Three-dimensional cell seeding and growth in radial-flow perfusion bioreactor for in vitro tissue reconstruction

2006 ◽  
Vol 93 (5) ◽  
pp. 947-954 ◽  
Author(s):  
Tatsuya Kitagawa ◽  
Tetsuji Yamaoka ◽  
Reiko Iwase ◽  
Akira Murakami
Keyword(s):  
Radial Flow ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Cell Seeding ◽  
Flow Perfusion ◽  
Tissue Reconstruction ◽  
Flow Perfusion Bioreactor ◽  
Dimensional Cell

scholarly journals A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Alexander M. Sailon ◽  
Alexander C. Allori ◽  
Edward H. Davidson ◽  
Derek D. Reformat ◽  
Robert J. Allen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Density ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Peripheral Cell ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds.Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core.Results. By day 8, static scaffolds had a periphery cell density of , while in the core it was . Flow-perfused scaffolds demonstrated peripheral cell density of and core density of at day 8.Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems.

scholarly journals A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures

2018 ◽  
Vol 24 (10) ◽  
pp. 585-595 ◽  
Author(s):  
Jakob Schmid ◽  
Sascha Schwarz ◽  
Robert Meier-Staude ◽  
Stefanie Sudhop ◽  
Hauke Clausen-Schaumann ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Cell Seeding ◽  
Bioreactor System ◽  
Dimensional Cell

scholarly journals Design and Functional Testing of a Multichamber Perfusion Platform for Three-Dimensional Scaffolds

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Marco Piola ◽  
Monica Soncini ◽  
Marco Cantini ◽  
Nasser Sadr ◽  
Giulio Ferrario ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Perfusion Culture ◽  
Homogeneous Distribution ◽  
Porous Scaffolds ◽  
Cell Seeding ◽  
Flow Perfusion ◽  
Culture Viability ◽  
Compact Size ◽  
Dynamic Cell

Perfusion culture systems are widely used in tissue engineering applications for enhancing cell culture viability in the core of three-dimensional scaffolds. In this work, we present a multichamber confined-flow perfusion system, designed to provide a straightforward platform for three-dimensional dynamic cell cultures. The device comprises 6 culture chambers allowing independent and simultaneous experiments in controlled conditions. Each chamber consists of three parts: a housing, a deformable scaffold-holder cartridge, and a 7 mL reservoir, which couples water-tightly with the housing compressing the cartridge. Short-term dynamic cell seeding experiments were carried out with MC3T3-E1 cells seeded into polycaprolactone porous scaffolds. Preliminary results revealed that the application of flow perfusion through the scaffold favored the penetration of the cells to its interior, producing a more homogeneous distribution of cells with respect to dropwise or injection seeding methods. The culture chamber layout was conceived with the aim of simplifying the user operations under laminar flow hood and minimizing the risks for contamination during handling and operation. Furthermore, a compact size, a small number of components, and the use of bayonet couplings ensured a simple, fast, and sterility-promoting assembling. Finally, preliminaryin vitrotests proved the efficacy of the system in enhancing cell seeding efficiency, opening the way for further studies addressing long-term scaffold colonization.

Novel dual-flow perfusion bioreactor for in vitro pre-screening of nanoparticles delivery: design, characterization and testing

2021 ◽  
Author(s):  
Maria Elena Lombardo ◽  
Francesco Carfì Pavia ◽  
Emanuela Fabiola Craparo ◽  
Elisa Capuana ◽  
Gennara Cavallaro ◽  
...  
Keyword(s):  
Perfusion Bioreactor ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

Advanced in vitro management of three-dimensional cell cultures and explanted tissue

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S145
Author(s):  
S. Kress ◽  
D. Egger ◽  
C. Kasper
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Dimensional Cell

A three-dimensional cell biology model of human hepatocellular carcinoma in vitro

Tumor Biology ◽  
2010 ◽  
Vol 32 (3) ◽  
pp. 469-479 ◽  
Author(s):  
Jianhua Tang ◽  
Jiefeng Cui ◽  
Rongxin Chen ◽  
Kun Guo ◽  
Xiaonan Kang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Biology ◽  
Three Dimensional ◽  
Human Hepatocellular Carcinoma ◽  
Dimensional Cell
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