scholarly journals Determination of the time to harvest foot-and-mouth disease virus cultures by measurements of the supernatant, concentration of lactic dehydrogenase

1977 ◽  
Vol 19 (6) ◽  
pp. 929-932 ◽  
Author(s):  
R. E. Spier
2008 ◽  
Vol 32 (2) ◽  
pp. 181-203
Author(s):  
Zaid Salah Hussein

For the determination of FMD infection in Nainawa province Serumsamples were collected from 92 suspected cases (42sheep,50cattle)thiswas performed in September 2007 and included nine region(Talafar,Basheca, Al-shamal, Hamamalaleel ,Cokajlly ,Zamaar ,Al-Namrood,AL-Koosh and Hemedaat), these samples were send frozen to the labs ofstate Vet. Company in Al-Nahdaa , the special test FMD-3ABC bo Ovenzyme immune assay on sheep serums showed that 30.95% werepositive to the test and 19.05% were ambiguous while the test on bovineserums showed increase in both positive and ambiguous results(42%,26% respectively), 8 serums that showed the highest positive resultto the former test were submitted to the special immunological test FMDElisa Kit Liquid Phase Blocking Immunoassay for detection of antibodiesof foot and mouth disease virus test which revealed that all sores of thesamples were infected with O serotype FMD


2014 ◽  
Vol 30 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Yiyang DONG ◽  
Yan XU ◽  
Zaixin LIU ◽  
Yuanfang FU ◽  
Toshinori OHASHI ◽  
...  

1994 ◽  
Vol 68 (9) ◽  
pp. 5677-5684 ◽  
Author(s):  
R Kirchweger ◽  
E Ziegler ◽  
B J Lamphear ◽  
D Waters ◽  
H D Liebig ◽  
...  

Author(s):  
S. S. Breese ◽  
H. L. Bachrach

Models for the structure of foot-and-mouth disease virus (FMDV) have been proposed from chemical and physical measurements (Brown, et al., 1970; Talbot and Brown, 1972; Strohmaier and Adam, 1976) and from rotational image-enhancement electron microscopy (Breese, et al., 1965). In this report we examine the surface structure of FMDV particles by high resolution electron microscopy and compare it with that of particles in which the outermost capsid protein VP3 (ca. 30, 000 daltons) has been split into smaller segments, two of which VP3a and VP3b have molecular weights of about 15, 000 daltons (Bachrach, et al., 1975).Highly purified and concentrated type A12, strain 119 FMDV (5 mg/ml) was prepared as previously described (Bachrach, et al., 1964) and stored at 4°C in 0. 2 M KC1-0. 5 M potassium phosphate buffer at pH 7. 5. For electron microscopy, 1. 0 ml samples of purified virus and trypsin-treated virus were dialyzed at 4°C against 0. 2 M NH4OAC at pH 7. 3, deposited onto carbonized formvar-coated copper screens and stained with phosphotungstic acid, pH 7. 3.


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