Kinetic analysis of high-concentration isopropanol biodegradation by a solvent-tolerant mixed microbial culture

2002 ◽  
Vol 78 (6) ◽  
pp. 708-713 ◽  
Author(s):  
Mark T. Bustard ◽  
Vissanu Meeyoo ◽  
Phillip C. Wright
Keyword(s):  
Kinetic Analysis ◽  
Microbial Culture ◽  
High Concentration ◽  
Mixed Microbial Culture ◽  
Solvent Tolerant

Kinetics of growth and multi substrate degradation by an indigenous mixed microbial culture isolated from a wastewater treatment plant in Guwahati, India

2008 ◽  
Vol 58 (5) ◽  
pp. 1101-1106
Author(s):  
Pichiah Saravanan ◽  
K. Pakshirajan ◽  
P. K. Saha
Keyword(s):  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Lag Phase ◽  
Microbial Culture ◽  
High Concentration ◽  
Mixed Microbial Culture ◽  
Phenol Biodegradation ◽  
Low Concentration ◽  
Culture Growth

An indigenous mixed culture of microorganisms, isolated from a sewage treatment plant, was investigated for its potential to simultaneously degrade phenol and m-cresol during its growth in batch shake flasks. 22 full factorial designs with the two substrates as the factors, at two different levels and two different initial concentration ranges, were employed to carry out the biodegradation experiments. For complete utilisation of phenol and m-cresol, the culture took a minimum duration of 21 hrs at their low concentration of 100 mg/L each, and a maximum duration of 187 hrs at high concentration of 600 mg/L each in the multisubstrate system. The biodegradation results also showed that the presence of phenol in low concentration range (100–300 mg/L did not inhibit m-cresol biodegradation; on the other hand, presence of m-cresol inhibited phenol biodegradation by the culture. Moreover, irrespective of the concentrations used, phenol was degraded preferentially and earlier than m-cresol. During the culture growth, a lag phase was observed above a combined concentration of 500 mg/L i.e., 200 mg/L m-cresol and 300 mg/L of phenol and above). Statistical analysis of the specific growth rate of the culture in the multisubstrate system was also performed in the form of ANOVA and Student ‘t’ test, which gave good interpretation in terms of main and interaction effects of the substrates.

Ammonium-oxidizing bacteria facilitate aerobic degradation of sulfanilic acid in activated sludge

2014 ◽  
Vol 70 (6) ◽  
pp. 1122-1128 ◽  
Author(s):  
Gang Chen ◽  
Maneesha P. Ginige ◽  
Anna H. Kaksonen ◽  
Ka Yu Cheng
Keyword(s):  
Activated Sludge ◽  
Oxygen Uptake Rate ◽  
Substrate Inhibition ◽  
Inhibitory Effect ◽  
Sulfanilic Acid ◽  
Ammonium Concentration ◽  
Microbial Culture ◽  
High Concentration ◽  
Mixed Microbial Culture ◽  
First Time

Sulfanilic acid (SA) is a toxic sulfonated aromatic amine commonly found in anaerobically treated azo dye contaminated effluents. Aerobic acclimatization of SA-degrading mixed microbial culture could lead to co-enrichment of ammonium-oxidizing bacteria (AOB) because of the concomitant release of ammonium from SA oxidation. To what extent the co-enriched AOB would affect SA oxidation at various ammonium concentrations was unclear. Here, a series of batch kinetic experiments were conducted to evaluate the effect of AOB on aerobic SA degradation in an acclimatized activated sludge culture capable of oxidizing SA and ammonium simultaneously. To account for the effect of AOB on SA degradation, allylthiourea was used to inhibit AOB activity in the culture. The results indicated that specific SA degradation rate of the mixed culture was negatively correlated with the initial ammonium concentration (0–93 mM, R2 = 0.99). The presence of AOB accelerated SA degradation by reducing the inhibitory effect of ammonium (≥10 mM). The Haldane substrate inhibition model was used to correlate substrate concentration (SA and ammonium) and oxygen uptake rate. This study revealed, for the first time, that AOB could facilitate SA degradation at high concentration of ammonium (≥10 mM) in an enriched activated sludge culture.

Characterization of Nostoc muscorum NCCU-442 Derived Poly-3- hydroxybutyrate (PHB) and Polyethylene Glycol (PEG) Blends

2020 ◽  
Vol 10 (3) ◽  
pp. 200-207
Author(s):  
Sabbir Ansari ◽  
Tasneem Fatma
Keyword(s):  
Thermal Properties ◽  
Polyethylene Glycol ◽  
Morphological Properties ◽  
Morphological Alteration ◽  
Nostoc Muscorum ◽  
Microbial Culture ◽  
Polymer Science ◽  
Mixed Microbial Culture ◽  
Scanning Calorimetry ◽  
Casting Technique

Background: Poly-3-hydroxybutyrate (PHB) has attracted much consideration as biodegradable biocompatible polymer. This thermoplastic polymer has comparable material properties to polypropylene. Materials with more valuable properties may result from blending, a common practice in polymer science. Objective: In this paper, blends of PHB (extracted from cyanobacterium Nostoc muscorum NCCU- 442 with polyethylene glycol (PEG) were investigated for their thermal, tensile, hydrophilic and biodegradation properties. Methods: Blends were prepared in different proportions of PHB/PEG viz. 100/0, 98/2, 95/5, 90/10, 80/20, and 70/30 (wt %) using solvent casting technique. Morphological properties were investigated by using Scanning Electron Microscopy (SEM). Differential scanning calorimetry and thermogravimetric analysis were done for thermal properties determination whereas the mechanical and hydrophilic properties of the blends were studied by means of an automated material testing system and contact angle analyser respectively. Biodegradability potential of the blended films was tested as percent weight loss by mixed microbial culture within 60 days. Results: The blends showed good misciblity between PEG and PHB, however increasing concentrations of plasticizer caused morphological alteration as evidenced by SEM micrographs. PEG addition (10 % and above) showed significant alternations in the thermal properties of the blends. Increase in the PEG content increased the elongation at break ratio i.e enhanced the required plasticity of PHB. Rate of microbial facilitated degradation of the blends was greater with increasing PEG concentrations. Conclusion: Blending with PEG increased the crucial polymeric properties of cyanobacterial PHB.

PHA accumulation of a mixed microbial culture co-exists with ammonia partial nitritation

2019 ◽  
Vol 360 ◽  
pp. 1255-1261 ◽  
Author(s):  
Andrea Fra-Vázquez ◽  
Sergio Santorio ◽  
Tania Palmeiro-Sánchez ◽  
Ángeles Val del Río ◽  
Anuska Mosquera-Corral
Keyword(s):  
Microbial Culture ◽  
Mixed Microbial Culture ◽  
Partial Nitritation

Screening and characterisation of a poly(vinyl alcohol)-degrading mixed microbial culture

2017 ◽  
Vol 62 (1) ◽  
pp. 17 ◽  
Author(s):  
Jie Zhang ◽  
Ying Zhang ◽  
Xuerong Fan ◽  
Ping Wang ◽  
Artur Cavaco Paulo ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Microbial Culture ◽  
Mixed Microbial Culture

Characteristics of mixed microbial culture at different sludge ages: Effect on variable kinetics for substrate utilization

2012 ◽  
Vol 126 ◽  
pp. 274-282 ◽  
Author(s):  
Ilke Pala-Ozkok ◽  
Ateequr Rehman ◽  
Nevin Yagci ◽  
Emine Ubay-Cokgor ◽  
Daniel Jonas ◽  
...  
Keyword(s):  
Substrate Utilization ◽  
Microbial Culture ◽  
Mixed Microbial Culture
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