Global expression profiling ofBacillus subtilis cells during industrial-close fed-batch fermentations with different nitrogen sources

2005 ◽  
Vol 92 (3) ◽  
pp. 277-298 ◽  
Author(s):  
Britta Jürgen ◽  
Steffen Tobisch ◽  
Mogens Wümpelmann ◽  
Dirk Gördes ◽  
Andreas Koch ◽  
...  
Aquaculture ◽  
2005 ◽  
Vol 243 (1-4) ◽  
pp. 217-224 ◽  
Author(s):  
D. Soletto ◽  
L. Binaghi ◽  
A. Lodi ◽  
J.C.M. Carvalho ◽  
A. Converti

1994 ◽  
Vol 16 (6) ◽  
pp. 575-580
Author(s):  
Hideo Tsujimura ◽  
Masamitsu Takaya ◽  
Kenji Katano ◽  
Nobuya Matsumoto ◽  
Yong Soo Park ◽  
...  

2002 ◽  
Vol 74 (5) ◽  
pp. 709-709
Author(s):  
B. Jürgen ◽  
M. Wümpelmann ◽  
S. Tobisch ◽  
C. Arndt ◽  
M. Hecker ◽  
...  

2013 ◽  
Vol 6 (3) ◽  
pp. 1118-1125 ◽  
Author(s):  
Raquel Pedrosa Bezerra ◽  
Marcelo Chuei Matsudo ◽  
Sunao Sato ◽  
Attilio Converti ◽  
João Carlos Monteiro de Carvalho

2019 ◽  
Vol 37 (No. 5) ◽  
pp. 383-390 ◽  
Author(s):  
Tomáš Humhal ◽  
Olga Kronusová ◽  
Petr Kaštánek ◽  
Tomáš Potočár ◽  
Jana Kohoutková ◽  
...  

An experimental design was ued to optimize the growth of two thraustochytrids, (Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4), on different nitrogen sources (yeast extract, corn steep liquor, ammonium sulphate) supplemented into saline waste water from the demineralization of cheese whey. Yeast extract was found to be the most suitable complex nutrient source. Nitrogen limitation was found to increase the lipid content in shake flask cultures of thraustochytrids by 12.7–22.4% w/w. The maximum total lipid content (79.1% w/w) and docosahexaenoic acid productivity (0.465 g/l per day) were achieved by J. marinum AN-4 in shake flask cultures. Fed-batch cultures of J. marinum AN-4, under conditions of nitrogen limitation, yielded biomass with a lower lipid content (72.1% wt.) but higher docosahexaenoic acid productivity (1.43 g/l per day). These results provide proof of concept that fed-batch cultivation of thraustochytrids, combined with nitrogen limitation, can be an appropriate strategy for the productive use of saline waste water from the dairy industry.


1995 ◽  
Vol 41 (13) ◽  
pp. 207-215 ◽  
Author(s):  
Sang Yup Lee ◽  
Ho Nam Chang

A number of Escherichia coli strains including K12, B, W, XL1-Blue, DH5α, HB101, JM109, and C600 were transformed with the stable high-copy-number plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyalkanoic acid biosynthesis genes, and were subsequently compared for their ability to synthesize and accumulate poly(3-hydroxybutyric acid) (PHB). The rate of PHB synthesis, the extent of PHB accumulation, and PHB yield from glucose varied considerably from one strain to another. Strains XL1-Blue and B harboring pSYL105 synthesized PHB at the highest rate to a final concentration of ca. 7 g/L in complex medium containing 20 g glucose/L. With an aim to reduce the cost of the medium, the effect on PHB accumulation of supplementing a defined medium with complex nitrogen sources was examined. A PHB concentration of 81 g/L could be obtained in 41 h from a pH-stat fed-batch culture of XL1-Blue(pSYL105) in a semidefined medium. When the availability of acetyl-CoA was increased by supplementing the medium with complex nitrogen sources, amino acids, or oleic acid, PHB synthesis by recombinant E. coli was enhanced.Key words: polyhydroxyalkanoic acid, poly(3-hydroxybutyric acid), fed batch, Escherichia coli.


2013 ◽  
Vol 50 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Z. Y. Zheng ◽  
Y. Jiang ◽  
X. B. Zhan ◽  
L. W. Ma ◽  
J. R. Wu ◽  
...  
Keyword(s):  

Sign in / Sign up

Export Citation Format

Share Document