A fast cell loading and high-throughput microfluidic system for long-term cell culture in zero-flow environments

2008 ◽  
Vol 101 (1) ◽  
pp. 190-195 ◽  
Author(s):  
Chunxiong Luo ◽  
Xuejun Zhu ◽  
Tao Yu ◽  
Xianjia Luo ◽  
Qi Ouyang ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Microfluidic System ◽  
Cell Loading ◽  
Zero Flow

High-throughput microfluidic system for long-term bacterial colony monitoring and antibiotic testing in zero-flow environments

2011 ◽  
Vol 26 (5) ◽  
pp. 1993-1999 ◽  
Author(s):  
Peng Sun ◽  
Yang Liu ◽  
Jun Sha ◽  
Zhiyun Zhang ◽  
Qin Tu ◽  
...  
Keyword(s):  
High Throughput ◽  
Microfluidic System ◽  
Bacterial Colony ◽  
Zero Flow

High-throughput microfluidic system for monitoring diffusion-based monolayer yeast cell culture over long time periods

2009 ◽  
Vol 11 (5) ◽  
pp. 981-986 ◽  
Author(s):  
Chunxiong Luo ◽  
Lingli Jiang ◽  
Shibo Liang ◽  
Qi Ouyang ◽  
Hang Ji ◽  
...  
Keyword(s):  
Cell Culture ◽  
Yeast Cell ◽  
High Throughput ◽  
Microfluidic System ◽  
Long Time ◽  
Time Periods

Versatile on-stage microfluidic system for long term cell culture, micromanipulation and time lapse assays

2018 ◽  
Vol 101 ◽  
pp. 66-74 ◽  
Author(s):  
Yao-Xiong Huang ◽  
Chun-Lan He ◽  
Ping Wang ◽  
Yan-Ting Pan ◽  
Wei-Wei Tuo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Time Lapse ◽  
Microfluidic System

scholarly journals Engineered Microvessel for Cell Culture in Simulated Microgravity

2021 ◽  
Vol 22 (12) ◽  
pp. 6331
Author(s):  
Mei ElGindi ◽  
Ibrahim Hamed Ibrahim ◽  
Jiranuwat Sapudom ◽  
Anna Garcia-Sabate ◽  
Jeremy C. M. Teo
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Simulated Microgravity ◽  
Culture Media ◽  
Large Cell ◽  
Cell Types ◽  
Suitable Material ◽  
Space Flights ◽  
Hypoxic Conditions

As the number of manned space flights increase, studies on the effects of microgravity on the human body are becoming more important. Due to the high expense and complexity of sending samples into space, simulated microgravity platforms have become a popular way to study these effects on earth. In addition, simulated microgravity has recently drawn the attention of regenerative medicine by increasing cell differentiation capability. These platforms come with many advantages as well as limitations. A main limitation for usage of these platforms is the lack of high-throughput capability due to the use of large cell culture vessels. Therefore, there is a requirement for microvessels for microgravity platforms that limit waste and increase throughput. In this work, a microvessel for commercial cell culture plates was designed. Four 3D printable (polycarbonate (PC), polylactic acid (PLA) and resin) and castable (polydimethylsiloxane (PDMS)) materials were assessed for biocompatibility with adherent and suspension cell types. PDMS was found to be the most suitable material for microvessel fabrication, long-term cell viability and proliferation. It also allows for efficient gas exchange, has no effect on cell culture media pH and does not induce hypoxic conditions. Overall, the designed microvessel can be used on simulated microgravity platforms as a method for long-term high-throughput biomedical studies.

scholarly journals A zero-flow microfluidics for long-term cell culture and detection

AIP Advances ◽  
2015 ◽  
Vol 5 (4) ◽  
pp. 041310 ◽  
Author(s):  
Shengbo Sang ◽  
Xiaoliang Tang ◽  
Qiliang Feng ◽  
Aoqun Jian ◽  
Wendong Zhang
Keyword(s):  
Cell Culture ◽  
Zero Flow

Islet-on-a-chip: Biomimetic micropillar-based microfluidic system for three-dimensional pancreatic islet cell culture

2021 ◽  
Vol 183 ◽  
pp. 113215
Author(s):  
Patrycja Sokolowska ◽  
Kamil Zukowski ◽  
Justyna Janikiewicz ◽  
Elzbieta Jastrzebska ◽  
Agnieszka Dobrzyn ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Three Dimensional ◽  
Microfluidic System ◽  
Pancreatic Islet Cell ◽  
Islet Cell Culture

scholarly journals Organ-specific, multimodal, wireless optoelectronics for high-throughput phenotyping of peripheral neural pathways

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Woo Seok Kim ◽  
Sungcheol Hong ◽  
Milenka Gamero ◽  
Vivekanand Jeevakumar ◽  
Clay M. Smithhart ◽  
...  
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Optoelectronic Device ◽  
Antenna System ◽  
Neural Pathways ◽  
Organ Specific ◽  
Coil Antenna ◽  
Sensory Fibers

AbstractThe vagus nerve supports diverse autonomic functions and behaviors important for health and survival. To understand how specific components of the vagus contribute to behaviors and long-term physiological effects, it is critical to modulate their activity with anatomical specificity in awake, freely behaving conditions using reliable methods. Here, we introduce an organ-specific scalable, multimodal, wireless optoelectronic device for precise and chronic optogenetic manipulations in vivo. When combined with an advanced, coil-antenna system and a multiplexing strategy for powering 8 individual homecages using a single RF transmitter, the proposed wireless telemetry enables low cost, high-throughput, and precise functional mapping of peripheral neural circuits, including long-term behavioral and physiological measurements. Deployment of these technologies reveals an unexpected role for stomach, non-stretch vagal sensory fibers in suppressing appetite and demonstrates the durability of the miniature wireless device inside harsh gastric conditions.

scholarly journals Real-time imaging of cellular forces using optical interference

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Andrew T. Meek ◽  
Nils M. Kronenberg ◽  
Andrew Morton ◽  
Philipp Liehm ◽  
Jan Murawski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
High Throughput ◽  
Mechanical Activity ◽  
Dynamic Processes ◽  
Imaging Method ◽  
Neonatal Cardiomyocytes ◽  
A Cell ◽  
Cellular Forces ◽  
Time Acquisition

AbstractImportant dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution.

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