Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture

2006 ◽  
Vol 93 (5) ◽  
pp. 887-895 ◽  
Author(s):  
Qi Wen-tao ◽  
Zhang Ying ◽  
Ma Juan ◽  
Guo Xin ◽  
Xie Yu-bing ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Animal Cell ◽  
Gompertz Model ◽  
Animal Cell Culture ◽  
Seeding Density ◽  
Cell Seeding ◽  
Cell Seeding Density ◽  
Modified Gompertz Model

Flow Cytometry in Animal Cell Culture

1993 ◽  
Vol 11 (5) ◽  
pp. 572-579 ◽  
Author(s):  
Mohamed Al-Rubeai ◽  
A. Nicholas Emery
Keyword(s):  
Cell Culture ◽  
Flow Cytometry ◽  
Animal Cell ◽  
Animal Cell Culture

scholarly journals Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent manner

2018 ◽  
Vol 33 (4) ◽  
pp. 576-589 ◽  
Author(s):  
Devika M Varma ◽  
Michelle S DiNicolas ◽  
Steven B Nicoll
Keyword(s):  
Extracellular Matrix ◽  
Minimally Invasive ◽  
Nucleus Pulposus ◽  
Seeding Density ◽  
Human Mscs ◽  
Dependent Manner ◽  
Cell Seeding ◽  
Swelling Properties ◽  
Matrix Deposition ◽  
Cell Seeding Density

Low back pain is a major cause for disability and is closely linked to intervertebral disc degeneration. Mechanical and biological dysfunction of the nucleus pulposus in the disc has been found to initiate intradiscal degenerative processes. Replacing or enriching the diseased nucleus pulposus with an injectable, stem cell-laden biomaterial that mimics its material properties can provide a minimally invasive strategy for biological and structural repair of the tissue. In this study, injectable, in situ-gelling carboxymethylcellulose hydrogels were developed for nucleus pulposus tissue engineering using encapsulated human marrow-derived mesenchymal stromal cells (hMSCs). With the goal of obtaining robust extracellular matrix deposition and faster construct maturation, two cell-seeding densities, 20 × 106 cells/ml and 40 × 106 cells/ml, were examined. The constructs were fabricated using a redox initiation system to yield covalently crosslinked, cell-seeded hydrogels via radical polymerization. Chondrogenic culture of the hydrogels over 35 days exhibited high cell viability along with deposition of proteoglycan and collagen-rich extracellular matrix, and mechanical and swelling properties similar to native human nucleus pulposus. Further, the matrix production and distribution in the carboxymethylcellulose hydrogels was found to be strongly influenced by hMSC-seeding density, with the lower cell-seeding density yielding a more favorable nucleus pulposus-specific matrix phenotype, while the rate of construct maturation was less dependent on the cell-seeding density. These findings are the first to demonstrate the utility of redox-polymerized carboxymethylcellulose hydrogels as hMSC carriers for potential minimally invasive treatment strategies for nucleus pulposus replacement.

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