Purification of singly PEGylated α-lactalbumin using charged ultrafiltration membranes

2010 ◽  
Vol 108 (4) ◽  
pp. 822-829 ◽  
Author(s):  
Krisada Ruanjaikaen ◽  
Andrew L. Zydney
Keyword(s):  
Ultrafiltration Membranes ◽  
Charged Ultrafiltration Membranes

scholarly journals Fractionation of Glycomacropeptide from Whey Using Positively Charged Ultrafiltration Membranes

Foods ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 166 ◽  
Author(s):  
Abhiram Arunkumar ◽  
Mark Etzel
Keyword(s):  
Polymer Brushes ◽  
Cheese Whey ◽  
Fold Increase ◽  
Membrane System ◽  
Attractive Alternative ◽  
Salt Tolerant ◽  
Water Addition ◽  
Ultrafiltration Membranes ◽  
Charged Ultrafiltration Membranes ◽  
Positively Charged

Fractionation of the bovine glycomacropeptide (GMP) from the other proteins in cheese whey was examined using ultrafiltration membranes surface modified to contain positively charged polymer brushes made of polyhexamethylene biguanide. By placing a strong positive charge on a 1000 kDa ultrafiltration membrane and adjusting the pH of whey close to the isoelectric point of GMP, a 14-fold increase in selectivity was observed compared to unmodified membranes. A one stage membrane system gave 90% pure GMP and a three-stage rectification system gave 97% pure GMP. The charged membrane was salt-tolerant up to 40 mS cm−1 conductivity, allowing fractionation of GMP directly from cheese whey without first lowering the whey conductivity by water dilution. Thus, similarly sized proteins that differed somewhat in isoelectric points and were 50–100 fold smaller than the membrane molecular weight cut-off (MWCO), were cleanly fractionated using charged ultrafiltration membranes without water addition. This is the first study to report on the use of salt-tolerant charged ultrafiltration membranes to produce chromatographically pure protein fractions from whey, making ultrafiltration an attractive alternative to chromatography for dairy protein fractionation.

Use of Negatively-Charged Ultrafiltration Membranes

1980 ◽  
pp. 605-608 ◽  
Author(s):  
D. Bhattacharyya ◽  
M. G. Balko ◽  
C. Cheng ◽  
S. E. Gentry
Keyword(s):  
Ultrafiltration Membranes ◽  
Charged Ultrafiltration Membranes

Plasmid DNA transmission through charged ultrafiltration membranes

2009 ◽  
Vol 344 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
Kimberly Ager ◽  
David R. Latulippe ◽  
Andrew L. Zydney
Keyword(s):  
Plasmid Dna ◽  
Ultrafiltration Membranes ◽  
Charged Ultrafiltration Membranes

Electrostatic Sieve Separation of Dextransulfate and Dextran by Negatively Charged Ultrafiltration Membranes

1993 ◽  
Vol 22 (4) ◽  
pp. 663-666 ◽  
Author(s):  
Takaomi Kobayashi ◽  
Takuro Miyamoto ◽  
Toshihiko Nagai ◽  
Nobuyuki Fujii
Keyword(s):  
Ultrafiltration Membranes ◽  
Charged Ultrafiltration Membranes

Recovery of small dye molecules from aqueous solutions using charged ultrafiltration membranes

2015 ◽  
Vol 284 ◽  
pp. 58-64 ◽  
Author(s):  
Xiuwen Chen ◽  
Yiru Zhao ◽  
Jennifer Moutinho ◽  
Jiahui Shao ◽  
Andrew L. Zydney ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Dye Molecules ◽  
Ultrafiltration Membranes ◽  
Charged Ultrafiltration Membranes
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