Multi-enzymatic one-pot reduction of dehydrocholic acid to 12-keto-ursodeoxycholic acid with whole-cell biocatalysts

2012 ◽  
Vol 110 (1) ◽  
pp. 68-77 ◽  
Author(s):  
Boqiao Sun ◽  
Christina Kantzow ◽  
Sven Bresch ◽  
Kathrin Castiglione ◽  
Dirk Weuster-Botz
Keyword(s):  
Ursodeoxycholic Acid ◽  
One Pot ◽  
Whole Cell ◽  
Dehydrocholic Acid

Enantiocomplementary decarboxylative hydroxylation combining photocatalysis and whole-cell biocatalysis in a one-pot cascade process

2019 ◽  
Vol 21 (8) ◽  
pp. 1907-1911 ◽  
Author(s):  
Jian Xu ◽  
Mamatjan Arkin ◽  
Yongzhen Peng ◽  
Weihua Xu ◽  
Huilei Yu ◽  
...  
Keyword(s):  
Cascade Process ◽  
One Pot ◽  
Whole Cell ◽  
Whole Cell Biocatalysis

The first demonstration of photochemo-enzymatic whole-cell one-pot enantiocomplementary decarboxylative hydroxylation.

Whole-Cell Cascade Biotransformations for One-Pot Multistep Organic Synthesis

ChemCatChem ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 2164-2178 ◽  
Author(s):  
Shuke Wu ◽  
Zhi Li
Keyword(s):  
Organic Synthesis ◽  
One Pot ◽  
Whole Cell

Whole-Cell One-Pot Biosynthesis of Azelaic Acid

ChemCatChem ◽  
2013 ◽  
Vol 6 (4) ◽  
pp. 1003-1009 ◽  
Author(s):  
Konrad B. Otte ◽  
Jens Kittelberger ◽  
Marko Kirtz ◽  
Bettina M. Nestl ◽  
Bernhard Hauer
Keyword(s):  
Azelaic Acid ◽  
One Pot ◽  
Whole Cell

scholarly journals Whole-Cell One-Pot Biosynthesis of Azelaic Acid

ChemCatChem ◽  
2014 ◽  
Vol 6 (4) ◽  
pp. 899-899
Author(s):  
Konrad B. Otte ◽  
Jens Kittelberger ◽  
Marko Kirtz ◽  
Bettina M. Nestl ◽  
Bernhard Hauer
Keyword(s):  
Azelaic Acid ◽  
One Pot ◽  
Whole Cell

scholarly journals Chemo-biocatalytic one-pot two-step conversion of cyclic amine to lactam using whole cell monoamine oxidase

2016 ◽  
Vol 92 (7) ◽  
pp. 1558-1565 ◽  
Author(s):  
Petra Zajkoska ◽  
Max Cárdenas-Fernández ◽  
Gary J Lye ◽  
Michal Rosenberg ◽  
Nicholas J Turner ◽  
...  
Keyword(s):  
Monoamine Oxidase ◽  
Cyclic Amine ◽  
One Pot ◽  
Whole Cell

scholarly journals Efficient Production of 3′-Sialyllactose by Single Whole-Cell in One-Pot Biosynthesis

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 932
Author(s):  
Zhongkui Li ◽  
Xiangsong Chen ◽  
Zhijian Ni ◽  
Lixia Yuan ◽  
Lijie Sun ◽  
...  
Keyword(s):  
Surfactant Concentration ◽  
Enzyme System ◽  
Efficient Production ◽  
Optimal Conditions ◽  
One Pot ◽  
Whole Cell ◽  
Economic Competitiveness ◽  
Enzyme Cascade ◽  
L System ◽  
Conversion Temperature

Sialyllactose (SL) is one of the most important acidic oligosaccharides in human milk, which plays an important role in the health of infants. In this work, an efficient multi-enzyme cascade was developed in a single whole cell to produce 3′-SL. We constructed two compatible plasmids with double cloning sites to co-express four genes. Different combinations were assessed to verify the optimal catalytic ability. Then, the conversion temperature, pH, and stability under the optimal temperature and pH were investigated. Moreover, the optimal conversion conditions and surfactant concentration were determined. By using the optimal conditions (35 °C, pH 7.0, 20 mM polyphosphate, 10 mM cytidine monophosphate (CMP), 20 mM MgCl2), 25 mL and 4 L conversion systems were carried out to produce 3′-SL. Similar results were obtained between different volume conversion reactions, which led the maximum production of 3′-SL to reach 53 mM from 54.2 mM of sialic acid (SA) in the 25 mL system and 52.8 mM of 3′-SL from 53.8 mM of SA in the 4 L system. These encouraging results demonstrate that the developed single whole-cell multi-enzyme system exhibits great potential and economic competitiveness for the manufacture of 3′-SL.

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