Molecular analysis of successful cell line selection in transfected GS-NS0 myeloma cells

2006 ◽  
Vol 96 (2) ◽  
pp. 337-348 ◽  
Author(s):  
Louise M. Barnes ◽  
Catherine M. Bentley ◽  
Nicola Moy ◽  
Alan J. Dickson
Keyword(s):  
Cell Line ◽  
Molecular Analysis ◽  
Myeloma Cells ◽  
Line Selection ◽  
Cell Line Selection

scholarly journals A Place for High-Throughput Electrophysiology in Cardiac Safety: Screening hERG Cell Lines and Novel Compounds with the Ion Works HTTM System

2005 ◽  
Vol 10 (8) ◽  
pp. 832-840 ◽  
Author(s):  
Heather Guthrie ◽  
Frederick S. Livingston ◽  
Ueli Gubler ◽  
Ralph Garippa
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Torsades De Pointes ◽  
Delayed Rectifier ◽  
Drug Candidates ◽  
Delayed Rectifier Potassium Channel ◽  
A Cell ◽  
Line Selection ◽  
Herg Channels ◽  
Cell Line Selection

Several commercially available pharmaceutical compounds have been shown to block the I Krcurrent of the cardiac action potential. This effect can cause a prolongation of the electrocardiogram QTinterval and a delay in ventricular repolarization. The Food and Drug Administration recommends that all new potential drug candidates be assessed for I Krblock to avoid a potentially lethal cardiac arrhythmia known as torsades de pointes. Direct compound interaction with the human ether-a-go-go– related gene (hERG) product, a delayed rectifier potassium channel, has been identified as a molecular mechanism of I Kr block. One strategy to identify compounds withh ERGliability is to monitor hERGcurrent inhibition using electrophysiology techniques. The authors describe the Ion Works HT ™instrument as a tool for screening cell lines expressing hERG channels. Based on current amplitude and stability criteria, a cell line was selected and used to perform a 300-compound screen. The screen was able to identify compounds with hERG activity within projects that spanned different therapeutic areas. The cell line selection and optimization, as well as the screening abilities of the Ion Works HT ™system, provide a powerful means of assessinghERGactive compounds early in the drug discovery pipeline.

Rescue of defective SV40 from a transformed mouse 3T3 cell line: Selection of a specific defective

Virology ◽  
1974 ◽  
Vol 60 (2) ◽  
pp. 342-352 ◽  
Author(s):  
Kunito Yoshiike ◽  
Akemi Furuno ◽  
Seijiro Uchida
Keyword(s):  
Cell Line ◽  
Line Selection ◽  
Cell Line Selection ◽  
Selection Of

scholarly journals Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Rileen Sinha ◽  
Andrew G. Winer ◽  
Michael Chevinsky ◽  
Christopher Jakubowski ◽  
Ying-Bei Chen ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Renal Cancer ◽  
Cancer Cell Lines ◽  
Renal Cancer Cell ◽  
Line Selection ◽  
Cell Line Selection

scholarly journals Does earlier use of productivity enhancers during cell line selection lead to the identification of more productive cell lines?

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Alison J Porter ◽  
Atul Mohindra ◽  
Juana Maria Porter ◽  
Andrew J Racher
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Line Selection ◽  
Cell Line Selection

scholarly journals Cell line selection using the Duetz Microflask system

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Steve R C Warr ◽  
Sharon L White ◽  
Yuen-Ting Chim ◽  
Jai Patel ◽  
Hella Bosteels
Keyword(s):  
Cell Line ◽  
Line Selection ◽  
Cell Line Selection
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