Effect of the applied organic load rate on biodegradable polymer production by mixed microbial cultures in a sequencing batch reactor

2005 ◽  
Vol 93 (1) ◽  
pp. 76-88 ◽  
Author(s):  
Davide Dionisi ◽  
Mauro Majone ◽  
Giovanni Vallini ◽  
Simona Di Gregorio ◽  
Mario Beccari
Keyword(s):  
Biodegradable Polymer ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Organic Load ◽  
Microbial Cultures ◽  
Load Rate ◽  
Mixed Microbial Cultures ◽  
Organic Load Rate

Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate

2005 ◽  
Vol 80 (11) ◽  
pp. 1306-1318 ◽  
Author(s):  
Davide Dionisi ◽  
Mario Beccari ◽  
Simona Di Gregorio ◽  
Mauro Majone ◽  
Marco Petrangeli Papini ◽  
...  
Keyword(s):  
Microbial Community ◽  
Biodegradable Polymers ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Organic Load ◽  
Load Rate ◽  
Organic Load Rate

Characterization and Comparison of Biomass from Mesophilic and Thermophilic Fixed Bed Anaerobic Digesters

1992 ◽  
Vol 25 (7) ◽  
pp. 203-212 ◽  
Author(s):  
M. Soto ◽  
R. Méndez ◽  
J. M. Lema
Keyword(s):  
Kinetic Parameters ◽  
Fixed Bed ◽  
Organic Load ◽  
Biomass Distribution ◽  
Anaerobic Digesters ◽  
Long Period ◽  
Load Rate ◽  
Attached Biomass ◽  
Different Levels ◽  
Organic Load Rate

Two lab-scale mesophilic (MAF) and thermophilic (TAF) anaerobic filters treating effluents from a mussel cooking factory were operated at their maximum organic load rate (OLR) for a long period of time. The biomass profiles and the distribution of occluded and attached biomass were determined. Biomass distribution in the MAF was more homogeneous along the filter. Most of the biomass in the TAF was attached while both attached and occluded biomass was observed in the MAF. The hydrolytic, acidogenic, acetogenic and methanogenic activities of sludges at different levels were determined. An attempt is made to explain the behaviour of both reactors as a function of these activities and other kinetic parameters.

Start-up of an aerobic granular sequencing batch reactor for the treatment of winery wastewater

2009 ◽  
Vol 60 (4) ◽  
pp. 1049-1054 ◽  
Author(s):  
S. López–Palau ◽  
J. Dosta ◽  
J. Mata-Álvarez
Keyword(s):  
Organic Matter ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Particle Diameter ◽  
Granular Sludge ◽  
Average Density ◽  
Aerobic Granular Sludge ◽  
Organic Load ◽  
Winery Wastewater ◽  
Start Up

Aerobic granular sludge was cultivated in a sequencing batch reactor (SBR) in order to remove the organic matter present in winery wastewater. The formation of granules was performed using a synthetic substrate. The selection parameter was the settling time, as well as the alternation of feast-famine periods, the air velocity and the height/diameter ratio of the reactor. After 10 days of operation under these conditions, the first aggregates could be observed. Filamentous bacteria were still present in the reactor but they disappeared progressively. During the start-up, COD loading was increased from 2.7 to 22.5 kg COD/(m3 day) in order to obtain a feast period between 30 and 60 minutes. At this point, granules were quite round, with a particle diameter between 3.0 and 4.0 mm and an average density of 6 g L−1. After 120 days of operation, synthetic media was replaced by real winery wastewater, with a COD loading of 6 kg COD/(m3 day). The decrease of the organic load implied a reduction of the aggregate diameter and a density increase up to 13.2 g L−1. The effluent was free of organic matter and the solids concentration in the reactor reached 6 g VSS L−1.

scholarly journals Removal of nutrients and organic pollutants from domestic wastewater treatment by sponge-based moving bed biofilm reactor

2019 ◽  
Vol 25 (5) ◽  
pp. 652-658
Author(s):  
Huynh Tan Nhut ◽  
Nguyen Tri Quang Hung ◽  
Tran Cong Sac ◽  
Nguyen Huynh Khanh Bang ◽  
Tran Quang Tri ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Treatment Plan ◽  
Domestic Wastewater ◽  
Biofilm Reactor ◽  
Organic Load ◽  
Moving Bed Biofilm Reactor ◽  
Moving Bed ◽  
Domestic Wastewater Treatment ◽  
Load Rate ◽  
Organic Load Rate

This study evaluates the efficiency of domestic wastewater treatment via Sponge-Based Moving Bed Biofilm Reactor (S-MBBR). The laboratory-based treatment plan uses polyurethane sponge with a specific surface area was 260 m<sup>2</sup>/m<sup>3</sup> as a carrier. The treatment plan operated under four different organic load rate: OLR1 = 0.4 kg BOD/m<sup>3</sup>.day; OLR2 = 0.6 kg BOD/m<sup>3</sup>.day; OLR3 = 0.8 kg BOD/m<sup>3</sup>.day; and OLR4 = 1.0 kg BOD/m<sup>3</sup>.day. During 80 d of the experiment, the highest treatment efficiency was at the organic load rate of 0.4 kg BOD/m<sup>3</sup>.day, with COD, SS, TN and TP were found to be 85.0 ± 12.9%, 85.7 ± 5.3%, 68.9 ± 1.7%, and 40.3 ± 0.2%, respectively. In which, the influent SS concentration were from 117.3 to 126.0 mg/L, the effluent concentration were in ranged 18.0 to 34.22 mg/L, respectively. The values of influent and effluent COD were 298.8 ± 12.88 and 44.8 ± 3.78 mg/L in turn. The OLR1 influent TN, TP concentrations were respectively 47.9 ± 2.11 and 3.6 ± 0.15 mg/L; the effluent TN, TP concentration were 14.9 ± 0.18 and 2.2 ± 0.06 mg/L, respectively. The study suggests that the effluent is within the allowable limits of National technical regulation on domestic wastewater (Column B1), indicating the applicability of S-MBBR for the domestic wastewater treatment plant.

scholarly journals Impact of Operational Parameters on Bacterial Community in a Full-Scale Municipal Wastewater Treatment Plant

2012 ◽  
Vol 61 (1) ◽  
pp. 41-49 ◽  
Author(s):  
AGNIESZKA CYDZIK-KWIATKOWSKA ◽  
MAGDALENA ZIELIŃSKA ◽  
IRENA WOJNOWSKA-BARYŁA
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Organic Load ◽  
Municipal Wastewater Treatment ◽  
Load Rate ◽  
Organic Load Rate ◽  
Ammonia Oxidising Bacteria

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9-21.6 degrees C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD x g TSS(-1) x d(-1) were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9 degrees C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0:02) and organic load rate (R = -0.55, p = 0.04).

Production of poly-3-hydroxybutyrate from mixed culture

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Thomas Shalin ◽  
Raveendran Sindhu ◽  
Ashok Pandey ◽  
Vincenza Faraco ◽  
Parameswaran Binod
Keyword(s):  
Mixed Culture ◽  
Biodegradable Polymers ◽  
Biodegradable Polymer ◽  
The Other ◽  
Cost Difference ◽  
Microbial Cultures ◽  
Phb Production ◽  
Mixed Microbial Cultures ◽  
Main Barrier ◽  
The Cost

AbstractPoly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed.

Carbon and nitrogen removal from a wastewater of an industrial dairy laboratory with a coupled anaerobic filter-sequencing batch reactor system

2001 ◽  
Vol 43 (3) ◽  
pp. 249-256 ◽  
Author(s):  
J. M. Garrido ◽  
F. Omil ◽  
B. Arrojo ◽  
R. Méndez ◽  
J. M. Lema
Keyword(s):  
Nitrogen Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Treatment System ◽  
Organic Load ◽  
Anaerobic Sludge ◽  
Milk Analysis ◽  
Methanogenic Activity ◽  
Anaerobic Filter ◽  
Final Effluent

A set of two reactors, an Anaerobic Filter (AF) of 12 m3 and a Sequencing Batch Reactor (SBR) of 28 m3, coupled in series, were used to treat the wastewaters from an industrial milk analysis laboratory. The characteristics of these effluents are similar to those discharged by dairy factories (average values around 10 kg COD/m3 and 0.20 kg N/m3). These wastewaters were produced as the result of the final mixture of the analysed milk samples, with a very high organic load, and other low strength effluents, such as sewage and other minor liquid streams generated in the laboratory. Two microbial growth inhibitors, sodium azide and chloramphenicol, were systematically added to the milk before its analysis. Preliminary results have shown that these compounds did apparently not inhibit the methanogenic activity of the anaerobic sludge. Toxicity determination, using the Microtox method, resulted in EC50 values for the wastewaters of 20 g/L, whereas the final effluent from the SBR was non toxic. A maximum OLR of 8 kg COD/m3·d was treated in the AF, being the maximum OLR in the SBR around 1.5-2 kg COD/m3·d. During operation, the soluble COD of the final effluent from the SBR was usually below 200 mg/L, and total nitrogen (mainly nitrate) below 10 mg N/L. Assimilation of nitrogen for growth and nitrification-denitrification were the main mechanisms of nitrogen removal from the wastewater. In the anaerobic system between 50-85% of the organic matter was converted into methane, being the remaining COD and most of the nitrogen removed in the suspended culture system. Overall COD removal in the treatment system was 98% and the nitrogen removal up to 99%. The combination of the AF and the SBR was advantageous resulting in a lower energy consumption and sludge generation in the treatment system.

Efficiency of Attached-Growth Sequencing Batch Reactor in the Treatment of Recycled Paper Mill Wastewater

2015 ◽  
Vol 74 (3) ◽  
Author(s):  
Mohd Hafizuddin Muhamad ◽  
Siti Rozaimah Sheikh Abdullah ◽  
Hassimi Abu Hasan
Keyword(s):  
Organic Matter ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Nitrogen Compound ◽  
Oxygen Demand ◽  
Paper Mill ◽  
Organic Load ◽  
Recycled Paper ◽  
Attached Growth ◽  
Paper Mill Effluent

In this study, an attached-growth bioreactor was operated using granular activated carbon (GAC) with additional biomass; and evaluatedits performance in the treatment of real recycled paper mill effluent at chemical oxygen demand (COD) level in the range of 800-1300 mg/L, a fixed hydraulic retention time of 24 hours and COD:N:P ratio of about 100:5:1. A laboratory-scale aerobic sequencing batch reactor (SBR) was used. The efficiency of this biological treatment processwas studiedover a 300-day period, in order to evaluate their performance, especially for the removal of nitrogen compound and of biodegradable organic matter. It has been found that this process was able to remove organic matter (expressed as COD; 91-99%) and turbidity (89-99%) almost completely and simultaneously; the removal of nitrogen (expressed as NH3-N; 70-94%), phosphorus (expressed as PO43-P; 42-71%), suspended solid (81-99%) and colour (72-91%) were sufficiently achieved. The overall performance confirmed that an attached-growth SBR system using additional biomass on GAC is a promising configuration for wastewater treatment in terms of the performance efficiency and process stability under fluctuations of organic load.

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