Batch‐ and continuous‐culture studies of a methane‐utilizing mixed culture

1980 ◽  
Vol 22 (10) ◽  
pp. 2097-2118 ◽  
Author(s):  
S. C. Lamb ◽  
J. C. Garver
Keyword(s):  
Continuous Culture ◽  
Mixed Culture ◽  
Culture Studies

Studies in intermicrobial symbiosis. Saccharomyces cerevisiae and Lactobacillus casei

1972 ◽  
Vol 18 (11) ◽  
pp. 1733-1742 ◽  
Author(s):  
R. D. Megee III ◽  
J. F. Drake ◽  
A. G. Fredrickson ◽  
H. M. Tsuchiya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Continuous Culture ◽  
Mixed Culture ◽  
Growth Medium ◽  
Lactobacillus Casei ◽  
Mixed Cultures ◽  
Genetic Characteristics ◽  
Culture Techniques ◽  
Pure Cultures

Saccharomyces cerevisiae and a riboflavin assay strain of Lactobacillus casei have been propagated anaerobically in mixed culture. Both batch and continuous culture techniques were used. By varying the concentrations of glucose and riboflavin in the growth medium, it was possible to produce symbioses of commensalism + competition, competition, and mutualism + competition. In short, the interaction prevailing is determined by the medium as well as by the genetic characteristics of the organisms. The behavior of the mixed cultures in these situations was predicted from data taken on pure cultures of the organisms.

Lactobacilli Isolated from Chicken Intestines: Potential Use as Probiotics

1999 ◽  
Vol 62 (3) ◽  
pp. 252-256 ◽  
Author(s):  
C. GUSILS ◽  
A. PÉREZ CHAIA ◽  
S. GONZÁLEZ ◽  
G. OLIVER
Keyword(s):  
Cell Surface ◽  
Mixed Culture ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Probiotic Properties ◽  
Culture Studies ◽  
Salmonella Gallinarum ◽  
Feed Formulation ◽  
Lactobacillus Animalis

Lactobacillus strains were tested for their in vitro probiotic properties. Cell surface hydrophobicity was found to be very high for Lactobacillus fermentum subsp. cellobiosus and Salmonella Gallinarum; high values could indicate a greater ability to adhere to epithelial cells. Studies on Lactobacillus animalis indicated relative cell surface hydrophobicities smaller than those of L. fermentum subsp. cellobiosus and L. fermentum. L. animalis and Enterococcus faecalis were able to coaggregate with L. fermentum subsp. cellobiosus and L. fermentum, respectively, but not with Salmonella Gallinarum. After mixed-culture studies for determining suitable growth behavior, the pair of strains L. animalis plus L. fermentum subsp. cellobiosus was selected for an attempted challenge against Salmonella Gallinarum. Double and triple mixed-culture studies indicated that selected lactobacillus strains were able to retain their beneficial characteristics in the presence of Salmonella Gallinarum such as presence of lectins, production of antimicrobial compounds, and ability to grow and compete. The selected microorganisms can be considered as potential ingredients for a chicken probiotic feed formulation intended to control salmonellosis and also improve poultry sanitation.

Continuous culture studies of xylose-fermentingZymomonas mobilis

1998 ◽  
Vol 70-72 (1) ◽  
pp. 353-367 ◽  
Author(s):  
Hugh G. Lawford ◽  
Joyce D. Rousseau ◽  
Ali Mohagheghi ◽  
James D. McMillan
Keyword(s):  
Continuous Culture ◽  
Culture Studies

CONTINUOUS CULTURE STUDIES ON FOUR SPECIES OF FIBROLYTIC RUMEN BACTERIA WITH PURE CELLULOSE AS SUBSTRATE

1984 ◽  
Vol 64 (5) ◽  
pp. 49-49
Author(s):  
A. KISTNER ◽  
J. H. KORNELIUS ◽  
G. S. MILLER
Keyword(s):  
Continuous Culture ◽  
Growth Rates ◽  
Buffer Capacity ◽  
Specific Growth ◽  
Ph Value ◽  
Culture Studies ◽  
Pure Cellulose ◽  
Constant Ph ◽  
Continuous Culture System ◽  
Specific Growth Rates

The specific growth rates of one strain each of Ruminoccoccus flavefaciens, R. albus, Bacteroides succinogenes and Clostridium polysaccharolyticum were measured at a constant pH value in a pH-auxostat continuous culture system, using ball-milled filter paper cellulose as limiting substrate. By manipulating the buffer capacity of the medium, the degree to which the substrate was utilized before fresh medium was dosed to the cultures was controlled. With all four species, the specific growth rates declines as the cultures were forced to utilize the more refractory (crystalline?) portion of the substrate, but the species differed in the steepness of this decline. (Graphs of specific growth rates versus degree of solubilization of cellulose will be presented.) These dissimilarities in behavior are probably a reflection of differences between the species in the complements of cellulases at their disposal. Key words: Continuous culture, cellulases, bacteria, cellulose

Mixed culture studies with the chemostat

1972 ◽  
Vol 22 (1) ◽  
pp. 105-123 ◽  
Author(s):  
H. Veldkamp ◽  
Holger W. Jannasch
Keyword(s):  
Mixed Culture ◽  
Culture Studies

A mixed bacterial population in a continuous culture with and without kaolinite

1975 ◽  
Vol 21 (2) ◽  
pp. 173-180 ◽  
Author(s):  
R. Z. Maigetter ◽  
R. M. Pfister
Keyword(s):  
Continuous Culture ◽  
Bacterial Population ◽  
Fluorescent Antibody ◽  
Microscopic Analysis ◽  
Growth Conditions ◽  
Fluorescent Antibody Technique ◽  
Culture Vessel ◽  
Pseudomonas Sp ◽  
Culture Studies ◽  
Antibody Technique

Chromobacterium lividum and a Pseudomonas sp. were grown in pure and mixed continuous culture with and without the clay-mineral, kaolinite. Irrespective of the growth conditions, C. lividum adhered to the wall of the culture vessel whereas the Pseudomonas sp. showed no such tendency, at least visually. During mixed culture studies, the organism which was initially established in the culture dominated. The ratio between C. lividum and the Pseudomonas sp. was about 20:1 when C. lividum was first established and 1:2 when the Pseudomonas sp. was first grown. The indirect fluorescent antibody technique provided a rapid method for differentiating the mixed cultures when the bacterial concentration was sufficient for microscopic analysis. During both pure and mixed continuous culture studies, the addition of kaolinite reduced the C. lividum but not the Pseudomonas sp. population.

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