Effect of anion structures on cholinium ionic liquids pretreatment of rice straw and the subsequent enzymatic hydrolysis

2014 ◽  
Vol 112 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Xue-Dan Hou ◽  
Jie Xu ◽  
Ning Li ◽  
Min-Hua Zong
Keyword(s):  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Ionic Liquids Pretreatment

scholarly journals Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Deep Eutectic Solvent ◽  
Solvent System ◽  
Cyclic Ether ◽  
Cellulolytic Enzymes ◽  
Ammonium Bromide ◽  
Selective Delignification ◽  
Cellulose Iβ ◽  
Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

Enzymatic Hydrolysis of Ammonia-Treated Rice Straw

2003 ◽  
Vol 105 (1-3) ◽  
pp. 155-164 ◽  
Author(s):  
Betzabé Sulbarán-De-Ferrer ◽  
Marielena Aristiguieta ◽  
Bruce E. Dale ◽  
Alexis Ferrer ◽  
Graciela Ojeda-De-Rodriguez
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Hydrolysis Of

scholarly journals Enzymatic hydrolysis of chicken viscera proteins in the presence of an ionic liquid as a strategy to improve the antioxidant properties of the hydrolysates

2019 ◽  
Author(s):  
Vitor Geniselli da Silva ◽  
Ruann Janser Soares de Castro
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Protein Hydrolysates ◽  
Tetramethylammonium Bromide ◽  
Chicken Viscera ◽  
Hydrolysis Of

Aiming to explore the use of ionic liquids (ILs) not yet described in the literature, this work evaluated the hydrolysis of proteins from chicken viscera using the protease Alcalase modified and unmodified by the IL tetramethylammonium bromide. The protein hydrolysates produced in the presence of the IL presented values of antioxidant activities 40% higher than the hydrolysates obtained without IL. In addition, with the presence of the IL, it was possible to obtain protein hydrolysates from chicken viscera with similar antioxidant activities, compared to the protein hydrolysates produced without IL, using 1/3 of the amount of enzyme.

scholarly journals A novel high performance metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

2020 ◽  
Author(s):  
Shohreh Ariaeenejad ◽  
Atefeh Sheykhabdolahzadeh ◽  
Morteza Maleki ◽  
Kaveh Kavousi ◽  
Mehdi Foroozandeh Shahraki ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Specific Activity ◽  
Agricultural Waste ◽  
Extreme Temperature ◽  
In Silico Analysis ◽  
Cellulose Content ◽  
Biomass Hydrolysis ◽  
Harsh Condition

Abstract Background: Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a wide search for novel effective enzymes to improve the exploitation of lignocellulose, among which the importance of thermostable and halotolerant cellulase enzymes with high pH performance is significant. Results: The primary aim of this study was to discover a novel alkali-thermostable endo-β-1,4-glucanase from the sheep rumen metagenome. Using a multi-step in-silico analysis, primary candidates with desired properties were found and subjected to cloning, expression, and purification followed by functional and structural characterization. The enzymes' kinetic parameters, including V max , Km, and specific activity, were calculated. The PersiCel4 demonstrated its optimum activity at pH 8.5 and a temperature of 85°C and was able to retain more than 70% of its activity after 150 hours of storage at 85°C. Furthermore, this enzyme was able to maintain its catalytic activity in the presence of different concentrations of NaCl, MgCl 2 , CaCl 2 , and MnCl 2 . Our results showed that treatment with MnCl 2 could enhance the enzyme’s activity by 89%. PersiCel4 was ultimately used for enzymatic hydrolysis of autoclave pretreated rice straw, the most abundant agricultural waste with rich cellulose content. In autoclave treated rice straw, enzymatic hydrolysis with the PersiCel4 increased the release of reducing sugar up to 260% after 72 hours in the harsh condition ( T= 85°C, pH = 8.5). Conclusion: Considering the urgent demand for stable cellulases that are operational on extreme temperature and pH conditions and due to several proposed distinctive characteristics of PersiCel4, it can be used in the harsh condition for bioconversion of lignocellulosic biomass.

Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica

2012 ◽  
Vol 123 ◽  
pp. 636-645 ◽  
Author(s):  
Kyoung-Mi Lee ◽  
Dayanand Kalyani ◽  
Manish Kumar Tiwari ◽  
Tae-Su Kim ◽  
Saurabh Sudha Dhiman ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Yarrowia Lipolytica ◽  
Hydrolysis Of ◽  
Yeast Yarrowia Lipolytica

Enzymatic hydrolysis of penicillin G in ionic liquids

2008 ◽  
Vol 136 ◽  
pp. S391
Author(s):  
Mai Ngoc Lan ◽  
Sung Ho Ha ◽  
Yoon-Mo Koo
Keyword(s):  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Penicillin G ◽  
Hydrolysis Of

Effect of iron salt type and dosing mode on Fenton-based pretreatment of rice straw for enzymatic hydrolysis

2018 ◽  
Vol 265 ◽  
pp. 394-398 ◽  
Author(s):  
Yu-Yan Gan ◽  
Si-Li Zhou ◽  
Xiao Dai ◽  
Han Wu ◽  
Zi-Yao Xiong ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Iron Salt
Sign in / Sign up

Export Citation Format

Share Document