The YfiD protein contributes to the pyruvate formate-lyase flux in anEscherichia coli arcA mutant strain

In the present study, an attempt was made to study the effect of plant extract on Sexual behaviour of Mutant Strain (Curled) of Drosophila melanogaster. The LC50 has been estimated with 1% of the food media. The virgin females and males were isolated and fed with normal food media for three days. Then sub-lethal concentrations of 0.625 μl / 100 ml food, 1.2 μl /100 ml food, 2.5μl /100 / food of nicotine were mixed in food medium and allowed in flies to feed for two days. Then appropriate combination of untreated / treated males and females were introduced into the mating chamber. Courtship latency, mating latency and copulation duration were studied. After observation of the behaviour, mated flies were allowed to produce progeny. The sexual behaviour of bachelor male and virgin female obtained in the progeny was also studied. The pooled data were analyzed by student t-test and the result indicates p-value significant at 0.05 levels. The courtship latency was affected by in treatment but it is neither dose dependent nor sex dependent.

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Molecular Basis of UV-Sensitive Mutant Strain MBH3 of Haemophilus Influenzae Rd: Identification of Mutation in the uvrA Gene

Journal of Environmental Pathology Toxicology and Oncology ◽

10.1615/jenvironpatholtoxicoloncol.v20.i1.50 ◽

2001 ◽

Vol 20 (1) ◽

pp. 6 ◽

Cited By ~ 1

Author(s):

Rashna D. Balsara ◽

Vasudha P. Joshi

Keyword(s):

Haemophilus Influenzae ◽

Mutant Strain ◽

Molecular Basis ◽

Sensitive Mutant

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Isolation of a mutant strain of Pseudomonas sp. ATCC 31461 exhibiting elevated polysaccharide production

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj/jim/7000278 ◽

2002 ◽

Vol 29 (4) ◽

pp. 185-188

Author(s):

T P West

Keyword(s):

Mutant Strain ◽

Pseudomonas Sp ◽

Polysaccharide Production

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The role of Zur-regulated lipoprotein A in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii

BMC Microbiology ◽

10.1186/s12866-020-02083-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Nayeong Kim ◽

Hyo Jeong Kim ◽

Man Hwan Oh ◽

Se Yeon Kim ◽

Mi Hyun Kim ◽

...

Keyword(s):

Acinetobacter Baumannii ◽

Outer Membrane ◽

Mutant Strain ◽

Antimicrobial Susceptibility ◽

Type Strain ◽

Wild Type Strain ◽

Membrane Vesicles ◽

Outer Membrane Vesicles ◽

Wild Type ◽

Bacterial Morphology

Abstract Background Zinc uptake-regulator (Zur)-regulated lipoprotein A (ZrlA) plays a role in bacterial fitness and overcoming antimicrobial exposure in Acinetobacter baumannii. This study further characterized the zrlA gene and its encoded protein and investigated the roles of the zrlA gene in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles (OMVs) in A. baumannii ATCC 17978. Results In silico and polymerase chain reaction analyses showed that the zrlA gene was conserved among A. baumannii strains with 97–100% sequence homology. Recombinant ZrlA protein exhibited a specific enzymatic activity of D-alanine-D-alanine carboxypeptidase. Wild-type A. baumannii exhibited more morphological heterogeneity than a ΔzrlA mutant strain during stationary phase. The ΔzrlA mutant strain was more susceptible to gentamicin than the wild-type strain. Sizes and protein profiles of OMVs were similar between the wild-type and ΔzrlA mutant strains, but the ΔzrlA mutant strain produced 9.7 times more OMV particles than the wild-type strain. OMVs from the ΔzrlA mutant were more cytotoxic in cultured epithelial cells than OMVs from the wild-type strain. Conclusions The present study demonstrated that A. baumannii ZrlA contributes to bacterial morphogenesis and antimicrobial resistance, but its deletion increases OMV production and OMV-mediated host cell cytotoxicity.

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Ultrastructure and pyruvate formate-lyase radical quenching property of the multienzymic AdhE protein of Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)37154-6 ◽

1992 ◽

Vol 267 (25) ◽

pp. 18073-18079 ◽

Cited By ~ 1

Author(s):

D Kessler ◽

W Herth ◽

J Knappe

Keyword(s):

Escherichia Coli ◽

Pyruvate Formate Lyase

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Improvement of the attenuated mutant strain of Brucella melitensis Rev1 as a potential vaccine candidate

Biologia ◽

10.1007/s11756-021-00695-z ◽

2021 ◽

Author(s):

Elham Mehdizadeh Marzenaki ◽

Ali Reza Saeedinia ◽

Mehdi Zeinoddini ◽

Ali Asghar Deldar

Keyword(s):

Mutant Strain ◽

Vaccine Candidate ◽

Brucella Melitensis ◽

Potential Vaccine Candidate ◽

Potential Vaccine

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Linkage group XIX of Chlamydomonas reinhardtii has a linear map.

Genetics ◽

10.1093/genetics/133.4.865 ◽

1993 ◽

Vol 133 (4) ◽

pp. 865-874

Author(s):

J A Holmes ◽

D E Johnson ◽

S K Dutcher

Keyword(s):

Linkage Group ◽

Chlamydomonas Reinhardtii ◽

Mutant Strain ◽

Meiotic Recombination ◽

Temperature Sensitive ◽

Unusual Property ◽

Linkage Groups ◽

Excess Number ◽

Chiasma Interference ◽

Double Crossovers

Abstract Linkage group XIX (or the UNI linkage group) of Chlamydomonas reinhardtii has been reported to show a circular meiotic recombination map. A circular map predicts the existence of strong chiasma and chromatid interference, which would lead to an excess number of two-strand double crossovers during meiosis. We have tested this prediction in multipoint crosses. Our results are consistent with a linear linkage group that shows positive chiasma interference and no chromatid interference. Chiasma interference occurs both within arms and across the centromere. Of the original loci that contributed to the circular map, we find that two map to other linkage groups and a third cannot be retested because the mutant strain that defined it has been lost. A second reported unusual property for linkage group XIX was the increase in meiotic recombination with increases in temperature during a period that precedes the onset of meiosis. Although we observed changes in recombination frequencies in some intervals on linkage group XIX in crosses to CC-1952, and in strains heterozygous for the mutation ger1 at 16 degrees, we also show that our strains do not exhibit the previously observed patterns of temperature-sensitive recombination for two different pairs of loci on linkage group XIX. We conclude that linkage group XIX has a linear genetic map that is not significantly different from other Chlamydomonas linkage groups.

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The tamas Gene, Identified as a Mutation That Disrupts Larval Behavior in Drosophila melanogaster, Codes for the Mitochondrial DNA Polymerase Catalytic Subunit (DNApol-γ125)

Genetics ◽

10.1093/genetics/153.4.1809 ◽

1999 ◽

Vol 153 (4) ◽

pp. 1809-1824 ◽

Cited By ~ 8

Author(s):

Balaji Iyengar ◽

John Roote ◽

Ana Regina Campos

Keyword(s):

Mitochondrial Dna ◽

Drosophila Melanogaster ◽

Mutant Strain ◽

Dna Polymerase ◽

Complementation Group ◽

Behavioral Changes ◽

Catalytic Subunit ◽

Primary Deficit ◽

Mitochondrial Dna Polymerase ◽

Food Substrate

AbstractFrom a screen of pupal lethal lines of Drosophila melanogaster we identified a mutant strain that displayed a reproducible reduction in the larval response to light. Moreover, this mutant strain showed defects in the development of the adult visual system and failure to undergo behavioral changes characteristic of the wandering stage. The foraging third instar larvae remained in the food substrate for a prolonged period and died at or just before pupariation. Using a new assay for individual larval photobehavior we determined that the lack of response to light in these mutants was due to a primary deficit in locomotion. The mutation responsible for these phenotypes was mapped to the lethal complementation group l(2)34Dc, which we renamed tamas (translated from Sanskrit as “dark inertia”). Sequencing of mutant alleles demonstrated that tamas codes for the mitochondrial DNA polymerase catalytic subunit (DNApol-γ125).

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