The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

2011 ◽  
Vol 108 (9) ◽  
pp. 2141-2150 ◽  
Author(s):  
Mattia Matasci ◽  
Lucia Baldi ◽  
David L. Hacker ◽  
Florian M. Wurm
Keyword(s):  
Cell Line ◽  
Stable Cell Line ◽  
Piggybac Transposon ◽  
Stable Cell Line Generation ◽  
Cell Line Generation ◽  
Line Generation

scholarly journals Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0194887 ◽  
Author(s):  
Roman J. Szczesny ◽  
Katarzyna Kowalska ◽  
Kamila Klosowska-Kosicka ◽  
Aleksander Chlebowski ◽  
Ewelina P. Owczarek ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cell Line ◽  
Stable Cell Line ◽  
Recombination System ◽  
Stable Cell Line Generation ◽  
Human Proteins ◽  
Cell Line Generation ◽  
Line Generation

scholarly journals Recombinant antibody mixtures; optimization of cell line generation and single-batch manufacturing processes

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Søren K Rasmussen ◽  
Lars S Nielsen ◽  
Christian Müller ◽  
Thomas Bouquin ◽  
Henrik Næsted ◽  
...  
Keyword(s):  
Cell Line ◽  
Recombinant Antibody ◽  
Manufacturing Processes ◽  
Single Batch ◽  
Cell Line Generation ◽  
Line Generation

scholarly journals Sensitization of breast cancer cells to doxorubicin via stable cell line generation and overexpression of DFF40

2015 ◽  
Vol 93 (6) ◽  
pp. 604-610 ◽  
Author(s):  
Fatemeh Bagheri ◽  
Shahrokh Safarian ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Nader Sheibani
Keyword(s):  
Cell Cycle ◽  
Annexin V ◽  
Eukaryotic Expression ◽  
Stable Cell Line ◽  
Cell Cycle Distribution ◽  
Distribution Analysis ◽  
Transfected Cells ◽  
Dna Laddering ◽  
Stable Cell Line Generation ◽  
Cell Line Generation

There are a number of reports demonstrating a relationship between the alterations in DFF40 expression and development of some cancers. Here, increased DFF40 expression in T-47D cells in the presence of doxorubicin was envisaged for therapeutic usage. The T-47D cells were transfected with an eukaryotic expression vector encoding the DFF40 cDNA. Following incubation with doxorubicin, propidium iodide (PI) staining was used for cell cycle distribution analysis. The rates of apoptosis were determined by annexin V/PI staining. Apoptosis was also evaluated using the DNA laddering analysis. The viability of DFF40-transfected cells incubated with doxorubicin was significantly decreased compared with control cells. However, there were no substantial changes in the cell cycle distribution of pIRES2-DFF40 cells incubated with doxorubicin compared to control cells. The expression of DFF40, without doxorubicin incubation, had also no significant effect on the cell cycle distribution. There was no DNA laddering in cells transfected with the empty pIRES2 vector when incubated with doxorubicin. In contrast, DNA laddering was observed in DFF40 transfected cells in the presence of doxorubicin after 48 h. Also, the expression of DFF40 and DFF45 was increased in DFF40 transfected cells in the presence of doxorubicin enhancing cell death. Collectively our results indicated that co-treatment of DFF40-transfected cells with doxorubicin can enhance the killing of these tumor cells via apoptosis. Thus, modulation of DFF40 level may be a beneficial strategy for treatment of chemo-resistant cancers.

scholarly journals QOLP-35. PEDIATRIC POST-MORTEM TISSUE DONATION DURING A PANDEMIC: A MODEL OF COLLABORATION

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi190-vi191
Author(s):  
Esteban Uceda ◽  
Cindy Campbell ◽  
Sheila McThenia ◽  
Melanie Degliuomini ◽  
Yasmin Khakoo ◽  
...  
Keyword(s):  
Cell Line ◽  
Tissue Donation ◽  
Time Of Death ◽  
Post Mortem ◽  
Patient Demographics ◽  
Genomic Analyses ◽  
Rapid Autopsy ◽  
Positive Effect ◽  
Cell Line Generation ◽  
Line Generation

Abstract BACKGROUND Obtaining post-mortem tissue from pediatric oncology patients is not only critical to research, but studies show that participating in the process can also help grieving families heal. Since 2019, the national Gift from a Child program — a multi-institutional effort to increase the rate of rapid autopsies for pediatric CNS tumor patients — has made significant progress. Collecting high-quality post-mortem tissue has advanced research through cell line generation and genomic analyses. Unfortunately, some autopsy programs temporarily shut down during the COVID-19 pandemic. METHODS We retrospectively reviewed autopsies of four patients treated at Memorial Sloan Kettering (MSK) who underwent limited brain post-mortem examination at Weill Cornell Medicine College (WCMC) from June 2020 to June 2021. We collected patient demographics; DNR status; time of death and procedure; restrictions due to the COVID-19 pandemic; and results of the tissue analysis. Each case presented unique challenges and the timing of securing parental consent varied. RESULTS Three of four specimens were processed within 12 hours of the time of death. Two Spanish-speaking families required interpreters services to obtain consent. In all cases tumor aliquots were flash frozen for further study. All specimens contained viable tumor and cell line generation was successful in one case. All families/caregivers expressed gratitude for the opportunity to participate and for the handling of the procedures. DISCUSSION Despite the sensitive nature of these cases, clinicians should offer the option of a rapid autopsy to caregivers of pediatric patients based on the scientific need and the positive effect it has on grieving families. This paper outlines the logistical efforts required for these donations to take place and provides a framework for providers to offer rapid autopsy as an option for families through this program.

Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells

2011 ◽  
Vol 109 (4) ◽  
pp. 1007-1015 ◽  
Author(s):  
Lianchun Fan ◽  
Ibrahim Kadura ◽  
Lara E. Krebs ◽  
Christopher C. Hatfield ◽  
Margaret M. Shaw ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Cell Line ◽  
Gene Knockout ◽  
Cho Cell ◽  
Glutamine Synthetase Gene ◽  
Cho Cell Line ◽  
Cell Line Generation ◽  
Line Generation

Development of a highly-efficient CHO cell line generation system with engineered SV40E promoter

2013 ◽  
Vol 168 (4) ◽  
pp. 652-658 ◽  
Author(s):  
Lianchun Fan ◽  
Ibrahim Kadura ◽  
Lara E. Krebs ◽  
Jeffery L. Larson ◽  
Daniel M. Bowden ◽  
...  
Keyword(s):  
Cell Line ◽  
Generation System ◽  
Cho Cell ◽  
Highly Efficient ◽  
Cho Cell Line ◽  
Cell Line Generation ◽  
Line Generation

CRISPR/Ca12a‐mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction

2020 ◽  
pp. 2000308
Author(s):  
Patrick G. Schweickert ◽  
Ning Wang ◽  
Stephanie L. Sandefur ◽  
Michael E. Lloyd ◽  
Stephen F. Konieczny ◽  
...  
Keyword(s):  
Cell Line ◽  
Genome Engineering ◽  
Generation Process ◽  
Cell Line Generation ◽  
Multiple Stages ◽  
Line Generation
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