Using FTIR spectroscopy to model alkaline pretreatment and enzymatic saccharification of six lignocellulosic biomasses

2011 ◽  
Vol 109 (4) ◽  
pp. 894-903 ◽  
Author(s):  
Deborah L. Sills ◽  
James M. Gossett
Keyword(s):  
Ftir Spectroscopy ◽  
Enzymatic Saccharification ◽  
Alkaline Pretreatment

Microwave assisted alkaline pretreatment to enhance enzymatic saccharification of catalpa sawdust

2016 ◽  
Vol 221 ◽  
pp. 26-30 ◽  
Author(s):  
Shuguang Jin ◽  
Guangming Zhang ◽  
Panyue Zhang ◽  
Fan Li ◽  
Siqi Wang ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Alkaline Pretreatment ◽  
Microwave Assisted

Recycling of Black Liquor Generated from Cellulosic Ethanol Process for Plant Growth

2020 ◽  
Vol 14 (4) ◽  
pp. 511-516
Author(s):  
Changzhong Song ◽  
Bowen Zhang ◽  
Wen Wang ◽  
Xuesong Tan ◽  
Zahoor ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Black Liquor ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Alkaline Pretreatment ◽  
Solid Loading ◽  
Solid Ratio ◽  
Saccharification Efficiency

The alkaline pretreatment has the advantages of low energy input and atmospheric pressure to highly enhance the conversion of lignocellulose to ethanol. However, the black liquor from the process would pollute the environment, which hinders its industrial application. This study selected the potassium hydroxide (KOH) as the alkaline reagent for lignocellulosic pretreatment and investigated the feasibility of recycling the black liquor (BL) as molecular bio-activator for plant growth. After optimization of KOH pretreatment, the enzymatic saccharification efficiency of rice straw achieved to 86.6% under the optimum condition of 2% KOH, 15:1 of liquid–solid ratio, 70 °C for 1 h. The ethanol production and conversion ratio was 32.24 g/L and 53.0% respectively at 20% solid loading. The tobacco cultured in the nutrient solution with BL was more luxuriant than that without BL, of which the dry weight of plant increased 367% and the leaf area increment of tobacco was about 2∼4 times than the control after 30 days. Thus this study provided a promising way to accelerate the industrialization of alkaline pretreatment for cellulosic ethanol production.

scholarly journals Alkaline pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for ethanol production 1) Pengolahan awal dengan basa NaOH dan sakarifikasi enzimatis serat tandan kosong kelapa sawit (TKKS) untuk produksi etanol

2016 ◽  
Vol 78 (2) ◽  
Author(s):  
Yanni SUDIYANI ◽  
Kiky C SEMBIRING ◽  
Hendris HENDARSYAH ◽  
Syarifah ALAWIYAH
Keyword(s):  
Ethanol Production ◽  
Palm Oil ◽  
Oil Palm ◽  
Hydrolytic Enzymes ◽  
Enzymatic Saccharification ◽  
Reaction Times ◽  
Alkaline Pretreatment ◽  
Empty Fruit Bunch ◽  
Alkali Pretreatment ◽  
Pretreatment Condition

Abstract Alkaline pretreatment of oil palm empty fruit bunch (EFB) fiber was conducted to improve enzymatic sacchari-fication of EFB fiber for ethanol production.  EFB as one of the major biomass wastes from palm oil industry is a complex lignocellulosic material consists of 41.3 – 46.5% of cellulose, 25.3 – 33.8% of hemicellulose and 27.6 – 32.5% of lignin.  Alkali pretreatment of EFB using NaOH 1 N with temperature at 30 and 600C and reaction times of 30, 60, 90, 120 and 150 minutes were investigated.  Furthermore, the enzymatic saccharification of pretreated EFB was examined. The pretreated substrate was subjected to an enzymatic saccharification using meicelase (10, 20 and 40 FPU/g substrate) at 400C, pH 4.5, 100 rpm for conversion of cellulose and hemicellulose in palm oil EFB to monomeric sugars. The alkali pretreatment of EFB using NaOH can significantly improve the enzymatic saccharification of EFB by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes.  The results showed that the optimum pretreatment condition was NaOH 1 N at 300C and 90 minutes with the optimum component loss of lignin and hemicellulose was 45.8  % and 35.6  % respectively.  The saccharification of EFB pretreated by NaOH 1 N (at 300C and 90 minutes) for 45 hours and pH 4.5 resulted in optimum saccharification of 63.8 %.  Abstrak Pengolahan awal (pretreatment) serat tandan kosong kelapa sawit (TKKS) dengan basa NaOH telah dilakukan untuk meningkatkan sakarifikasi enzimatik TKKS menjadi etanol.  TKKS merupakan bahan lignoselulosa yang terdiri dari selulosa 41,3– 46,%,  hemicellulosa 25,3 – 33,8% dan lignin 27,6 – 32,5%. Pretreatment TKKS dilakukan dengan NaOH 1 N dengan variasi suhu 300 dan 600C dan variasi waktu 30, 60, 90, 120 dan 150 menit.  Konversi selulosa dan hemiselulosa hasil pretreatment TKKS menjadi gula dilaku-kan dengan sakarifikasi enzimatik menggunakan enzim meiselase (10, 20 dan 40 FPU/g substrat) pada suhu 400C, pH 4,5 dengan shaker 100 rpm.  Pretretament TKKS dengan basa   NaOH   dapat   meningkatkan   sakarifikasi enzimatik dengan berkurangnya lignin dan hemiselulosa secara signifikan dan memudahkan masuknya enzim hidrolitik.  Hasil pretreatment dengan NaOH 1N pada suhu 300C dan 90 menit menunjukkan kondisi optimum untuk penghilangan lignin dan hemiselulosa berturut-turut sebesar 45,8  % and 35,6  %.  Hasil sakarifikasi optimum yaitu 63,8 % dicapai setelah 45 jam sakarifisi pada pH 4,5. 

scholarly journals Understanding of alkaline pretreatment parameters for corn stover enzymatic saccharification

2013 ◽  
Vol 6 (1) ◽  
pp. 8 ◽  
Author(s):  
Ye Chen ◽  
Mark A Stevens ◽  
Yongming Zhu ◽  
Jason Holmes ◽  
Hui Xu
Keyword(s):  
Corn Stover ◽  
Enzymatic Saccharification ◽  
Alkaline Pretreatment

scholarly journals ENZYMATIC SACHARIFICATION OF ALKALINE PRETREATED RICE STRAW BY CELLULASE FROM CELLULOSIMICROBIUM SP. MP1

2021 ◽  
Vol 58 (6A) ◽  
pp. 244
Author(s):  
Dao Thi Thanh Xuan ◽  
Le Thanh Ha ◽  
Phi Quyet Tien
Keyword(s):  
Technological Parameter ◽  
Enzymatic Saccharification ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Alkaline Pretreatment ◽  
Cellulose Content ◽  
Liquid Ratio ◽  
Hydrolysis Time ◽  
Saccharification Yield ◽  
Hydrolysis Conditions

The effects of different physical and technological parameter such as time, substrate to liquid ratio, enzyme concentration, temperature, and pH on enzymatic saccharification of alkaline pretreated straw cellulose were studied. For alkaline pretreatment, the straw was incubated with 10 % NaOH at ratio 1:20 (w/v) at 90 °C for 1 hour. After the alkaline pretreatment the cellulose content increased from 50.2 % (w/w) to 67.3 % (w/w). Enzyme used for saccharification of treated and untreated straw  was produced from Cellulosimicrobium sp MP1 which was isolated from termite gut. Results from research showed that the highest percentage of saccharification of alkaline pretreated straw was 69.91 %, corresponding to 10.58 mg/mL of reducing sugar. The hydrolysis conditions for reaching this highest saccharification yield were: temperature of 55 ºC, substrate to liquid ratio of 2 g/100 mL, enzyme concentration of 37.5 U/g, pH of 5.5 and hydrolysis time of 48 hours.

Acid-Alkaline Two-Stage Pretreatments of Corn Stover for Enhancing Enzymatic Saccharification

2013 ◽  
Vol 724-725 ◽  
pp. 207-211 ◽  
Author(s):  
Hai Song Wang ◽  
Hong Ling Gao ◽  
Bin Li ◽  
Xin Dong Mu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Lignocellulosic Biomass ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Alkaline Pretreatment ◽  
Two Stage ◽  
Glucose Yield ◽  
Second Stage

For the enzymatic saccharification of lignocellulosic biomass, single acid or alkaline pretreatment is not satisfactory because of the low sugar yields together with the neutralization of residual chemicals before enzymatic hydrolysis. Herein, an acid-alkaline two-stage pretreatment process was designed to treat corn stover. During the process, the pretreated liquid from the first stage and the solid residues from the second stage were mixed together for the subsequent simultaneous enzymatic hydrolysis, where a mixture of cellulase with an activity loading of 20 FPU/g substrate, cellobiase with an activity loading of 5 U/g substrate, and xylanase with an activity loading of 200 U/g substrate was used. Compared to the single acid or alkaline pretreatment, the acid-alkaline two-stage pretreatment could significantly improve the enzymatic saccharification, and 91.2% glucose yield with 52.56% of the theoretical total reducing sugar yield was achieved after the subsequent enzymatic hydrolysis.

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