Quantitating intraparticle O2gradients in solid supported enzyme immobilizates: Experimental determination of their role in limiting the catalytic effectiveness of immobilized glucose oxidase

2013 ◽  
Vol 110 (8) ◽  
pp. 2086-2095 ◽  
Author(s):  
Juan M. Bolivar ◽  
Tanja Consolati ◽  
Torsten Mayr ◽  
Bernd Nidetzky
Keyword(s):  
Experimental Determination ◽  
Glucose Oxidase ◽  
Immobilized Glucose Oxidase

Enzyme thermistor determination of glucose in serum using immobilized glucose oxidase

1977 ◽  
Vol 81 (2) ◽  
pp. 163-175 ◽  
Author(s):  
B. Danielsson ◽  
Kerstin Gadd ◽  
B. Mattiasson ◽  
K. Mosbach
Keyword(s):  
Glucose Oxidase ◽  
Immobilized Glucose Oxidase ◽  
Enzyme Thermistor

Determination of glucose in human serum based on an onion primary cuticula biosensor immobilized glucose oxidase

2012 ◽  
Vol 4 (5) ◽  
pp. 1432 ◽  
Author(s):  
Wenjuan Jia ◽  
Wenjuan Liu ◽  
Yan Zhang ◽  
Miao Cui ◽  
Shaomin Shuang ◽  
...  
Keyword(s):  
Human Serum ◽  
Glucose Oxidase ◽  
Immobilized Glucose Oxidase

Determination of the inherent kinetics of immobilized glucose oxidase using a diffusion cell

1996 ◽  
Vol 15 (2) ◽  
pp. 87 ◽  
Author(s):  
S. A. M. van Stroe-Biezen ◽  
J. M. H. van der Loo ◽  
L. J. J. Janssen ◽  
F. M. Everaerts
Keyword(s):  
Glucose Oxidase ◽  
Diffusion Cell ◽  
Immobilized Glucose Oxidase ◽  
Kinetics Of

Immobilized glucose oxidase used in the continuous-flow determination of serum glucose.

1976 ◽  
Vol 22 (7) ◽  
pp. 1017-1023 ◽  
Author(s):  
L P León ◽  
S Narayanan ◽  
R Dellenbach ◽  
C Horvath
Keyword(s):  
Glucose Oxidase ◽  
Continuous Flow ◽  
Serum Glucose ◽  
Long Term Storage ◽  
Free Glucose ◽  
Immobilized Glucose Oxidase ◽  
Analytical System ◽  
Single Enzyme

Abstract We used a tubular glucose-oxidase wall reactor in the "AutoAnalyzer II" continuous-flow analytical system to determine glucose in blood serum. Sensitivity was high and wash characteristics were satisfactory with use of a 30-cm tube containing immobilized glucose oxidase. Results compared favorably with those of the conventional free-enzyme method. More than 25000 such assays can be performed with a single enzyme tube, which also shows long-term storage stability. Because of the steady-state chemistry 60 samples can be analyzed per hour. The linearity of the method is excellent and sample interaction from 5.0 to 1.0 g/liter is less than 5%. Results correlate well (greater than 0.993) with those obtained with both the neocuproine method used in the "SMA 12/60" multichannel analytical system and the free glucose oxidase method used in the AutoAnalyzer.

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