Growth and immunogenicity of foot-and-mouth disease virus in baby hamster kidney cells adapted to and continuously grown in a serum-free chemically defined media

1975 ◽  
Vol 17 (5) ◽  
pp. 765-778 ◽  
Author(s):  
L. D. Tomei ◽  
C. J. Issel
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Serum Free ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Mouth Disease Virus ◽  
Baby Hamster Kidney Cells

scholarly journals A study of the heterogeneous 37s ribonucleic acid induced by foot-and-mouth-disease virus

1968 ◽  
Vol 107 (3) ◽  
pp. 395-401 ◽  
Author(s):  
T. F. Wild ◽  
S J Martin ◽  
F. Brown
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Virus Particles ◽  
Mouth Disease Virus ◽  
Heterogeneous Nature ◽  
Foot And Mouth ◽  
Sepharose 4B ◽  
Baby Hamster Kidney Cells

1. The 37s RNA induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus was examined on sucrose gradients and by filtration through Sepharose 4B. 2. The RNA sedimented faster (37s) and as a broader band than the 35s RNA from purified virus. 3. Treatment with deoxyribonuclease, Pronase or amylase did not alter the sedimentation profile of the 37s RNA. 4. Treatment of individual fractions of the RNA with phenol, dimethyl sulphoxide or methylCellosolve did not decrease the sedimentation rate of the faster-sedimenting molecules. 5. Sedimentation in sucrose gradients of different ionic strengths or containing EDTA had no effect on the heterogeneous nature of the profile. 6. On filtration through Sepharose 4B columns, the 37s virus-induced RNA was eluted before viral RNA. 7. Only 20% of the rapidly sedimenting RNA was incorporated into complete virus particles.

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