scholarly journals Sphingolipids contribute to acetic acid resistance inZygosaccharomyces bailii

2015 ◽  
Vol 113 (4) ◽  
pp. 744-753 ◽  
Author(s):  
Lina Lindahl ◽  
Samuel Genheden ◽  
Leif A. Eriksson ◽  
Lisbeth Olsson ◽  
Maurizio Bettiga
Keyword(s):  
Acetic Acid ◽  
Acid Resistance ◽  
Acetic Acid Resistance

scholarly journals The dual role of candida glabrata drug:H+ antiporter CgAqr1 (ORF CAGL0J09944g) in antifungal drug and acetic acid resistance

2013 ◽  
Vol 4 ◽  
Author(s):  
Catarina Costa ◽  
André Henriques ◽  
Carla Pires ◽  
Joana Nunes ◽  
Michiyo Ohno ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Candida Glabrata ◽  
Acid Resistance ◽  
Dual Role ◽  
Antifungal Drug ◽  
Acetic Acid Resistance

scholarly journals Putative ABC Transporter Responsible for Acetic Acid Resistance in Acetobacter aceti

2006 ◽  
Vol 72 (1) ◽  
pp. 497-505 ◽  
Author(s):  
Shigeru Nakano ◽  
Masahiro Fukaya ◽  
Sueharu Horinouchi
Keyword(s):  
Acetic Acid ◽  
Abc Transporter ◽  
Acid Resistance ◽  
Acetic Acid Bacteria ◽  
Electrophoretic Analysis ◽  
Nucleotide Sequencing ◽  
Multicopy Plasmid ◽  
Coding Region ◽  
Acetobacter Aceti ◽  
Acetic Acid Resistance

ABSTRACT Two-dimensional gel electrophoretic analysis of the membrane fraction of Acetobacter aceti revealed the presence of several proteins that were produced in response to acetic acid. A 60-kDa protein, named AatA, which was mostly induced by acetic acid, was prepared; aatA was cloned on the basis of its NH2-terminal amino acid sequence. AatA, consisting of 591 amino acids and containing ATP-binding cassette (ABC) sequences and ABC signature sequences, belonged to the ABC transporter superfamily. The aatA mutation with an insertion of the neomycin resistance gene within the aatA coding region showed reduced resistance to acetic acid, formic acid, propionic acid, and lactic acid, whereas the aatA mutation exerted no effects on resistance to various drugs, growth at low pH (adjusted with HCl), assimilation of acetic acid, or resistance to citric acid. Introduction of plasmid pABC101 containing aatA under the control of the Escherichia coli lac promoter into the aatA mutant restored the defect in acetic acid resistance. In addition, pABC101 conferred acetic acid resistance on E. coli. These findings showed that AatA was a putative ABC transporter conferring acetic acid resistance on the host cell. Southern blot analysis and subsequent nucleotide sequencing predicted the presence of aatA orthologues in a variety of acetic acid bacteria belonging to the genera Acetobacter and Gluconacetobacter. The fermentation with A. aceti containing aatA on a multicopy plasmid resulted in an increase in the final yield of acetic acid.

Enhanced expression of aconitase raises acetic acid resistance inAcetobacter aceti

2004 ◽  
Vol 235 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Shigeru Nakano ◽  
Masahiro Fukaya ◽  
Sueharu Horinouchi
Keyword(s):  
Acetic Acid ◽  
Acid Resistance ◽  
Acetic Acid Resistance ◽  
Enhanced Expression

Acetic Acid Fermentation ofAcetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation

2010 ◽  
Vol 74 (8) ◽  
pp. 1591-1597 ◽  
Author(s):  
Watchara KANCHANARACH ◽  
Gunjana THEERAGOOL ◽  
Taketo INOUE ◽  
Toshiharu YAKUSHI ◽  
Osao ADACHI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Acid Resistance ◽  
Acid Fermentation ◽  
Acetic Acid Fermentation ◽  
Acetic Acid Resistance

scholarly journals Improved Acetic Acid Resistance in Saccharomyces cerevisiae by Overexpression of theWHI2Gene Identified through Inverse Metabolic Engineering

2016 ◽  
Vol 82 (7) ◽  
pp. 2156-2166 ◽  
Author(s):  
Yingying Chen ◽  
Lisa Stabryla ◽  
Na Wei
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Metabolic Engineering ◽  
Acid Stress ◽  
Acid Resistance ◽  
Biofuel Production ◽  
Gene Target ◽  
Content Type ◽  
Inverse Metabolic Engineering ◽  
Acetic Acid Resistance

ABSTRACTDevelopment of acetic acid-resistantSaccharomyces cerevisiaeis important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge due to limited information on effective genetic perturbation targets for improving acetic acid resistance in the yeast. This study employed a genomic-library-based inverse metabolic engineering approach to successfully identify a novel gene target,WHI2(encoding a cytoplasmatic globular scaffold protein), which elicited improved acetic acid resistance inS. cerevisiae. Overexpression ofWHI2significantly improved glucose and/or xylose fermentation under acetic acid stress in engineered yeast. TheWHI2-overexpressing strain had 5-times-higher specific ethanol productivity than the control in glucose fermentation with acetic acid. Analysis of the expression ofWHI2gene products (including protein and transcript) determined that acetic acid induced endogenous expression of Whi2 inS. cerevisiae. Meanwhile, thewhi2Δ mutant strain had substantially higher susceptibility to acetic acid than the wild type, suggesting the important role of Whi2 in the acetic acid response inS. cerevisiae. Additionally, overexpression ofWHI2and of a cognate phosphatase gene,PSR1, had a synergistic effect in improving acetic acid resistance, suggesting that Whi2 might function in combination with Psr1 to elicit the acetic acid resistance mechanism. These results improve our understanding of the yeast response to acetic acid stress and provide a new strategy to breed acetic acid-resistant yeast strains for renewable biofuel production.

Escherichia coli O157:H7 Strains Isolated from Environmental Sources Differ Significantly in Acetic Acid Resistance Compared with Human Outbreak Strains†‡

2009 ◽  
Vol 72 (3) ◽  
pp. 503-509 ◽  
Author(s):  
DEOG-HWAN OH ◽  
YOUWEN PAN ◽  
ELAINE BERRY ◽  
MICHAEL COOLEY ◽  
ROBERT MANDRELL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Acetic Acid Solution ◽  
Acid Resistance ◽  
Escherichia Coli O157 ◽  
Limited Information ◽  
E Coli ◽  
Log Reduction ◽  
Acetic Acid Resistance ◽  
Acidified Foods

A number of studies on the influence of acid on Escherichia coli O157:H7 have shown considerable strain differences, but limited information has been reported to compare the acid resistance based on the different sources of E. coli O157:H7 isolates. The purpose of this study was to determine the survival of E. coli O157:H7 strains isolated from five sources (foods, bovine carcasses, bovine feces, water, and human) in 400 mM acetic acid solutions under conditions that are typical of acidified foods. The isolates from bovine carcasses, feces, and water survived acetic acid treatment at pH 3.3 and 30°C significantly (P ≤ 0.05) better than did any food or human isolates. However, resistance to acetic acid significantly increased as temperature decreased to 15°C for a given pH, with little (P ≥ 0.05) difference among the different isolation sources. All groups of E. coli O157:H7 strains showed more than 1.8- to 4.5-log reduction at pH 3.3 and 30°C after 25 min. Significantly reduced (less than 1-log reduction) lethality for all E. coli O157:H7 strain mixtures was observed when pH increased to 3.7 or 4.3, with little difference in acetic acid resistance among the groups. The addition of glutamate to the acetic acid solution or anaerobic incubation provided the best protection compared with the above conditions for all groups of isolates. These results suggest that temperature, pH, and atmospheric conditions are key factors in establishing strategies for improving the safety of acidified foods.

Towards Understanding the Acetic Acid Resistance inGluconacetobacter Europaeus

2008 ◽  
pp. 674-678 ◽  
Author(s):  
J. Trcek ◽  
H. Toyama ◽  
J. Czuba ◽  
A. Misiewicz ◽  
K. Matusushita
Keyword(s):  
Acetic Acid ◽  
Acid Resistance ◽  
Acetic Acid Resistance

Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria

2006 ◽  
Vol 70 (3) ◽  
pp. 366-373 ◽  
Author(s):  
Janja Trcek ◽  
Hirohide Toyama ◽  
Jerzy Czuba ◽  
Anna Misiewicz ◽  
Kazunobu Matsushita
Keyword(s):  
Acetic Acid ◽  
Acid Resistance ◽  
Acetic Acid Bacteria ◽  
Acetic Acid Resistance
Sign in / Sign up

Export Citation Format

Share Document