Random coil scission rates determined by time-dependent total intensity light scattering: Hyaluronate depolymerization by hyaluronidase

Biopolymers ◽  
1990 ◽  
Vol 30 (11-12) ◽  
pp. 1073-1082 ◽  
Author(s):  
Wayne F. Reed ◽  
Christopher E. Reed ◽  
Larry D. Byers
1970 ◽  
Vol 23 (9) ◽  
pp. 1801 ◽  
Author(s):  
JH Bradbury ◽  
BE Chapman

Poly-L-methionine samples have been produced by polymerization of L- methionine-N-carboxyanhydride and their properties studied by light scattering and viscometry in organic solvents. In trifluoroacetic acid viscosity studies reveal a polyelectrolyte effect, hence the poly-L-methionine is charged. Furthermore, it is a random coil and fits the same Mark-Houwink equation as does poly-γ-benzyl-L-glutamate in dichloroacetic acid. Poly-L-methionine is a helical rod in ethylene dichloride + 2% dichloroacetic acid and shows the same viscometric behaviour as poly-y-benzyl-L-glutamate in dimethylformamide. Comparison of five different organic soluble polypeptides shows that there is an approximate correlation between the rigidity of the helix and its stability.


2006 ◽  
Author(s):  
Vladimir A. Bogatyrev ◽  
Lev A. Dykman ◽  
Anna V. Alekseeva ◽  
Boris N. Khlebtsov ◽  
Anna P. Novikova ◽  
...  

1971 ◽  
Vol 25 (5) ◽  
pp. 509-516 ◽  
Author(s):  
C. C. Gravatt

Light scattering provides information concerning the size, shape, number, and time dependence of the physical nonuniformities of a system. Measurements of the scattered intensity as a function of both scattering angle and wavelength describe static properties, whereas the spectral distribution of the scattered radiation is related to time dependent phenomena. The applications of light scattering are discussed, including critical phenomena, molecular weight determination, air pollution analysis, and diffusion phenomena.


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