scholarly journals Foam concentration of streptokinase from crude culture filtrates

1968 ◽  
Vol 10 (4) ◽  
pp. 551-552 ◽  
Author(s):  
Björn Holmström
Keyword(s):  
Culture Filtrates ◽  
Foam Concentration

scholarly journals Bacterial Culture Filtrates Antinematode Activity of Abomasum Bacterial Culture Filtrates against Haemonchus contortus in Small Ruminants

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1843
Author(s):  
Asfa Nazish ◽  
Fozia ◽  
Baharullah Khattak ◽  
Taj Ali Khan ◽  
Ijaz Ahmad ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Bacterial Culture ◽  
Small Ruminants ◽  
Adult Mortality ◽  
Comamonas Testosteroni ◽  
Bacterial Isolates ◽  
Egg Hatch ◽  
Nematode Parasites ◽  
Culture Filtrates ◽  
Nematode Mortality

Haemonchosis is a parasitic disease of small ruminants that adversely affects livestock production. Haemonchus contortus is one of the most prevalent nematode parasites that infect the abomasum of small ruminants. This parasite reduces milk production, overall growth and sometimes causes the death of the infected animals. The evaluation of the biocontrol potential of some abomasum bacterial isolates against H. contortus is investigated in this study. Out of which, three isolates—Comamonas testosteroni, Comamonas jiangduensis, Pseudomonas weihenstephanesis—show significant effect against the nematode L3, adult, and egg hatch inhibition assays. Various concentrations of metabolites from these bacteria are prepared and applied in different treatments compared with control. In the case of adult mortality assay, 50% metabolites of C. testosteroni and P. weihenstephanesis show 46% adult mortality, whereas C. jiangduensis shows 40% mortality. It is observed that decreasing the concentration of bacterial metabolite, lowers nematode mortality. The minimum nematode mortality rate is recorded at the lowest filtrates concentration of all the bacterial isolates. The same trend is observed in egg hatch inhibition assay, where the higher concentration of bacterial culture filtrates shows 100% inhibition of H. contortus egg. It is concluded that the effect of bacterial culture filtrates against H. contortus is dose-dependent for their activity against nematode L3, adult, and inhibition of egg hatchment.

scholarly journals Characterization of a Cytotoxic Factor in Culture Filtrates of Serratia marcescens

2002 ◽  
Vol 70 (3) ◽  
pp. 1121-1128 ◽  
Author(s):  
Kent B. Marty ◽  
Christopher L. Williams ◽  
Linda J. Guynn ◽  
Michael J. Benedik ◽  
Steven R. Blanke
Keyword(s):  
Cytotoxic Activity ◽  
Serratia Marcescens ◽  
Mammalian Cells ◽  
Divalent Cations ◽  
Recombinant Expression ◽  
Cytotoxic Factor ◽  
Culture Filtrates ◽  
Type Strains

ABSTRACT Serratia marcescens culture filtrates have been reported to be cytotoxic to mammalian cells. Using biochemical and genetic approaches, we have identified a major source of this cytotoxic activity. Both heat and protease treatments abrogated the cytotoxicity of S. marcescens culture filtrates towards HeLa cells, suggesting the involvement of one or more protein factors. A screen for in vitro cytotoxic activity revealed that S. marcescens mutant strains that are deficient in production of a 56-kDa metalloprotease are significantly less cytotoxic to mammalian cells. Cytotoxicity was significantly reduced when culture filtrates prepared from wild-type strains were pretreated with either EDTA or 1,10-phenanthroline, which are potent inhibitors of the 56-kDa metalloprotease. Furthermore, cytotoxic activity was restored when the same culture filtrates were incubated with zinc divalent cations, which are essential for enzymatic activity of the 56-kDa metalloprotease. Finally, recombinant expression of the S. marcescens 56-kDa metalloprotease conferred a cytotoxic phenotype on the culture filtrates of a nonpathogenic Escherichia coli strain. Collectively, these data suggest that the 56-kDa metalloprotease contributes significantly to the in vitro cytotoxic activity commonly observed in S. marcescens culture filtrates.

scholarly journals Effect of Lactobacillus paracasei Culture Filtrates and Artichoke Polyphenols on Cytokine Production by Dendritic Cells

Nutrients ◽  
2016 ◽  
Vol 8 (10) ◽  
pp. 635 ◽  
Author(s):  
Angelo Sisto ◽  
Diomira Luongo ◽  
Lucia Treppiccione ◽  
Palmira De Bellis ◽  
Donato Di Venere ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cytokine Production ◽  
Lactobacillus Paracasei ◽  
Culture Filtrates

scholarly journals Tanzawaic acids I–L: Four new polyketides from Penicillium sp. IBWF104-06

2014 ◽  
Vol 10 ◽  
pp. 251-258 ◽  
Author(s):  
Louis P Sandjo ◽  
Eckhard Thines ◽  
Till Opatz ◽  
Anja Schüffler
Keyword(s):  
Soil Sample ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Fungal Strain ◽  
Conidial Germination ◽  
Penicillium Sp ◽  
Culture Filtrates ◽  
Blast Fungus ◽  
New Compounds

Four new polyketides have been identified in culture filtrates of the fungal strain Penicillium sp. IBWF104-06 isolated from a soil sample. They are structurally based on the same trans-decalinpentanoic acid skeleton as tanzawaic acids A–H. One of the new compounds was found to inhibit the conidial germination in the rice blast fungus Magnaporthe oryzae at concentrations of 25 μg/mL.

scholarly journals The Liquid Culture Filtrates of Entomogenous fungus Paecilomyces tenuipes and Its Glycoprotein Constituent Protects against Anemia in Mice Treated with 5-Fluorouracil

2008 ◽  
Vol 31 (8) ◽  
pp. 1565-1573 ◽  
Author(s):  
Takanobu Takata ◽  
Tomoaki Tanaka ◽  
Nobuo Yahagi ◽  
Remiko Yahagi ◽  
Hideyuki Tsuchida ◽  
...  
Keyword(s):  
Liquid Culture ◽  
Entomogenous Fungus ◽  
Culture Filtrates
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