Immobilization of protein and polysaccharide on magnetic particles: Selective binding of microorganisms by concanavalin A-magnetite

1976 ◽  
Vol 18 (11) ◽  
pp. 1647-1651 ◽  
Author(s):  
M. Horisberger
Keyword(s):  
Concanavalin A ◽  
Magnetic Particles ◽  
Selective Binding

scholarly journals Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259846
Author(s):  
Yasuhiro Fujiwara ◽  
Yuji Tanno ◽  
Hiroki Sugishita ◽  
Yusuke Kishi ◽  
Yoshinori Makino ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Concanavalin A ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Protein A ◽  
Con A ◽  
Established Method ◽  
Genomic Location ◽  
Tn5 Transposase ◽  
Selection Of

Epigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription factors and histone modifications. A recently established method called CUT&Tag uses a Protein-A Tn5 transposase fusion protein, which cuts the genome and inserts adapter sequences nearby the target protein. Throughout most of the CUT&Tag procedure, cells are held on concanavalin A (con A)-conjugated magnetic beads. Proper holding of cells would be decisive for the accessibility of Tn5 to the chromatin, and efficacy of the procedure of washing cells. However, BioMag®Plus ConA magnetic beads, used in the original CUT&Tag protocol, often exhibit poor suspendability and severe aggregation. Here, we compared the BioMag beads and Dynabeads® magnetic particles of which conjugation of con A was done by our hands, and examined the performance of these magnetic beads in CUT&Tag. Among tested, one of the Dynabeads, MyOne-T1, kept excessive suspendability in a buffer even after overnight incubation. Furthermore, the MyOne-T1 beads notably improved the sensitivity in CUT&Tag assay for H3K4me3. In conclusion, the arrangement and the selection of MyOne-T1 refine the suspendability of beads, which improves the association of chromatin with Tn5, which enhances the sensitivity in CUT&Tag assay.

Synthesis of a Novel Polyaniline Glycopolymer and its Lectin Binding Studies

2014 ◽  
Vol 67 (4) ◽  
pp. 562 ◽  
Author(s):  
Christopher Wilcox ◽  
Jianyong Jin ◽  
Hayley Charville ◽  
Simon Swift ◽  
Teresa To ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Concanavalin A ◽  
Lectin Binding ◽  
Water Soluble ◽  
Binding Studies ◽  
Aniline Derivative ◽  
Selective Binding ◽  
Multistep Synthesis ◽  
Potential Applications ◽  
Mannose Receptors

We report the multistep synthesis and polymerisation of a novel aniline derivative with a pendant α-d-mannose substituent. The α-D-mannose functionality was successfully introduced before polymerisation via copper-catalysed azide alkyne click chemistry and the resulting monomer was polymerised using general oxidative polymerisation conditions, producing a water soluble mannosylated polyaniline. The polymer was characterised by several techniques and compared with standard polyaniline. The selective binding of the polymer to Concanavalin A (ConA) was successfully demonstrated by the precipitation of polymer–ConA aggregates. Potential applications of these novel polyaniline glycopolymers could include the development of electroactive biomaterials with the ability to bind mannose receptors, or as sensors for proteins or microbes.

Selective Binding of RNase B Glycoforms by Polydopamine-Immobilized Concanavalin A

2009 ◽  
Vol 81 (13) ◽  
pp. 5413-5420 ◽  
Author(s):  
Todd A. Morris ◽  
Alexander W. Peterson ◽  
Michael J. Tarlov
Keyword(s):  
Concanavalin A ◽  
Selective Binding ◽  
Rnase B

scholarly journals Preparation of "stress-free" concanavalin A-conjugated Dynabeads magnetic beads for CUT&Tag

2021 ◽  
Author(s):  
Yasuhiro Fujiwara ◽  
Yuji Tanno ◽  
Hiroki Sugishita ◽  
Yusuke Kishi ◽  
Yoshinori Makino ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Concanavalin A ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Protein A ◽  
Con A ◽  
Established Method ◽  
Genomic Location ◽  
Tn5 Transposase ◽  
Selection Of

Epigenome research has employed various methods to identify genomic location of proteins of interest, such as transcription factors and histone modifications. A recently established method called CUT&Tag uses a Protein-A Tn5 transposase fusion protein, which cuts the genome and inserts adapter sequences nearby the target protein. Throughout most of the CUT&Tag procedure, cells are held on concanavalin A (con A)-conjugated magnetic beads. Proper holding of cells would be decisive for the accessibility of Tn5 to the chromatin, and efficacy of the procedure of washing cells. However, BioMagPlus ConA magnetic beads, used in the original CUT&Tag protocol, often exhibit poor suspendability and severe aggregation. Here, we compared the BioMag beads and Dynabeads magnetic particles of which conjugation of con A was done by our hands, and examined the performance of these magnetic beads in CUT&Tag. Among tested, one of the Dynabeads, MyOne-T1, kept excessive suspendability in a buffer even after overnight incubation. Furthermore, the MyOne-T1 beads notably improved the sensitivity in CUT&Tag assay for H3K4me3. In conclusion, the arrangement and the selection of MyOne-T1 refine the suspendability of beads, which improves the association of chromatin with Tn5, which enhances the sensitivity in CUT&Tag assay.

Ultrastructural and chemical evidences of heterogeneity and hormone dependence of certain glycocalyces

1978 ◽  
Vol 36 (2) ◽  
pp. 322-323
Author(s):  
B. Monis ◽  
D. Lis ◽  
I. Parlanti ◽  
A. R. Eynard ◽  
M. A. Valentich ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Concanavalin A ◽  
Ruthenium Red ◽  
Transitional Epithelium ◽  
Membrane Material ◽  
Hormone Dependence ◽  
Cellulose Acetate Electrophoresis ◽  
Electrophoretic Data ◽  
Fat Globule ◽  
Collecting Tubules

We are gathering evidences which indicate ultrastructural variations and chemical heterogeneity of certain glycocalyces as well as hormone dependence of some of them. Thus, in the lumenal glycocalyx of renal collecting tubules of the guinea-pig granular and filamentous structures were seen (1, fig. 1). By isolation, chemical analysis and cellulose acetate electrophoresis in various buffers of tubular membrane material, glycopeptides and glycosaminoglycans were identified (fig. 2).Guinea-pig and rat transitional epithelium of urinary tract showed a filamentous lumenal glycocalyx demonstrable with ruthenium red (fig. 3) but which only in part stained with concanavalin A. Chemical and electrophoretic data indicated that urothelium contains glycoproteins, glycosaminoglycans and glycolipids.The glycocalyx of the fat globule membrane of milk of several species has a granular appearance as shown by cationic dyes and by concanavalin A (2, 3, fig. 4 and 5). Also, several glycoproteins were isolated and identified on polyacrilamide gel electrophoresis (fig. 6). Glycosaminoglycans and certain glycolipids such as sulfatides were chemically identified in this glycocalyx.

A magnetic super lattice

1987 ◽  
Vol 45 ◽  
pp. 360-361 ◽  
Author(s):  
M.D. Bentzon ◽  
J. v. Wonterghem ◽  
A. Thölén
Keyword(s):  
Thermal Decomposition ◽  
Oleic Acid ◽  
Magnetic Fluid ◽  
Magnetic Particles ◽  
Carbon Film ◽  
Thick Layer ◽  
Amorphous Iron ◽  
Super Lattice ◽  
Carrier Liquid ◽  
Median Diameter

We report on the oxidation of a magnetic fluid. The oxidation results in magnetic super lattice crystals. The “atoms” are hematite (α-Fe2O3) particles with a diameter ø = 6.9 nm and they are covered with a 1-2 nm thick layer of surfactant molecules.Magnetic fluids are homogeneous suspensions of small magnetic particles in a carrier liquid. To prevent agglomeration, the particles are coated with surfactant molecules. The magnetic fluid studied in this work was produced by thermal decomposition of Fe(CO)5 in Declin (carrier liquid) in the presence of oleic acid (surfactant). The magnetic particles consist of an amorphous iron-carbon alloy. For TEM investigation a droplet of the fluid was added to benzine and a carbon film on a copper net was immersed. When exposed to air the sample starts burning. The oxidation and electron irradiation transform the magnetic particles into hematite (α-Fe2O3) particles with a median diameter ø = 6.9 nm.

EFFECT OF APPLICATION OF FIELDS ON THE DOMAIN STRUCTURE IN SMALL REGULARLY SHAPED MAGNETIC PARTICLES

1988 ◽  
Vol 49 (C8) ◽  
pp. C8-1817-C8-1818 ◽  
Author(s):  
S. McVitie ◽  
J. N. Chapman ◽  
S. J. Hefferman ◽  
W. A. P. Nicholson
Keyword(s):  
Domain Structure ◽  
Magnetic Particles

Die Aktivierung IL6/gp130 abhängiger Signalwege in Hepatozyten schützt die Leber vor Concanavalin A induzierter Hepatitis

2015 ◽  
Vol 41 (08) ◽  
Author(s):  
C Klein ◽  
T Wuestefeld ◽  
M Miyazaki ◽  
MP Manns ◽  
C Trautwein
Keyword(s):  
Concanavalin A
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