Toward consistent and productive complex media for industrial fermentations: Studies on yeast extract for a recombinant yeast fermentation process

2003 ◽  
Vol 82 (6) ◽  
pp. 640-652 ◽  
Author(s):  
Jinyou Zhang ◽  
Jayanthi Reddy ◽  
Barry Buckland ◽  
Randolph Greasham
Keyword(s):  
Yeast Extract ◽  
Fermentation Process ◽  
Yeast Fermentation ◽  
Recombinant Yeast ◽  
Complex Media

scholarly journals Extraction and characterization of yeast extract bioethanol byproduct from empty palm oil bunch for raw material of cosmetic products

2018 ◽  
Vol 67 ◽  
pp. 03040
Author(s):  
Muhamad Sahlan ◽  
Muhammad Saefuddin ◽  
Muryanto ◽  
Heri Hermansyah ◽  
Anondho Wijanarko
Keyword(s):  
Yeast Extract ◽  
Raw Materials ◽  
Raw Material ◽  
Yeast Fermentation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chromatography Method ◽  
Column Adsorption ◽  
Liquid Chromatography Method ◽  
Feasibility Test ◽  
Placental Extract

Ethanolic fermentation can produce byproducts such as yeast containing intracellular amino acid that is used as a raw material of cosmetics. Residual yeast fermentation as sludge was dissolved and extracted by autolysis at 50°C for 24 hours, so we get the product in the form of intracellular content of the yeast Saccharomyces cerevisiae. Purification of dye and odor yeast extract was conducted by using an activated carbon column adsorption with ratio 1.5:10 yeast extract solution (g / mL) for six times recycle or until it reaches the absorbance value of 0.020. The content of yeast extract in the form of amino acids was analyzed by High-Pressure Liquid Chromatography method. Analysis of the feasibility test yeast extract as cosmetic raw materials made through the pigment deposition method by inhibit tyrosinase activity. 0.05 g yeast extract before adsorption (pale yellow) produce 62% inhibition of tyrosinase 3130 U / mL. Dry yeast extract after adsorption (odorless) had 96% inhibition of tyrosinase 313 U / mL, whereas placental extract by 89% inhibition of tyrosinase 313 U / mL. These results indicate odorless yeast extract can replace placental extract as an alternative to cosmetic raw materials.

In vitro growth of wheat and flax rust fungi on complex and chemically defined media

1974 ◽  
Vol 52 (6) ◽  
pp. 1183-1195 ◽  
Author(s):  
A. Bose ◽  
Michael Shaw
Keyword(s):  
Liquid Medium ◽  
Aspartic Acid ◽  
Yeast Extract ◽  
Australian Isolate ◽  
Puccinia Graminis ◽  
Good Growth ◽  
Complex Media ◽  
Mineral Salts ◽  
Melampsora Lini

Growth from uredospores seeded in axenic culture is described for several races of Puccinia graminis Pers. f. sp. tritici (Erikss. and Henn.) and race 3 of Melampsora lini (Ehrenb.) Lév. on complex media containing peptone, yeast extract, and bovine serum albumin (BSA); and for an Australian isolate of Puccinia graminis, race 126-ANZ 6,7, and Melampsora lini, race 3, on chemically defined, liquid media.Of six North American isolates of Puccinia graminis only race 38 formed colonies approaching those of race 126-ANZ 6,7 in final size and general morphology on complex media. 5′AMP had no effect on the growth of 126-ANZ 6,7, but cyclic AMP inhibited growth after uredospore germination. Good growth and sporulation were obtained with 126-ANZ 6,7, but not with the other isolates tested, using a new, chemically defined liquid medium, sterilized by millipore filtration, and containing glucose, Czapek's minerals plus micronutrients, Ca2+, glucose and aspartic acid, glutathione, and cysteine. Uredospores produced in culture reinfected exposed mesophyll tissue, but not intact seedling leaves of wheat.Highly reproducible growth and sporulation of Melampsora lini, race 3, were obtained routinely on a solid medium containing Difco-Bacto agar, sucrose, Knop's minerals, micronutrients, yeast extract, peptone, and BSA. Vegetative cultures, capable of reinfecting the cut ends of surface-sterilized flax cotyledons, could be maintained indefinitely by subdivision before sporulation and transfer to the same medium minus BSA. Evidence is presented that BSA stimulated the development of colonies and the formation of uredospores. The mode of action of BSA is unknown, but it could not be replaced by putrescine.A new chemically defined, liquid medium containing sucrose, Knop's mineral salts, micronutrients, aspartic (or glutamic) acid, and cysteine supported the growth of colonies of Melampsora lini in a highly reproducible manner. The formation of uredospores and teliospores by these colonies was controlled by (a) the level of Ca2+ (as Ca(NO3)2∙4H2O), (b) the concentration of aspartic acid, and (c) the number of colonies per flask. At inoculum levels giving 40 to 60 colonies per flask, in media containing 8.5 mM Ca+ and 45 mM aspartic acid, uredospore formation occurred in 60 to 70% of the colonies. A decrease in the Ca2+ level to 4.25 mM, or a decrease in aspartic acid to 22.5 mM, or adjustment of the inoculum level to give about 10 colonies per flask each resulted in only infrequent sporulation. The uredospores produced in vitro infected intact, 1-week-old flax cotyledons in a normal manner.

Increase of multimodular unit functioning efficiency for agroengineering technologies

10.12737/1346 ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. 49-52 ◽  
Author(s):  
Белова ◽  
Maryana Belova ◽  
Зиганшин ◽  
Bulat Ziganshin
Keyword(s):  
Electromagnetic Radiation ◽  
Quality Factor ◽  
Fermentation Process ◽  
Delay Systems ◽  
Agricultural Product ◽  
Yeast Fermentation ◽  
Sweat Bee ◽  
The Third ◽  
Generator Unit ◽  
Functional Purpose

The article narrates about the laboratory samples using the energy of electromagnetic radiation at different wave lengths . On the basis of these units, a multimodular microwave unit is made, which consists of the generator unit with resonator chambers, chambers and mechanisms for the threading process. One unit is assembled from four removable modules. The first module is designed to defrost the dough and flour products, the second is for the sweat bee-wax, and the third - for the pasteurization of milk, melange and melting out the melted butter; the fourth - to activate the baker’s yeast fermentation process. The main difference between the working units of the aggregate consists of modules and versions of resonator chambers. It was founded the configuration, volume and quality factor of the resonator chambers for the processing of agricultural product, according to the functional purpose of the process and structure of the material providing threading process. Resonator chamber systematized as follows: 1 ) stationary, rotating and moving camera 2) with punching, without perforation through a gap for conveying the product , and 3) the content of delay systems (to equalize the pressure, temperature and humidity throughout the structure material); 4 ) with individual and shared shield frame and etc.

scholarly journals UTILIZATION OF RESIDUAL CARRAGEENAN EXTRACT FROM Eucheuma cottonii SEAWEED INTO BIOETHANOL

2020 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
Nia Yuliani ◽  
RTM Sutamiharja ◽  
Aditya Prihantara
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Research Method ◽  
Fermentation Process ◽  
Optimal Time ◽  
Yeast Fermentation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fermentation Time ◽  
Time Treatment ◽  
Hydrolysis Process ◽  
Eucheuma Cottonii

In the process of processing seaweed will produce residual waste from carrageenan extraction, and the residue still contain cellulose, lignin, hemicellulose, pectin, and other organic materials that can be processed into bioethanol. This research aimed to utilize the residual carrageenan extracted from seaweed Eucheuma cottonii into bioethanol. The research method includes acid hydrolysis process using 3% sulfuric acid at a temperature of 70-80oC for 30 minutes, followed by a fermentation process using yeast Saccharomyces cerevisiae with a ratio of 1: 0.006 for hydrolyzate and yeast, fermentation time treatment 1, 3, 6, 9 and 12 days at temperature 25o-30oC. Fermentate at 78oC, measured in degrees of acidity (pH), volume, and levels of bioethanol. The results showed that the residual carrageenan extract containing carbohydrates as un-extracted carrageenan was 5.01%, hemicellulose was 7.12%, cellulose was 0.96%, and lignin was 8.26%. The level of bioethanol produced from the residual carrageenan extraction was 2.57% and, the yield was 32.64% with a fermentation time of 6 days as the optimal time.

scholarly journals The study of the main impurities mature mash depending on the duration of fermentation, yeast alcohol race and used enzyme preparations

2020 ◽  
Vol 82 (3) ◽  
pp. 78-84
Author(s):  
N. V. Zueva ◽  
G. V. Agafonov ◽  
E. A. Novokshenova ◽  
A. N. Dolgov ◽  
A. E. Chusova
Keyword(s):  
Fermentation Process ◽  
Ethanol Yield ◽  
Yeast Cells ◽  
Yeast Fermentation ◽  
High Concentration ◽  
Amylolytic Enzymes ◽  
Enzyme Preparations ◽  
Seed Rate ◽  
Different Strains ◽  
Volatile Impurities

The paper studied the process of intensification of concentrated fermentation mash with account of the complex amylolytic enzymes, hemicellulase and proteolytic activities. We determined the dependence of ethanol yield, the content of volatile impurities, the content of reducing substances, depending on the different strains of yeast at a seed rate 15 million cells per 1 cm3 wort. To identify the parameters of the fermentation process the wort with an increased solids content, investigated the dynamics of accumulation of volatile impurities by varying the rate and duration of seeding yeast fermentation using 987-O5 race yeast. It revealed that the qualitative composition of impurities in the mash, the resulting wort of high-concentration depends both on the duration of fermentation, and from normal seeding yeast. Thus, depending on the duration of the fermentation process, the acetaldehyde content increases (from 613,17 mg/dm? to 54 h to 1724,6 mg/dm? to 72 hours), the amount of ethyl acetate is reduced (from 409,2 mg/dm? to 54 hours prior to 207 mg/dm? to 72 hours), the methanol content to 54 h at a rate of 15 million yeast seeding cells per 1 cm? of the wort amounted to 0,0043 mg/cm?, whereas by 72 h of fermentation at the same seed rate yeast – 0,0070 mg/dm?, the amount of 1-propanol and 1-butanol was reduced from 903,14 mg/cm? and 6,38 mg/cm? -54 h to 880 mg/cm? and 5,57 mg/cm? - to 72 hours, respectively. The minimum content of isobutanol independent of the duration of fermentation was 900-1100 mg/cm? at a seed rate 15 million yeast cells per 1 cm? wort izoamilola number increases from 1219,08 mg/dm? (54 hr) to 2673,84 mg/dm? (72 h). It revealed that the smallest total amount of impurities obtained by seed rate yeast 15 million cells per 1 cm? wort at the duration of fermentation 54 hours was found that maximum ethanol content in the mash with minimal accumulation therein volatiles corresponds embodiment:. Duration of fermentation - 54 hours at normally the problem of yeast cells – 15,0 million / cm? wort.

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