scholarly journals Tyrosine-based “Activatable Pro-Tag”: Enzyme-catalyzed protein capture and release

2006 ◽  
Vol 93 (6) ◽  
pp. 1207-1215 ◽  
Author(s):  
Angela T. Lewandowski ◽  
David A. Small ◽  
Tianhong Chen ◽  
Gregory F. Payne ◽  
William E. Bentley
Keyword(s):  
Protein Capture ◽  
Capture And Release

scholarly journals Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 267
Author(s):  
Chen Jiao ◽  
Franziska Obst ◽  
Martin Geisler ◽  
Yunjiao Che ◽  
Andreas Richter ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Disulfide Bonds ◽  
Stimuli Responsive ◽  
Disulfide Exchange ◽  
Protein Capture ◽  
Wide Range ◽  
Redox Responsive ◽  
Potential Applications ◽  
Capture And Release ◽  
Cross Linker

Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.

scholarly journals Generic high-capacity protein capture and release by pH control

2020 ◽  
Vol 56 (44) ◽  
pp. 5889-5892 ◽  
Author(s):  
G. Ferrand-Drake del Castillo ◽  
R. L. N. Hailes ◽  
Z. Adali-Kaya ◽  
T. Robson ◽  
Andreas Dahlin
Keyword(s):  
High Capacity ◽  
Ph Control ◽  
Polyelectrolyte Brushes ◽  
Protein Capture ◽  
Capture And Release

A new and generic method for protein capture-release by polyelectrolyte brushes and pH control.

Unusual Nanofractal Microparticles for Rapid Protein Capture and Release

Small ◽  
2021 ◽  
pp. 2102802
Author(s):  
Yongyang Song ◽  
Xuefang Dong ◽  
Danyi Shang ◽  
Xiaofei Zhang ◽  
Xiuling Li ◽  
...  
Keyword(s):  
Protein Capture ◽  
Capture And Release

Biomimetic recognition strategy for efficient capture and release of circulating tumor cells

2021 ◽  
Vol 188 (6) ◽  
Author(s):  
Ji Zheng ◽  
Dayong Li ◽  
Jin Jiao ◽  
Chengjie Duan ◽  
Youjing Gong ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Capture And Release

Clog-free cell filtration using resettable cell traps

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2657-2665 ◽  
Author(s):  
William Beattie ◽  
Xi Qin ◽  
Lin Wang ◽  
Hongshen Ma
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Free Cell ◽  
Separation Mechanism ◽  
Cell Filtration ◽  
Capture And Release

A microfluidic cell separation mechanism created using constrictions with adjustable size that can selectively capture and release cells, thereby enabling high throughput size and deformability based cell separation without clogging.

Azobenzene-Functionalized Metal-Organic Polyhedra for the Optically Responsive Capture and Release of Guest Molecules

2014 ◽  
Vol 53 (23) ◽  
pp. 5842-5846 ◽  
Author(s):  
Jinhee Park ◽  
Lin-Bing Sun ◽  
Ying-Pin Chen ◽  
Zachary Perry ◽  
Hong-Cai Zhou
Keyword(s):  
Guest Molecules ◽  
Metal Organic ◽  
Capture And Release

DNA nano-carrier for repeatable capture and release of biomolecules

Nanoscale ◽  
2015 ◽  
Vol 7 (41) ◽  
pp. 17397-17403 ◽  
Author(s):  
Michael T. Hwang ◽  
Preston B. Landon ◽  
Joon Lee ◽  
Alexander Mo ◽  
Brian Meckes ◽  
...  
Keyword(s):  
Capture And Release
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