Quantitative and kinetic study of oxidative stress regulons using green fluorescent protein

2005 ◽  
Vol 89 (5) ◽  
pp. 574-587 ◽  
Author(s):  
Canghai Lu ◽  
C. Renee Albano ◽  
William E. Bentley ◽  
Govind Rao
Keyword(s):  
Oxidative Stress ◽  
Green Fluorescent Protein ◽  
Kinetic Study ◽  
Fluorescent Protein ◽  
Green Fluorescent

High Throughput Studies of Gene Expression Using Green Fluorescent Protein-Oxidative Stress Promoter Probe Constructs The Potential for Living Chips

2001 ◽  
Vol 6 (6) ◽  
pp. 421-428
Author(s):  
C. Renee Albano ◽  
Canghai Lu ◽  
William E. Bentley ◽  
Govind Rao
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Green Fluorescent Protein ◽  
High Throughput ◽  
High Throughput Screening ◽  
Fluorescent Protein ◽  
Chip Technology ◽  
Great Specificity ◽  
Green Fluorescent ◽  
Stress Promoter

Green fluorescent protein fusions were constructed with several oxidative stress promoters from Escherichia coli. These promoters were chosen for their induction by reactive oxygen species (ROS) such as superoxide, hydrogen peroxide, and hydroxyl radicals. When exposed to various free radical insults, the cells fluoresced with great specificity based on the corresponding ROS. In this work, we propose a way in which these constructs could be used to study the mode of action of a variety of antitumor drugs. This approach offers the possibility of complementing gene chip technology by the creation of living chips for high throughput screening as well as studying differential gene expression.

Eel green fluorescent protein is associated with resistance to oxidative stress

2016 ◽  
Vol 181-182 ◽  
pp. 35-39 ◽  
Author(s):  
Aki Funahashi ◽  
Masaharu Komatsu ◽  
Tatsuhiko Furukawa ◽  
Yuki Yoshizono ◽  
Hikari Yoshizono ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

Monitoring Oxidative Stress and DNA Damage Induced by Heavy Metals in Yeast Expressing a Redox-Sensitive Green Fluorescent Protein

2009 ◽  
Vol 58 (5) ◽  
pp. 504-510 ◽  
Author(s):  
Shanshan Yu ◽  
Wei Qin ◽  
Guoqiang Zhuang ◽  
Xianen Zhang ◽  
Guanjun Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Dna Damage ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals A single-wavelength flow cytometric approach using redox-sensitive green fluorescent protein probes for measuring redox stress in live cells

BioTechniques ◽  
2021 ◽  
Author(s):  
Elizabeth R Denn ◽  
Joseph M Schober
Keyword(s):  
Oxidative Stress ◽  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Fluorescent Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Recovery Phase ◽  
Live Cells ◽  
Redox Stress ◽  
Green Fluorescent

Cellular redox changes are common in apoptosis, immune function, signaling pathways and cancer. The authors aimed to develop a single-wavelength method using the superior fluorescence sensitivity of a flow cytometer for measuring redox-sensitive green fluorescent protein signal during oxidative stress in cell lines. The single-wavelength method was able to discern small differences in oxidative stress between cell lines and between the cytoplasmic and mitochondrial compartments within the same cell line. In Chinese hamster ovary cells, the mitochondrial matrix compartment was more sensitive to oxidative stress compared with MDA-MB-231 cells, and the rapid changes in redox state were followed by a slow recovery phase. The authors conclude that this simplified method is useful and preferred for studies where alterations in overall redox-sensitive green fluorescent protein expression are controlled.

scholarly journals A Toxic Synergy between Aluminium and Amyloid Beta in Yeast

2021 ◽  
Vol 22 (4) ◽  
pp. 1835
Author(s):  
Jamieson B. Mcdonald ◽  
Sudip Dhakal ◽  
Ian Macreadie
Keyword(s):  
Oxidative Stress ◽  
Green Fluorescent Protein ◽  
Growth Inhibition ◽  
Cell Viability ◽  
Amyloid Beta ◽  
Fluorescent Protein ◽  
Late Onset ◽  
Model Organism ◽  
Age Related ◽  
Green Fluorescent

Alzheimer’s disease (AD), the most prevalent, age-related, neurodegenerative disease, is associated with the accumulation of amyloid beta (Aβ) and oxidative stress. However, the sporadic nature of late-onset AD has suggested that other factors, such as aluminium may be involved. Aluminium (Al3+) is the most ubiquitous neurotoxic metal on earth, extensively bioavailable to humans. Despite this, the link between Al3+ and AD has been debated for decades and remains controversial. Using Saccharomyces cerevisiae as a model organism expressing Aβ42, this study aimed to examine the mechanisms of Al3+ toxicity and its interactions with Aβ42. S. cerevisiae cells producing Aβ42 treated with varying concentrations of Al3+ were examined for cell viability, growth inhibition, and production of reactive oxygen species (ROS). Al3+ caused a significant reduction in cell viability: cell death in yeast producing green fluorescent protein tagged with Aβ42 (GFP–Aβ42) was significantly higher than in cells producing green fluorescent protein (GFP) alone. Additionally, Al3+ greatly inhibited the fermentative growth of yeast producing GFP–Aβ42, which was enhanced by ferric iron (Fe3+), while there was negligible growth inhibition of GFP cells. Al3+- induced ROS levels in yeast expressing native Aβ42 were significantly higher than in empty vector controls. These findings demonstrate Al3+ has a direct, detrimental toxic synergy with Aβ42 that can be influenced by Fe3+, causing increased oxidative stress. Thus, Al3+ should be considered as an important factor, alongside the known characteristic hallmarks of AD, in the development and aetiology of the disease.

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