Collisional fragmentation of central carbon metabolites in LC-MS/MS increases precision of 13C metabolic flux analysis

2011 ◽  
Vol 109 (3) ◽  
pp. 763-771 ◽  
Author(s):  
Martin Rühl ◽  
Beat Rupp ◽  
Katharina Nöh ◽  
Wolfgang Wiechert ◽  
Uwe Sauer ◽  
...  
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
13C Metabolic Flux Analysis ◽  
Central Carbon

scholarly journals Metabolic mechanism of Saccharomyces cerevisiae under different physiological conditions based on non-stationary 13C metabolic flux analysis

2020 ◽  
Author(s):  
Huan Li ◽  
Min Chen ◽  
Peng Liu ◽  
Shuai Wang ◽  
JY Xia
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Steady State ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Tricarboxylic Acid Cycle ◽  
Energy Charge ◽  
Flux Analysis ◽  
13C Metabolic Flux Analysis ◽  
Metabolic Flux Distribution ◽  
Central Carbon

Abstract Crabtree effect is well known for Saccharomyces cerevisiae, and is defined as glucose-induced repression of respiratory flux. Even though a number of hypotheses have been formulated, its triggering mechanisms are still unknown. At present, the information about intracellular metabolic flux can be obtained by the 13C isotope labeling experiments. 13C metabolic flux analysis(13C-MFA) is a traditional method for calculating metabolic flux based on isotopic steady state. Another new method (INST-13C-MFA: Isotopically nonstationary metabolic flux analysis) based on isotope non-steady state is being used by researchers. In this review, we have chemostatized S. cerevisiae at three different dilution rates (D=0.12, 0.22, 0.32 h-1) and obtained the metabolic flux distribution of the intracellular central carbon metabolic of S. cerevisiae using INST-13C-MFA. Combined with the metabolome and metabolic fluxome data, we found obvious metabolic flux shift under the three different physiological states. In this process, pyruvate decarboxylase, ethanol dehydrogenase and acetyl-CoA synthase(AcCoA) catalyzed reactions were key points. Negative correlation between relative flux of embden meyerh of pathway(EMP) and tricarboxylic acid cycle(TCA) and biomass yield, while positive correlation for pentose phosphate pathway(PPP) were observed. Yield of acetate and glycerol did not change significantly, while that of ethanol increased sharply. In the central carbon metabolism (CCM), most of the carbon flux (70%) was directed to the EMP. At the same time, the energy charge increased with dilution rate, and the cell's energy supply mode gradually shifted from oxidative respiration to substrate level phosphorylation mode.

scholarly journals Mapping Salmonella typhimurium pathways using 13C metabolic flux analysis

2019 ◽  
Vol 52 ◽  
pp. 303-314 ◽  
Author(s):  
Daniela M. Correia ◽  
Cintia R. Sargo ◽  
Adilson J. Silva ◽  
Sophia T. Santos ◽  
Roberto C. Giordano ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
13C Metabolic Flux Analysis
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