Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition

2013 ◽  
Vol 110 (12) ◽  
pp. 3244-3257 ◽  
Author(s):  
Nuno Carinhas ◽  
Tiago M. Duarte ◽  
Laura C. Barreiro ◽  
Manuel J. T. Carrondo ◽  
Paula M. Alves ◽  
...  
Keyword(s):  
Phase Transition ◽  
Cho Cell ◽  
Cell Clones ◽  
Butyrate Treatment ◽  
Culture Phase ◽  
Metabolic Signatures

Productivity and Quality of Recombinant Proteins Produced by Stable CHO Cell Clones can be Predicted by Transient Expression in HEK Cells

2012 ◽  
Vol 54 (2) ◽  
pp. 497-503 ◽  
Author(s):  
Carolin Diepenbruck ◽  
Matthias Klinger ◽  
Thomas Urbig ◽  
Patrick Baeuerle ◽  
Rüdiger Neef
Keyword(s):  
Transient Expression ◽  
Recombinant Proteins ◽  
Cho Cell ◽  
Hek Cells ◽  
Cell Clones

Screening of CHO cell clones expressing histidine-tagged major S hepatitis B surface protein using a semi-quantitative PCR protocol

2003 ◽  
Vol 114 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Carlos Otávio Alves Vianna ◽  
Sergio da Silva e Mouta Junior ◽  
Gerson de Oliveira da Silva ◽  
Marcos da Silva Freire ◽  
Marcia Terezinha Baroni de Moraes
Keyword(s):  
Hepatitis B ◽  
Quantitative Pcr ◽  
Surface Protein ◽  
Cho Cell ◽  
Cell Clones ◽  
Semi Quantitative Pcr

scholarly journals Application of a genome-scale model in tandem with enzyme assays for identification of metabolic signatures of high and low CHO cell producers

2019 ◽  
Vol 9 ◽  
pp. e00097 ◽  
Author(s):  
Cyrielle Calmels ◽  
Solène Arnoult ◽  
Bassem Ben Yahia ◽  
Laetitia Malphettes ◽  
Mikael Rørdam Andersen
Keyword(s):  
Scale Model ◽  
Enzyme Assays ◽  
Cho Cell ◽  
A Genome ◽  
Genome Scale ◽  
Genome Scale Model ◽  
Metabolic Signatures

An Expression Vector Encoding alacZReporter Gene Facilitates Identification of Stable, High-Producing CHO Cell Clones

Plasmid ◽  
1997 ◽  
Vol 37 (2) ◽  
pp. 155-158
Author(s):  
Trudy H. Grossman ◽  
Jorge E. Godoy ◽  
Ernest S. Kawasaki ◽  
Marcia S. Osburne
Keyword(s):  
Expression Vector ◽  
Cho Cell ◽  
Cell Clones

Glycan‐masking hemagglutinin antigens from stable CHO cell clones for H5N1 avian influenza vaccine development

2018 ◽  
Vol 116 (3) ◽  
pp. 598-609 ◽  
Author(s):  
Ting‐Hsuan Chen ◽  
Wen‐Chun Liu ◽  
Chia‐Ying Lin ◽  
Chia‐Chyi Liu ◽  
Jia‐Tsrong Jan ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza Vaccine ◽  
Vaccine Development ◽  
Cho Cell ◽  
H5n1 Avian Influenza ◽  
Cell Clones

Microstructure of laser-irradiated, conducting Kapton polyimide

1995 ◽  
Vol 53 ◽  
pp. 502-503
Author(s):  
D. L. Callahan ◽  
Z. Ball ◽  
H. M. Phillips ◽  
R. Sauerbrey
Keyword(s):  
Electron Microscopy ◽  
Phase Transition ◽  
Transmission Electron Microscopy ◽  
Cross Section ◽  
Film Surface ◽  
Cross Sectional ◽  
General Utility ◽  
Transmission Electron ◽  
Considerable Debate ◽  
Potential Applications

Ultraviolet laser-irradiation can be used to induce an insulator-to-conductor phase transition on the surface of Kapton polyimide. Such structures have potential applications as resistors or conductors for VLSI applications as well as general utility electrodes. Although the percolative nature of the phase transformation has been well-established, there has been little definitive work on the mechanism or extent of transformation. In particular, there has been considerable debate about whether or not the transition is primarily photothermal in nature, as we propose, or photochemical. In this study, cross-sectional optical microscopy and transmission electron microscopy are utilized to characterize the nature of microstructural changes associated with the laser-induced pyrolysis of polyimide.Laser-modified polyimide samples initially 12 μm thick were prepared in cross-section by standard ultramicrotomy. Resulting contraction in parallel to the film surface has led to distortions in apparent magnification. The scale bars shown are calibrated for the direction normal to the film surface only.

The structure of membranes and membrane proteins embedded in amorphous ice

1992 ◽  
Vol 50 (1) ◽  
pp. 432-433
Author(s):  
Uwe Lücken ◽  
Joachim Jäger
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Membrane Proteins ◽  
Phase Transition Temperature ◽  
Lipid Vesicles ◽  
Freezing Stress ◽  
Amorphous Ice ◽  
Different Temperatures ◽  
Band Pass ◽  
Lipid Polymorphism

TEM imaging of frozen-hydrated lipid vesicles has been done by several groups Thermotrophic and lyotrophic polymorphism has been reported. By using image processing, computer simulation and tilt experiments, we tried to learn about the influence of freezing-stress and defocus artifacts on the lipid polymorphism and fine structure of the bilayer profile. We show integrated membrane proteins do modulate the bilayer structure and the morphology of the vesicles.Phase transitions of DMPC vesicles were visualized after freezing under equilibrium conditions at different temperatures in a controlled-environment vitrification system. Below the main phase transition temperature of 24°C (Fig. 1), vesicles show a facetted appearance due to the quasicrystalline areas. A gradual increase in temperature leads to melting processes with different morphology in the bilayer profile. Far above the phase transition temperature the bilayer profile is still present. In the band-pass-filtered images (Fig. 2) no significant change in the width of the bilayer profile is visible.

Sign in / Sign up

Export Citation Format

Share Document