Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification

2012 ◽  
Vol 109 (10) ◽  
pp. 2589-2598 ◽  
Author(s):  
Yan Brodsky ◽  
Cheng Zhang ◽  
Yinges Yigzaw ◽  
Ganesh Vedantham
Keyword(s):  
Monoclonal Antibody ◽  
Caprylic Acid ◽  
Acid Precipitation ◽  
Precipitation Method ◽  
Antibody Purification

Purification of IgG monoclonal antibody by caprylic acid precipitation

1983 ◽  
Vol 65 (1-2) ◽  
pp. 269-271 ◽  
Author(s):  
C. Russo ◽  
L. Callegaro ◽  
E. Lanza ◽  
S. Ferrone
Keyword(s):  
Monoclonal Antibody ◽  
Caprylic Acid ◽  
Acid Precipitation

Multiple functions of caprylic acid-induced impurity precipitation for process intensification in monoclonal antibody purification

2018 ◽  
Vol 279 ◽  
pp. 13-21 ◽  
Author(s):  
Anja Trapp ◽  
Alexander Faude ◽  
Natalie Hörold ◽  
Sven Schubert ◽  
Sabine Faust ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Caprylic Acid ◽  
Process Intensification ◽  
Multiple Functions ◽  
Antibody Purification

Preparation of Amorphous Nanosilica from Philippine Waste Rice Hull via Acid Precipitation Method

2016 ◽  
Vol 864 ◽  
pp. 112-116
Author(s):  
Rinlee Butch M. Cervera ◽  
Emie A. Salamangkit-Mirasol
Keyword(s):  
Electron Microscopy ◽  
Low Cost ◽  
Agricultural Waste ◽  
Acid Precipitation ◽  
Particle Size Analysis ◽  
Precipitation Method ◽  
Rice Hull ◽  
Size Analysis ◽  
Rice Hull Ash ◽  
Transmission Electron

Rice hull or rice husk (RH) is an agricultural waste obtained from milling rice grains. Since RH has no commercial value and is difficult to use in agriculture, its volume is often reduced through open field burning which is an environmental hazard. In this study, amorphous nanosilica from Philippine waste RH was prepared via acid precipitation method. The synthesized samples were fully characterized for its microstructural properties. X-ray diffraction pattern reveals that the structure of the prepared sample is amorphous in nature while Fourier transform infrared spectrum showed the different vibration bands of the synthesized sample. Scanning electron microscopy (SEM) and particle size analysis (PSA) confirmed the presence of agglomerated silica particles. On the other hand, transmission electron microscopy (TEM) revealed an amorphous sample with grain sizes of about 5 to 20 nanometer range and has about 95 % purity according to EDS analyses. The elemental mapping also suggests that leaching of rice hull ash effectively removed the metallic impurity such as potassium element in the material. Hence, amorphous nanosilica was successfully prepared via a low-cost acid precipitation method from Philippine waste rice hull.

A safe, effective, and facility compatible cleaning in place procedure for affinity resin in large-scale monoclonal antibody purification

2013 ◽  
Vol 1308 ◽  
pp. 86-95 ◽  
Author(s):  
Lu Wang ◽  
Jill Dembecki ◽  
Neil E. Jaffe ◽  
Brian W. O’Mara ◽  
Hui Cai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Large Scale ◽  
Antibody Purification ◽  
Affinity Resin

Integrated Polishing Steps for Monoclonal Antibody Purification

2009 ◽  
pp. 145-167 ◽  
Author(s):  
Sanchayita Ghose ◽  
Mi Jin ◽  
Jia Liu ◽  
John Hickey
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Purification

INTEGRATED POLISHING STEPS FOR MONOCLONAL ANTIBODY PURIFICATION

2017 ◽  
pp. 303-323
Author(s):  
Sanchayita Ghose ◽  
Mi Jin ◽  
Jia Liu ◽  
John Hickey ◽  
Steven Lee
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Purification
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