High-throughput and real-time study of single cell electroporation using microfluidics: Effects of medium osmolarity

2006 ◽  
Vol 95 (6) ◽  
pp. 1116-1125 ◽  
Author(s):  
Hsiang-Yu Wang ◽  
Chang Lu
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Time Study ◽  
Cell Electroporation ◽  
Medium Osmolarity ◽  
Single Cell Electroporation

scholarly journals Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 856
Author(s):  
Yifei Ye ◽  
Xiaofeng Luan ◽  
Lingqian Zhang ◽  
Wenjie Zhao ◽  
Jie Cheng ◽  
...  
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Intracellular Delivery ◽  
Microfluidic System ◽  
Label Free ◽  
Precise Control ◽  
Cell Electroporation ◽  
Single Cell Electroporation

The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporation and simultaneous impedance monitoring in a constriction microchannel. When single cells (A549) were continuously passing through the constriction microchannel, a localized high electric field was applied on the cell membrane, which resulted in highly efficient (up to 96.6%) electroporation. During a single cell entering the constriction channel, an abrupt impedance drop was noticed and demonstrated to be correlated with the occurrence of electroporation. Besides, while the cell was moving in the constriction channel, the stabilized impedance showed the capability to quantify the electroporation extent. The correspondence of the impedance variation and electroporation was validated by the intracellular delivery of the fluorescence indicator (propidium iodide). Based on the obtained results, this system is capable of precise control of electroporation and real-time, label-free impedance assessment, providing a potential tool for intracellular delivery and other biomedical applications.

Single-cell electroporation arrays with real-time monitoring and feedback control

Lab on a Chip ◽  
2007 ◽  
Vol 7 (4) ◽  
pp. 457 ◽  
Author(s):  
Michelle Khine ◽  
Cristian Ionescu-Zanetti ◽  
Andrew Blatz ◽  
Lee-Ping Wang ◽  
Luke P. Lee
Keyword(s):  
Feedback Control ◽  
Single Cell ◽  
Real Time ◽  
Real Time Monitoring ◽  
Cell Electroporation ◽  
Single Cell Electroporation

Localized, Macromolecular Transport for Thin, Adherent, Single Cells Via an Automated, Single Cell Electroporation Biomanipulator

2013 ◽  
Vol 60 (11) ◽  
pp. 3113-3123 ◽  
Author(s):  
Kelly Sakaki ◽  
Hadi Esmaeilsabzali ◽  
Shabnam Massah ◽  
Gratien G. Prefontaine ◽  
Nikolai Dechev ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Macromolecular Transport ◽  
Cell Electroporation ◽  
Single Cell Electroporation

High-Throughput and Real-Time Monitoring of Single-Cell Extracellular pH Based on Polyaniline Microarrays

2021 ◽  
Author(s):  
Lihui Yang ◽  
Xiaobo Liu ◽  
Bing Yin ◽  
Xunxun Deng ◽  
Xiaotong Lin ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Extracellular Ph ◽  
Real Time Monitoring
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