Theoretical studies on protein-nucleic acid interactions. III. Stacking of aromatic amino acids with bases and base pairs of nucleic acids

Biopolymers ◽  
1984 ◽  
Vol 23 (10) ◽  
pp. 2009-2024 ◽  
Author(s):  
N. Vasant Kumar ◽  
Girjesh Govil
Keyword(s):  
Amino Acids ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Aromatic Amino Acids ◽  
Theoretical Studies ◽  
Base Pairs ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

Covalent Binding of Modified Nucleic Acids to Proteins as a Method for Investigation of Specific Protein—Nucleic Acid Interactions

ChemInform ◽  
2005 ◽  
Vol 36 (28) ◽  
Author(s):  
T. S. Zatsepin ◽  
N. G. Dolinnaya ◽  
E. A. Kubareva ◽  
M. G. Ivanovskaya ◽  
V. G. Metelev ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Covalent Binding ◽  
Specific Protein ◽  
Modified Nucleic Acids ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

Covalent binding of modified nucleic acids to proteins as a method for investigation of specific protein–nucleic acid interactions

2005 ◽  
Vol 74 (1) ◽  
pp. 77-95 ◽  
Author(s):  
Timofei S Zatsepin ◽  
N G Dolinnaya ◽  
Elena A Kubareva ◽  
Marina G Ivanovskaya ◽  
V G Metelev ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Covalent Binding ◽  
Specific Protein ◽  
Modified Nucleic Acids ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

Role of aromatic amino acids in protein-nucleic acid recognition

Biopolymers ◽  
2007 ◽  
Vol 85 (5-6) ◽  
pp. 456-470 ◽  
Author(s):  
Christopher M. Baker ◽  
Guy H. Grant
Keyword(s):  
Amino Acids ◽  
Nucleic Acid ◽  
Aromatic Amino Acids ◽  
Protein Nucleic Acid

Use of difference boundary sedimentation velocity to investigate nonspecific protein-nucleic acid interactions

Biochemistry ◽  
1980 ◽  
Vol 19 (15) ◽  
pp. 3516-3522 ◽  
Author(s):  
Timothy M. Lohman ◽  
C. Glen Wensley ◽  
Jeffrey Cina ◽  
Richard R. Burgess ◽  
M. Thomas Record
Keyword(s):  
Nucleic Acid ◽  
Sedimentation Velocity ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions
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