Demonstration of in situ product recovery of butyric acid via CO2-facilitated pH swings and medium development in two-phase partitioning bioreactors

2013 ◽  
Vol 111 (3) ◽  
pp. 537-544 ◽  
Author(s):  
Eric C. Peterson ◽  
Andrew J. Daugulis
Keyword(s):  
Butyric Acid ◽  
Product Recovery ◽  
Two Phase ◽  
Phase Partitioning ◽  
Medium Development ◽  
In Situ Product Recovery

scholarly journals Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3356 ◽  
Author(s):  
Francisco Aguilar ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Liquid Phase ◽  
Product Recovery ◽  
Expanded Bed Adsorption ◽  
Biotechnological Production ◽  
Promising Alternative ◽  
Phase Partitioning ◽  
In Situ Product Recovery ◽  
Solid Liquid ◽  
In Situ Recovery

The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L−1 and productivity of 3.2 mg L−1 h−1. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules.

scholarly journals A comparison of the energy use of in situ product recovery techniques for the Acetone Butanol Ethanol fermentation

2016 ◽  
Vol 220 ◽  
pp. 590-600 ◽  
Author(s):  
Victoria Outram ◽  
Carl-Axel Lalander ◽  
Jonathan G.M. Lee ◽  
E. Timothy Davis ◽  
Adam P. Harvey
Keyword(s):  
Ethanol Fermentation ◽  
Energy Use ◽  
Product Recovery ◽  
In Situ Product Recovery ◽  
Recovery Techniques ◽  
Acetone Butanol Ethanol

Improved enzymatic synthesis of N-carbamoyl-D-phenylalanine with in situ product recovery

2011 ◽  
Vol 16 (3) ◽  
pp. 611-616 ◽  
Author(s):  
Z. Zhou ◽  
Z. Yao ◽  
H. Q. Wang ◽  
H. Xu ◽  
P. Wei ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Product Recovery ◽  
In Situ Product Recovery

Development of reactive extraction systems for itaconic acid: a step towards in situ product recovery for itaconic acid fermentation

RSC Advances ◽  
2014 ◽  
Vol 4 (85) ◽  
pp. 45029-45039 ◽  
Author(s):  
Guneet Kaur ◽  
Kathy Elst
Keyword(s):  
Process Optimization ◽  
Itaconic Acid ◽  
Acid Production ◽  
Product Recovery ◽  
Reactive Extraction ◽  
Acid Fermentation ◽  
In Situ Product Recovery ◽  
Product Separation ◽  
Important Means

Process optimization by integration of bioconversion with product separation and recovery i.e. in situ product recovery (ISPR) is an important means to develop a sustainable and petrochemical-competitive biotechnological method for itaconic acid production.

In situ product recovery of single-chain antibodies in a membrane bioreactor

2014 ◽  
Vol 111 (8) ◽  
pp. 1566-1576 ◽  
Author(s):  
Kristina Meier ◽  
Frederike Carstensen ◽  
Christoph Scheeren ◽  
Lars Regestein ◽  
Matthias Wessling ◽  
...  
Keyword(s):  
Membrane Bioreactor ◽  
Product Recovery ◽  
Single Chain ◽  
In Situ Product Recovery ◽  
Single Chain Antibodies

In‐situ product recovery as a strategy for bioprocess integration and depletion of inhibitory products

2021 ◽  
Author(s):  
Ulises A. Salas‐Villalobos ◽  
Rígel V. Gómez‐Acata ◽  
Josefina Castillo‐Reyna ◽  
Oscar Aguilar
Keyword(s):  
Product Recovery ◽  
In Situ Product Recovery
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