Scale-up from shake flasks to fermenters in batch and continuous mode withCorynebacterium glutamicum on lactic acid based on oxygen transfer and pH

2007 ◽  
Vol 98 (4) ◽  
pp. 800-811 ◽  
Author(s):  
Juri M. Seletzky ◽  
Ute Noak ◽  
Jens Fricke ◽  
Eike Welk ◽  
Werner Eberhard ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Oxygen Transfer ◽  
Scale Up ◽  
Continuous Mode ◽  
Shake Flasks

scholarly journals Development of Four Unit Processes for Biobased PLA Manufacturing

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Chae Hwan Hong ◽  
Si Hwan Kim ◽  
Ji-Yeon Seo ◽  
Do Suck Han
Keyword(s):  
Lactic Acid ◽  
Ring Opening ◽  
Lactic Acid Production ◽  
Scale Up ◽  
Catalyst System ◽  
Ring Opening Polymerization ◽  
Acid Fermentation ◽  
Manufacturing Method ◽  
Renewable Biomass ◽  
Microbial Productivity

Polylactide (PLA), which is one of the most important biocompatible polyesters that are derived from annually renewable biomass such as corn and sugar beets, has attracted much attention for automotive parts application. The manufacturing method of PLA is the ring-opening polymerization of the dimeric cyclic ester of lactic acid, lactide. For the stereocomplex PLA, we developed the four unit processes, fermentation, separation, lactide conversion, and polymerization. Fermentation of sugars to D-lactic acid is little studied, and its microbial productivity is not well known. Therefore, we investigated D-lactic acid fermentation with a view to obtaining the strains capable of producing D-lactic acid, and we got a maximum lactic acid production 60 g/L. Lactide is prepared by a two-step process: first, the lactic acid is converted into oligo(lactic acid) by a polycondensation reaction; second, the oligo(lactic acid) is thermally depolymerized to form the cyclic lactide via an unzipping mechanism. Through catalyst screening test for polycondensation and depolymerization reactions, we got a new method which shortens the whole reaction time 50% the level of the conventional method. Poly(L-lactide) was obtained from the ring-opening polymerization of L-lactide. We investigated various catalysts and polymerization conditions. Finally, we got the best catalyst system and the scale-up technology.

scholarly journals A Process Study of Lactic Acid Production from Phragmites australis Straw by a Thermophilic Bacillus coagulans Strain under Non-Sterilized Conditions

Processes ◽  
2018 ◽  
Vol 6 (10) ◽  
pp. 175 ◽  
Author(s):  
Yuming Zhang ◽  
Mengran Li ◽  
Tian Nie ◽  
Zhihua Ni
Keyword(s):  
Lactic Acid ◽  
Phragmites Australis ◽  
Lactic Acid Production ◽  
Scale Up ◽  
Bacillus Coagulans ◽  
Dilute Acid Pretreatment ◽  
Integrated Process ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Process Study

Phragmites australis straw (PAS) is an abundant and renewable wetland lignocellulose. Bacillus coagulans IPE22 is a robust thermophilic strain with pentose-utilizing capability and excellent resistance to growth inhibitors. This work is focused on the process study of lactic acid (LA) production from P. australis lignocellulose which has not been attempted previously. By virtue of thermophilic feature of strain IPE22, two fermentation processes (i.e., separated process and integrated process), were developed and compared under non-sterilized conditions. The integrated process combined dilute-acid pretreatment, hemicellulosic hydrolysates fermentation, and cellulose utilization. Sugars derived from hemicellulosic hydrolysates and cellulose enzymatic hydrolysis were efficiently fermented to LA in a single vessel. Using the integrated process, 41.06 g LA was produced from 100 g dry PAS. The established integrated process results in great savings in terms of time and labor, and the fermentation process under non-sterilized conditions is easy to scale up for economical production of lactic acid from PAS.

scholarly journals Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

2003 ◽  
Vol 46 (3) ◽  
pp. 455-459 ◽  
Author(s):  
Georgina L. Michelena ◽  
Aidín Martínez ◽  
Antonio Bell ◽  
Emilia Carrera ◽  
Roxana Valencia
Keyword(s):  
Oxygen Transfer ◽  
Enzyme Production ◽  
Batch Fermentation ◽  
Leuconostoc Mesenteroides ◽  
Scale Up ◽  
Oxygen Transfer Rate ◽  
Maximum Growth ◽  
Aeration Rate ◽  
Fed Batch ◽  
Fed Batch Fermentation

Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.

Bioreactor studies on the endophytic fungus Entrophospora infrequens for the production of an anticancer alkaloid camptothecin

2006 ◽  
Vol 52 (3) ◽  
pp. 189-196 ◽  
Author(s):  
Touseef Amna ◽  
Satish C Puri ◽  
V Verma ◽  
Jai P Sharma ◽  
Rajinder K Khajuria ◽  
...  
Keyword(s):  
Cell Mass ◽  
Scale Up ◽  
Maximum Yield ◽  
Dry Mass ◽  
Spectroscopic Studies ◽  
Physicochemical Analysis ◽  
Culture Broth ◽  
Nothapodytes Foetida ◽  
Bark Tissue ◽  
Shake Flasks

Twigs (young and old) from Nothapodytes foetida growing in the Jammu and Mahabaleshwar regions in India were used for the isolation of 52 strains of endophytic fungi and were tested for their ability to produce the anticancer alkaloid camptothecin. One of the isolates from the inner bark tissue of the N. foetida plant growing in the Jammu region of J&K state, India, was found to produce detectable quantities of camptothecin and its derivatives when grown in a semi-synthetic liquid medium. Camptothecin was identified by physicochemical analysis and further confirmed by spectroscopic studies. No camptothecin was detected in zero time cultures or in uninoculated culture broth. The maximum yield of camptothecin was 0.575 ± 0.031 mg/100 g of dry cell mass in 96 h in shake flasks, whereas 4.96 ± 0.73 mg/100 g of dry mass was recorded in 48 h in a bioreactor.Key words: endophytes, camptothecin, anticancer drug, Nothapodytes foetida, Entrophospora infrequens, bioreactor, scale-up.

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