Comparison of microbial community composition and activity in sulfate-reducing batch systems remediating mine drainage

2008 ◽  
Vol 101 (4) ◽  
pp. 702-713 ◽  
Author(s):  
L.P. Pereyra ◽  
S.R. Hiibel ◽  
A. Pruden ◽  
K.F. Reardon
Keyword(s):  
Microbial Community ◽  
Community Composition ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Sulfate Reducing ◽  
Batch Systems

scholarly journals Fine-scale spatial patterns in microbial community composition in an acid mine drainage

2017 ◽  
Vol 93 (10) ◽  
Author(s):  
Jie-Liang Liang ◽  
Xiao-Jing Li ◽  
Hao-Yue Shu ◽  
Pandeng Wang ◽  
Jia-Liang Kuang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Community Composition ◽  
Spatial Patterns ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Fine Scale ◽  
Acid Mine

scholarly journals Effect of Sodium Bisulfite Injection on the Microbial Community Composition in a Brackish-Water-Transporting Pipeline

2011 ◽  
Vol 77 (19) ◽  
pp. 6908-6917 ◽  
Author(s):  
Hyung Soo Park ◽  
Indranil Chatterjee ◽  
Xiaoli Dong ◽  
Sheng-Hung Wang ◽  
Christoph W. Sensen ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Composition ◽  
Microbial Community Composition ◽  
Sodium Bisulfite ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Iron Corrosion ◽  
Injection Point ◽  
Content Type ◽  
Sulfate Reducing

ABSTRACTPipelines transporting brackish subsurface water, used in the production of bitumen by steam-assisted gravity drainage, are subject to frequent corrosion failures despite the addition of the oxygen scavenger sodium bisulfite (SBS). Pyrosequencing of 16S rRNA genes was used to determine the microbial community composition for planktonic samples of transported water and for sessile samples of pipe-associated solids (PAS) scraped from pipeline cutouts representing corrosion failures. These were obtained from upstream (PAS-616P) and downstream (PAS-821TP and PAS-821LP, collected under rapid-flow and stagnant conditions, respectively) of the SBS injection point. Most transported water samples had a large fraction (1.8% to 97% of pyrosequencing reads) ofPseudomonasnot found in sessile pipe samples. The sessile population of PAS-616P had methanogens (Methanobacteriaceae) as the main (56%) community component, whereasDeltaproteobacteriaof the generaDesulfomicrobiumandDesulfocapsawere not detected. In contrast, PAS-821TP and PAS-821LP had lower fractions (41% and 0.6%) ofMethanobacteriaceaearchaea but increased fractions of sulfate-reducingDesulfomicrobium(18% and 48%) and of bisulfite-disproportionatingDesulfocapsa(35% and 22%) bacteria. Hence, SBS injection strongly changed the sessile microbial community populations. X-ray diffraction analysis of pipeline scale indicated that iron carbonate was present both upstream and downstream, whereas iron sulfide and sulfur were found only downstream of the SBS injection point, suggesting a contribution of the bisulfite-disproportionating and sulfate-reducing bacteria in the scale to iron corrosion. Incubation of iron coupons with pipeline waters indicated iron corrosion coupled to the formation of methane. Hence, both methanogenic and sulfidogenic microbial communities contributed to corrosion of pipelines transporting these brackish waters.

scholarly journals Passive Treatment for Acid Mine Drainage Partially Restores Microbial Community Structure in Different Stream Habitats

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3300
Author(s):  
Alexis N. Neff ◽  
Dean M. DeNicola ◽  
Chris Maltman
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Acid Mine Drainage ◽  
Microbial Communities ◽  
Community Composition ◽  
Microbial Community Structure ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Taxonomic Composition ◽  
Passive Treatment

The assessment of the degree to which biological communities in streams impaired by acid mine drainage (AMD) are restored by passive treatment has focused primarily on eukaryotic-cell organisms and microbial processes. The responses of microbial community structure to passive treatment have received much less attention, even though functional processes such as nutrient cycling and organic matter decomposition depend on taxonomic composition. Our objective was to determine the degree to which passive treatment restored microbial communities in three types of habitats: aqueous, leaf, and sediment. To assess their recovery, we compared the community composition in these habitats based on 16S rRNA gene sequencing at three different stream sites: an untreated AMD site (U), a remediated site below AMD passive treatment (T), and an unimpaired reference site (R). The acidity, conductivity, and soluble metal concentrations at T were found to be elevated compared to R, but generally 1–2 orders of magnitude less than at U. Microbial community composition was found to be synergistically affected by habitat type and AMD impact, with the similarity among communities in the three habitats increasing with the severity of the AMD. Sediment- and leaf-associated microbial communities at U were characterized by taxa that are tolerant to severe AMD. The absence of the nitrogen oxidizing bacterium Nitrospira in sediment communities at T and U was found to correspond to higher NH4+ concentrations compared to R, possibly because of the presence of iron oxyhydroxide precipitate. In contrast, the microbial composition was found to be similar between the T and R sites for both aqueous and leaf communities, indicating that passive treatment was more able to restore these communities to the reference condition than sediment communities. The remediation of AMD streams should consider the habitat-specific responses of microbial community composition and be guided by future studies that empirically couple changes in taxonomic composition to measured functional processes.

scholarly journals Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization

1998 ◽  
Vol 64 (7) ◽  
pp. 2691-2696 ◽  
Author(s):  
Enric Llobet-Brossa ◽  
Ramon Rosselló-Mora ◽  
Rudolf Amann
Keyword(s):  
In Situ Hybridization ◽  
Microbial Community ◽  
Community Composition ◽  
Wadden Sea ◽  
Large Fraction ◽  
Microbial Community Composition ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Sea Coast

ABSTRACT The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4′,6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of theCytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria.

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