Directed evolution of a highly efficient cellobiose utilizing pathway in an industrialSaccharomyces cerevisiaestrain

2013 ◽  
Vol 110 (11) ◽  
pp. 2874-2881 ◽  
Author(s):  
Yongbo Yuan ◽  
Huimin Zhao
Keyword(s):  
Directed Evolution ◽  
Highly Efficient

Directed Evolution of the Enzyme Monoamine Oxidase (MAO-N): Highly Efficient Chemo-enzymatic Deracemisation of the Alkaloid (±)-Crispine A

ChemCatChem ◽  
2012 ◽  
Vol 4 (9) ◽  
pp. 1259-1261 ◽  
Author(s):  
Ian Rowles ◽  
Kirk J. Malone ◽  
Laura L. Etchells ◽  
Simon C. Willies ◽  
Nicholas J. Turner
Keyword(s):  
Monoamine Oxidase ◽  
Directed Evolution ◽  
Highly Efficient ◽  
Enzyme Monoamine Oxidase

scholarly journals Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers

2017 ◽  
Vol 30 (4) ◽  
pp. 333-345 ◽  
Author(s):  
Moshe Goldsmith ◽  
Nidhi Aggarwal ◽  
Yacov Ashani ◽  
Halim Jubran ◽  
Per Jr. Greisen ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Nerve Agent ◽  
Highly Efficient

scholarly journals An in vivo selection system with tightly regulated gene expression enables directed evolution of highly efficient enzymes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Parinthon Nearmnala ◽  
Manutsawee Thanaburakorn ◽  
Watanalai Panbangred ◽  
Pimchai Chaiyen ◽  
Narupat Hongdilokkul
Keyword(s):  
Directed Evolution ◽  
Selection Pressure ◽  
Catalytic Efficiency ◽  
Selection System ◽  
In Vivo Selection ◽  
Highly Efficient ◽  
Highly Active ◽  
Wide Range ◽  
Regulated Gene Expression

AbstractIn vivo selection systems are powerful tools for directed evolution of enzymes. The selection pressure of the systems can be tuned by regulating the expression levels of the catalysts. In this work, we engineered a selection system for laboratory evolution of highly active enzymes by incorporating a translationally suppressing cis repressor as well as an inducible promoter to impart stringent and tunable selection pressure. We demonstrated the utility of our selection system by performing directed evolution experiments using TEM β-lactamase as the model enzyme. Five evolutionary rounds afforded a highly active variant exhibiting 440-fold improvement in catalytic efficiency. We also showed that, without the cis repressor, the selection system cannot provide sufficient selection pressure required for evolving highly efficient TEM β-lactamase. The selection system should be applicable for the exploration of catalytic perfection of a wide range of enzymes.

scholarly journals Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas

2014 ◽  
Vol 111 (46) ◽  
pp. 16436-16441 ◽  
Author(s):  
Oscar Alvizo ◽  
Luan J. Nguyen ◽  
Christopher K. Savile ◽  
Jamie A. Bresson ◽  
Satish L. Lakhapatri ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Directed Evolution ◽  
Flue Gas ◽  
Carbon Capture ◽  
Highly Efficient

scholarly journals Highly Efficient and More General cis- and trans-Splicing Inteins through Sequential Directed Evolution

2011 ◽  
Vol 286 (39) ◽  
pp. 34440-34447 ◽  
Author(s):  
Julia H. Appleby-Tagoe ◽  
Ilka V. Thiel ◽  
Yi Wang ◽  
Yanfei Wang ◽  
Henning D. Mootz ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Highly Efficient ◽  
Trans Splicing ◽  
Cis And Trans

Highly efficient and selective photocatalytic CO2 to CO conversion in aqueous solution

2020 ◽  
Vol 56 (27) ◽  
pp. 3851-3854 ◽  
Author(s):  
Xiaomin Chai ◽  
Hai-Hua Huang ◽  
Huiping Liu ◽  
Zhuofeng Ke ◽  
Wen-Wen Yong ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Photocatalytic Performance ◽  
Aqueous Media ◽  
Highly Efficient ◽  
Co Conversion

A Co-based complex displayed the highest photocatalytic performance for CO2 to CO conversion in aqueous media.

Tuning the pore structures and photocatalytic properties of a 2D covalent organic framework with multi-branched photoactive moieties

Nanoscale ◽  
2020 ◽  
Vol 12 (30) ◽  
pp. 16136-16142
Author(s):  
Xuan Wang ◽  
Ming-Jie Dong ◽  
Chuan-De Wu
Keyword(s):  
Photocatalytic Properties ◽  
Effective Strategy ◽  
Covalent Organic Framework ◽  
Highly Efficient ◽  
Pore Structures ◽  
Local Connection ◽  
Organic Framework

An effective strategy to incorporate accessible metalloporphyrin photoactive sites into 2D COFs by establishing a 3D local connection for highly efficient photocatalysis was developed.

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