
Vol 175 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Yuguang Zhao ◽  
Benjamin Bishop ◽  
Jordan E. Clay ◽  
Weixian Lu ◽  
Margaret Jones ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mammalian Cells ◽  
Large Scale ◽  
Transient Protein Expression ◽  
Expression In Mammalian Cells
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