Escherichia coli-based cell free production of flagellin and ordered flagellin display on virus-like particles

2013 ◽  
Vol 110 (8) ◽  
pp. 2073-2085 ◽  
Author(s):  
Yuan Lu ◽  
John P. Welsh ◽  
Wei Chan ◽  
James R. Swartz
Keyword(s):  
Escherichia Coli ◽  
Virus Like Particles

Human papillomavirus L1 protein expressed in Escherichia coli self-assembles into virus-like particles that are highly immunogenic

2016 ◽  
Vol 220 ◽  
pp. 97-103 ◽  
Author(s):  
Yumei Chen ◽  
Yunchao Liu ◽  
Gaiping Zhang ◽  
Aiping Wang ◽  
Ziming Dong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Papillomavirus ◽  
Virus Like Particles ◽  
L1 Protein

scholarly journals A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes

1970 ◽  
Vol 117 (1) ◽  
pp. 101-118 ◽  
Author(s):  
R. A. Cox
Keyword(s):  
Escherichia Coli ◽  
Ionic Strength ◽  
Secondary Structure ◽  
Ribonucleic Acid ◽  
Spectrophotometric Study ◽  
Urea Concentration ◽  
Model Systems ◽  
Base Pairs ◽  
Virus Like Particles ◽  
Rabbit Reticulocytes

The spectrum of RNA from the smaller and larger subparticles of rabbit reticulocyte ribosomes was studied as a function of pH, ionic strength, urea concentration and temperature. It was inferred that both RNA species form short double-helical segments of not more than about 10 base-pairs in length. Not more than about 70% of the base residues may be located in double-helical segments. RNA from the larger subparticle is richer in guanine and cytosine residues and its secondary structure is the more stable. These conclusions are based on the use of double-helical RNA from virus-like particles and of unfractionated Escherichia coli tRNA as model systems.

scholarly journals Production of SARS-CoV-2 virus-like particles in insect cells

2021 ◽  
Author(s):  
Youjun Mi ◽  
Tao Xie ◽  
Bingdong Zhu ◽  
Jiying Tan ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Health ◽  
Insect Cells ◽  
Vaccine Development ◽  
Recombinant Baculovirus ◽  
Expression Plasmid ◽  
Virus Like Particles ◽  
Recombinant Bacmid ◽  
Multiple Cloning Sites ◽  
S Genes

ABSTRACTCoronavirus disease (COVID-19) causes a serious threat to human health. To production of SARS-COV-2 virus-like particles (VLPs) in insect cells for vaccine development and scientific research. The E, M and S genes were cloned into multiple cloning sites of the new triple expression plasmid with one p10 promoter, two pPH promoters and three multiple cloning sites. The plasmid was transformed into DH10 BacTMEscherichia coli competent cells to obtain recombinant bacmid. Then the recombinant bacmid was transfected in ExpiSf9™ insect cells to generate recombinant baculovirus. After ExpiSf9™ infected with the recombinant baculovirus, the E, M, and S protein co-expressed in insect cells. Finally, SARS-CoV-2 VLPs were self-assembled in insect cells after infection. The morphology and the size of SARS-CoV-2 VLPs are similar to the native virions.

High Level Production of Hybrid Poty virus–like Particles Carrying Repetitive Copies of Foreign Antigens in Escherichia coli

1993 ◽  
Vol 11 (10) ◽  
pp. 1166-1170 ◽  
Author(s):  
Mittur N. Jagadish ◽  
Ross C. Hamilton ◽  
Caroline S. Fernandez ◽  
Peter Schoofs ◽  
Kathy M. Davern ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virus Like Particles ◽  
High Level ◽  
Level Production

scholarly journals Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response

2001 ◽  
Vol 75 (22) ◽  
pp. 11010-11016 ◽  
Author(s):  
Catherine Fromantin ◽  
Béatrice Jamot ◽  
Jean Cohen ◽  
Lionel Piroth ◽  
Pierre Pothier ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lymph Node ◽  
Antibody Response ◽  
Cholera Toxin ◽  
Lymphoid Tissues ◽  
Intranasal Immunization ◽  
Virus Like Particles ◽  
Heat Labile ◽  
Lymph Node Cells

ABSTRACT We investigated the rotavirus-specific lymphocyte responses induced by intranasal immunization of adult BALB/c mice with rotavirus 2/6 virus-like particles (2/6-VLPs) of the bovine RF strain, by assessing the profile of cytokines produced after in vitro restimulation and serum and fecal antibody responses. The cytokines produced by splenic cells were first evaluated. Intranasal immunization with 50 μg of 2/6-VLPs induced a high serum antibody response, including immunoglobulin G1 (IgG1) and IgG2a, a weak fecal antibody response, and a mixed Th1/Th2-like profile of cytokines characterized by gamma interferon and interleukin 10 (IL-10) production and very low levels of IL-2, IL-4, and IL-5. Intranasal immunization with 10 μg of 2/6-VLPs coadministered with the mucosal adjuvants cholera toxin andEscherichia coli heat-labile toxin (LT) considerably enhanced the Th1/Th2-like response; notably, significant levels of IL-2, IL-4, and IL-5 were observed. Since rotavirus is an enteric pathogen, we next investigated the production of IL-2 and IL-5, as being representative of Th1 and Th2 responses, by Peyer's patch and mesenteric lymph node cells from mice immunized intranasally with 2/6-VLPs and LT. The results were compared to those obtained from splenic and cervical lymph node cells. We found that both cytokines were produced by cells from each of these lymphoid tissues. These results confirm the Th1/Th2-like response observed at the systemic level and show, on the assumption that T cells are the primary cells producing the cytokines after in vitro restimulation, that rotavirus-specific T lymphocytes are present in the intestine after intranasal immunization with 2/6-VLPs and LT.

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