A microfabricated array bioreactor for perfused 3D liver culture

2002 ◽  
Vol 78 (3) ◽  
pp. 257-269 ◽  
Author(s):  
Mark J. Powers ◽  
Karel Domansky ◽  
Mohammad R. Kaazempur-Mofrad ◽  
Artemis Kalezi ◽  
Adam Capitano ◽  
...  
Keyword(s):  
Liver Culture

Organotypic liver culture in a fluid-air interface using slices of neonatal rat and adult human tissue—a model of fibrosis in vitro

2002 ◽  
Vol 48 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Clare Verrill ◽  
Janice Davies ◽  
Harry Millward-Sadler ◽  
Lars Sundstrom ◽  
Nick Sheron
Keyword(s):  
Human Tissue ◽  
Neonatal Rat ◽  
Adult Human ◽  
Air Interface ◽  
Liver Culture

scholarly journals Organotypic liver culture models: Meeting current challenges in toxicity testing

2012 ◽  
Vol 42 (6) ◽  
pp. 501-548 ◽  
Author(s):  
Edward L. LeCluyse ◽  
Rafal P. Witek ◽  
Melvin E. Andersen ◽  
Mark J. Powers
Keyword(s):  
Toxicity Testing ◽  
Liver Culture ◽  
Culture Models

scholarly journals In vitro propagation of parvovirus B19 in primary foetal liver culture

1991 ◽  
Vol 72 (3) ◽  
pp. 741-745 ◽  
Author(s):  
K. E. Brown ◽  
J. Mori ◽  
B. J. Cohen ◽  
A. M. Field
Keyword(s):  
In Vitro Propagation ◽  
Parvovirus B19 ◽  
Liver Culture ◽  
Foetal Liver

scholarly journals Technical advances in the development of zonation liver in vitro systems that incorporate localized Wnt activating signals

2021 ◽  
Author(s):  
Eider Valle-Encinas ◽  
Michael Dawes ◽  
Carmen Velasco Martinez ◽  
Kate McSweeney ◽  
Miryam Muller ◽  
...  
Keyword(s):  
Bile Duct ◽  
Central Vein ◽  
Functional Heterogeneity ◽  
Culture Systems ◽  
Liver Culture ◽  
Liver Functions ◽  
Technical Advances ◽  
In Vitro Systems ◽  
Presentation Methods

A Wnt microenvironment sustained by the hepatic central vein is essential for the segregation of liver functions into zones. Current liver culture systems lack localized Wnt cues and as a consequence fail to maintain the hepatocyte functional heterogeneity that is observed in the intact organ. In this study, organoid models and 2D-culture systems were used to identify cellular sources and Wnt presentation methods that could support the future development of zonated liver in vitro systems. Using soluble ligands, we show that primary hepatocyte (PH)-derived organoids but not bile duct (BD)-derived organoids may be used to recapitulate the resting liver. We provide evidence that differentiation of PH-organoids in the presence of Wnt9b and Rspo3 induce pericentral maturation. Finally, we show that immobilization of Rspo3 onto beads in combination with soluble Wnt9b may be a valid strategy to recreate the central vein Wnt microenvironment in vitro.

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