Protein production and induction of the unfolded protein response inTrichoderma reeseistrain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tag

2004 ◽  
Vol 89 (3) ◽  
pp. 335-344 ◽  
Author(s):  
Anna Collén ◽  
Markku Saloheimo ◽  
Michael Bailey ◽  
Merja Penttilä ◽  
Tiina M. Pakula
Keyword(s):  
Unfolded Protein Response ◽  
Protein Production ◽  
Unfolded Protein ◽  
Endoglucanase I ◽  
The Unfolded Protein Response ◽  
Protein Response ◽  
Rut C30

scholarly journals Improvement of Foreign-Protein Production in Aspergillus niger var. awamori by Constitutive Induction of the Unfolded-Protein Response

2003 ◽  
Vol 69 (12) ◽  
pp. 6979-6986 ◽  
Author(s):  
Mari Valkonen ◽  
Michael Ward ◽  
Huaming Wang ◽  
Merja Penttilä ◽  
Markku Saloheimo
Keyword(s):  
Aspergillus Niger ◽  
Unfolded Protein Response ◽  
Protein Production ◽  
Production Systems ◽  
Foreign Protein ◽  
Mrna Levels ◽  
Unfolded Protein ◽  
Novel Strategy ◽  
The Unfolded Protein Response ◽  
Protein Response

ABSTRACT Unfolded-protein response (UPR) denotes the upregulation of endoplasmic reticulum (ER)-resident chaperone and foldase genes and numerous other genes involved in secretory functions during the accumulation of unfolded proteins into the ER. Overexpression of individual foldases and chaperones has been used in attempts to improve protein production in different production systems. We describe here a novel strategy to improve foreign-protein production. We show that the constitutive induction of the UPR pathway in Aspergillus niger var. awamori can be achieved by expressing the activated form of the transcription factor hacA. This induction enhances the production of Trametes versicolor laccase by up to sevenfold and of bovine preprochymosin by up to 2.8-fold in this biotechnically important fungus. The regulatory range of UPR was studied by analyzing the mRNA levels of novel A. niger var. awamori genes involved in different secretory functions. This revealed both similarities and differences to corresponding studies in Saccharomyces cerevisiae.

scholarly journals Effects of Inactivation and Constitutive Expression of the Unfolded- Protein Response Pathway on Protein Production in the Yeast Saccharomyces cerevisiae

2003 ◽  
Vol 69 (4) ◽  
pp. 2065-2072 ◽  
Author(s):  
Mari Valkonen ◽  
Merja Penttilä ◽  
Markku Saloheimo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Unfolded Protein Response ◽  
Protein Production ◽  
Active Form ◽  
Constitutive Expression ◽  
Amylase Secretion ◽  
Unfolded Protein ◽  
Foreign Proteins ◽  
The Unfolded Protein Response ◽  
Protein Response

ABSTRACT One strategy to obtain better yields of secreted proteins has been overexpression of single endoplasmic reticulum-resident foldases or chaperones. We report here that manipulation of the unfolded-protein response (UPR) pathway regulator, HAC1, affects production of both native and foreign proteins in the yeast Saccharomyces cerevisiae. The effects of HAC1 deletion and overexpression on the production of a native protein, invertase, and two foreign proteins, Bacillus amyloliquefaciens α-amylase and Trichoderma reesei endoglucanase EGI, were studied. Disruption of HAC1 caused decreases in the secretion of both α-amylase (70 to 75% reduction) and EGI (40 to 50% reduction) compared to the secretion by the parental strain. Constitutive overexpression of HAC1 caused a 70% increase in α-amylase secretion but had no effect on EGI secretion. The invertase levels were twofold higher in the strain overexpressing HAC1. Also, the effect of the active form of T. reesei hac1 was tested in S. cerevisiae. hac1 expression caused a 2.4-fold increase in the secretion of α-amylase in S. cerevisiae and also slight increases in invertase and total protein production. Overexpression of both S. cerevisiae HAC1 and T. reesei hac1 caused an increase in the expression of the known UPR target gene KAR2 at early time points during cultivation.

Oestrogen-driven changes in the bovine uterus are associated with activation of the unfolded protein response

2014 ◽  
Author(s):  
Mohammed A Alfattah ◽  
Paul Anthony McGettigan ◽  
John Arthur Browne ◽  
Khalid M Alkhodair ◽  
Katarzyna Pluta ◽  
...  
Keyword(s):  
Unfolded Protein Response ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response
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