CD of ethidium bromide complexes with normal and electrophoretically anomalous DNA restriction fragments

Biopolymers ◽  
1988 ◽  
Vol 27 (7) ◽  
pp. 1107-1126 ◽  
Author(s):  
Elizabeth Slobodyansky ◽  
John Stellwagen ◽  
Nancy C. Stellwagen
Keyword(s):  
Ethidium Bromide ◽  
Restriction Fragments ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Characterization of Cloned Mitochondrial DNA from the Teleost Fundulus heteroclitus and its Usefulness as an Interspecies Hybridization Probe

1986 ◽  
Vol 43 (10) ◽  
pp. 1866-1872 ◽  
Author(s):  
Lucia Irene González-Villaseñor ◽  
Amanda M. Burkhoff ◽  
Víctor Corces ◽  
Dennis A. Powers
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Fundulus Heteroclitus ◽  
Staining Technique ◽  
Hybridization Probe ◽  
Cross Hybridization ◽  
Restriction Patterns ◽  
Ethidium Bromide Staining ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Analysis of mitochondrial DNA endonuclease restriction patterns is a powerful tool for studying related species and variation within species. The ethidium bromide staining technique has limited the number of digestions of mitochondrial DNA per individual. Because 32P-end-labeling also imposes severe limitations, we have resorted to cloning the fish (Fundulus heteroclitus) mitochondrial genome in the lambda replacement vector EMBL-3. The clone was used as a radioactive probe via Southern blotting to detect mitochondrial DNA restriction fragments obtained by multiple restriction endonuclease digestions from small amounts of tissue. This technique offers much greater sensitivity than ethidium bromide staining. Moreover, it eliminates the expense and time to obtain highly purified mitochondrial DNA for the 32P-end-labeling procedure. It is also useful when the mtDNA is prepared from frozen tissue which has been a problem with the 32P-end-labeling technique. Because the cloned mitochondrial DNA has a high degree of cross-hybridization with the mitochondrial DNA of certain other fishes, it can be used to probe the mitochondrial DNA restriction patterns of a variety of fish species. However, its usefulness is restricted by the degree of relatedness to the species being cloned.

scholarly journals LOCALIZATION IN THE TOMATO GENOME OF DNA RESTRICTION FRAGMENTS CONTAINING SEQUENCES HOMOLOGOUS TO THE rRNA (45S), THE MAJOR CHLOROPHYLL a/b BINDING POLYPEPTIDE AND THE RIBULOSE BISPHOSPHATE CARBOXYLASE GENES

Genetics ◽  
1986 ◽  
Vol 112 (1) ◽  
pp. 93-105
Author(s):  
C E Vallejos ◽  
S D Tanksley ◽  
R Bernatzky
Keyword(s):  
Chlorophyll A ◽  
Ribosomal Rna ◽  
Chromosome 8 ◽  
Ribulose Bisphosphate Carboxylase ◽  
Chromosome 2 ◽  
Ribulose Bisphosphate ◽  
Bisphosphate Carboxylase ◽  
Restriction Fragments ◽  
Dna Restriction Fragments ◽  
Dna Restriction

ABSTRACT DNA restriction fragments containing sequences homologous to the ribosomal RNA (45s), the major chlorophyll a/b binding polypeptide (CAB) and the small subunit of ribulose bisphosphate carboxylase (RBCS) genes have been localized and mapped in the tomato nuclear genome by linkage analysis. Ribosomal RNA genes map to a single locus, R45s, which resides in a terminal position on the short arm of chromosome 2 and corresponds to the Nucleolar Organizer Region. The size of the 45s repeating unit is estimated to be approximately 9 kb in Lycopersicon esculentum and 11 kb in Lycopersicon pennellii. Five loci were found to contain CAB sequences. Two of the loci, Cab-1 (chromosome 2) and Cab-3 (chromosome 8), together accounted for more than 80% of the hybridization signal. These loci contain more than one CAB structural gene. The other three loci, Cab-2 (chromosome 8), Cab-4 (chromosome 7) and Cab-5 (chromosome 12), each account for <10% of the total signal and may contain only a single copy of the CAB structural sequence. Three loci were found to contain RBCS sequences. Rbcs-2 (chromosome 3) and Rbcs-3 (chromosome 2) were responsible for >80% of the signal, with the remainder being associated with Rbcs-1 (chromosome 2). Rbcs-2 and Rbcs-3 may contain more than one copy of the gene.

A sizing artifact of DNA restriction fragments with unusually long single-stranded termini

Gene ◽  
1991 ◽  
Vol 102 (1) ◽  
pp. 79-81 ◽  
Author(s):  
David M. Woodcock ◽  
Penelope J. Crowther ◽  
Judith P. Doherty ◽  
Martha E. Linsenmeyer ◽  
Detlev H. Krüger
Keyword(s):  
Restriction Fragments ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Separation of DNA restriction fragments by ion-pair chromatography

1986 ◽  
Vol 359 ◽  
pp. 265-274 ◽  
Author(s):  
Stig Eriksson ◽  
Gunnar Glad ◽  
Per-Åke Pernemalm ◽  
Elisabeth Westman
Keyword(s):  
Ion Pair ◽  
Restriction Fragments ◽  
Ion Pair Chromatography ◽  
Dna Restriction Fragments ◽  
Dna Restriction
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