scholarly journals A study of the mechanism of cyanogen bromide activation of cellulose

1972 ◽  
Vol 14 (6) ◽  
pp. 1039-1044 ◽  
Author(s):  
G. J. Bartling ◽  
H. D. Brown ◽  
L. J. Forrester ◽  
M. T. Koes ◽  
A. N. Mather ◽  
...  
Keyword(s):  
Cyanogen Bromide ◽  
Cyanogen Bromide Activation

scholarly journals An Alternative Coupling Procedure for Preparing Activated Sepharose for Affinity Chromatography of Penicillinase

1976 ◽  
Vol 29 (4) ◽  
pp. 305 ◽  
Author(s):  
RG Coombe ◽  
AM George
Keyword(s):  
Affinity Chromatography ◽  
Room Temperature ◽  
Cyanogen Bromide ◽  
Dry Weight ◽  
Enzymic Activity ◽  
Covalent Attachment ◽  
Affinity Column ◽  
Coupling Procedure ◽  
Coupling Techniques ◽  
Cyanogen Bromide Activation

Most applications of affinity chromatography employ the cyanogen bromide activation scheme first devised by Axtm et al. (1967). Porath and Sundberg (1972) reported an alternative procedure in which phloroglucinol and divinylsulphone are used in activating reactions. The advantages of this scheme and parameters relevant to the activating reactions are reported here. Conditions for the attachment of various ligand molecules to sepharose using a divinylsulphone activation method are defined, and a comparison with cyanogen bromide activating and coupling techniques is drawn. a-Chymotrypsin is immobilized by covalent attachment to activated sepharose. The optimum coupling pH is 8� 0-8� 6 and the reaction is virtually complete after 20 h at room temperature. Conjugates containing as much as 2 g of enzyme per gram dry weight of polymer were obtained. The immobilized enzyme retained 41 % of the free enzymic activity. An affinity column of divinylsulphone-activated methicillin-sepharose was used to demonstrate the reversible adsorption of penicillinase.

Improved method for the cyanogen bromide activation of agarose beads

1979 ◽  
Vol 172 (1) ◽  
pp. 221-226 ◽  
Author(s):  
Gunther Kümel ◽  
Heiner Daus ◽  
Harald Mauch
Keyword(s):  
Cyanogen Bromide ◽  
Improved Method ◽  
Agarose Beads ◽  
Cyanogen Bromide Activation

Cyanogen bromide activation of polysaccharides

1977 ◽  
Vol 79 (1-2) ◽  
pp. 513-525 ◽  
Author(s):  
Ronald L. Schnaar ◽  
T.Flint Sparks ◽  
Saul Roseman
Keyword(s):  
Cyanogen Bromide ◽  
Cyanogen Bromide Activation

Investigations on the cyanogen bromide activation of xanthan gum

1985 ◽  
Vol 17 (4) ◽  
pp. 334-338 ◽  
Author(s):  
John F. Kennedy ◽  
Meriam Adam Hussain
Keyword(s):  
Xanthan Gum ◽  
Cyanogen Bromide ◽  
Cyanogen Bromide Activation

Cyanogen Bromide Activation and Deactivation of Agarose

1987 ◽  
Vol 501 (1 Enzyme Engine) ◽  
pp. 420-425 ◽  
Author(s):  
JULIAN SERRANO ◽  
ANTONIO BALLESTEROS
Keyword(s):  
Cyanogen Bromide ◽  
Cyanogen Bromide Activation

SECOND ANTIBODY CHEMICALLY LINKED TO CELLULOSE FOR THE SEPARATION OF BOUND AND FREE HORMONE: AN IMPROVEMENT OVER SOLUBLE SECOND ANTIBODY IN GONADOTROPHIN RADIOIMMUNOASSAY

1976 ◽  
Vol 81 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Ph. Koninckx ◽  
R. Bouillon ◽  
P. De Moor
Keyword(s):  
Detection Limit ◽  
Protein Concentration ◽  
Cyanogen Bromide ◽  
Solid Phase ◽  
Phase System ◽  
Cellulose Matrix ◽  
Classical Technique ◽  
Definite Improvement ◽  
Cyanogen Bromide Activation

ABSTRACT Coupling the second antibody to a solid pase (DASP) was found to be a definite improvement over the classical technique of soluble second antibody (DA) separation of bound and free hormone in the radioimmunoassays of gonadotrophins in plasma or in serum. The duration (2.5 to 7.5 min) and the pH (10.5 to 12.5) of the cyanogen bromide activation of microcristalline cellulose did not affect the coupling capacity or the immunoreactivity of the second antibody, nor did it augment aspecific adsorption of free gonadotrophin on the cellulose matrix. Precision of duplicates was comparable for both methods but the accuracy and the detection limit of the solid phase system were higher due to a better separation of bound and free hormone, to the lower blank value and to the absence of aspecific interference of protein concentration. Cost was lower since much less second antibody was needed in the solid phase system than in the soluble system and since the experimental procedures were simplified by the absence of prozoning effect and the solid nature of the cellulose matrix.

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