Centrifugal field relaxation and ionic strength effects on calf thymus DNA gels

Biopolymers ◽  
1983 ◽  
Vol 22 (3) ◽  
pp. 935-943 ◽  
Author(s):  
Alfred J. Richard
Keyword(s):  
Ionic Strength ◽  
Calf Thymus Dna ◽  
Centrifugal Field ◽  
Calf Thymus

DUPLEX–TRIPLEX TRANSITION IN COMPLEXES OF NUCLEIC ACIDS WITH HOECHST 33258

2021 ◽  
Vol 55 (1 (254)) ◽  
pp. 39-45
Author(s):  
Marine A. Parsadanyan
Keyword(s):  
Ionic Strength ◽  
Nucleic Acids ◽  
Calf Thymus Dna ◽  
Hoechst 33258 ◽  
Groove Binding ◽  
Melting Curves ◽  
Calf Thymus ◽  
High Concentrations

The study of complexes of groove binding ligand Hoechst 33258 (H33258) with Calf Thymus DNA has been carried out. The data obtained revealed that the melting curves of the complexes of H33258 with DNA are monophasic at low ligand concentrations (0 < r ≤ 0.2) and become biphasic at relatively high concentrations (0.2 < r ≤ 0.33). This effect was revealed to depend on the ionic strength of the solution, and can also occur at high concentrations of the ligand. Comparison of the obtained data with the results for poly(rA)-poly(rU) and poly(dA)-poly(dT) shows a coincidence in the case of DNA and poly(rA)-poly(rU), while in the case of poly(dA)-poly(dT) the melting curves become biphasic at low ligand concentrations and actually do not depend on the ionic strength of the solution.

Ionic Strength Dependence of the Binding of Methylene Blue to Chromatin and Calf Thymus DNA

1992 ◽  
Vol 9 (4) ◽  
pp. 667-679 ◽  
Author(s):  
Per Hagmar ◽  
Stefan Pierrou ◽  
Peter Nielsen ◽  
Bengt Nordén ◽  
Mikael Kubista
Keyword(s):  
Methylene Blue ◽  
Ionic Strength ◽  
Calf Thymus Dna ◽  
Ionic Strength Dependence ◽  
Calf Thymus ◽  
Strength Dependence

Spectroscopic and viscometric elucidation of the interaction between a potential chloride channel blocker and calf-thymus DNA: the effect of medium ionic strength on the binding mode

2015 ◽  
Vol 17 (1) ◽  
pp. 483-492 ◽  
Author(s):  
Aniruddha Ganguly ◽  
Soumen Ghosh ◽  
Nikhil Guchhait
Keyword(s):  
Ionic Strength ◽  
Chloride Channel ◽  
Channel Blocker ◽  
Binding Mode ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Detailed Characterization ◽  
Calf Thymus ◽  
Chloride Channel Blocker

The present study demonstrates a detailed characterization of the binding interaction of a potential chloride channel blocker 9-methyl anthroate (9-MA) with calf-thymus DNA.

Molecular Transformations in DNA under the Influence of UV-radiation

2020 ◽  
Vol 04 ◽  
Author(s):  
Vigen G. Barkhudaryan ◽  
Gayane V. Ananyan ◽  
Nelli H. Karapetyan
Keyword(s):  
Uv Radiation ◽  
Uv Irradiation ◽  
Absorption Spectroscopy ◽  
Calf Thymus Dna ◽  
Dilute Solutions ◽  
Concentrated Solutions ◽  
Buffer Solution ◽  
Low Concentration ◽  
Calf Thymus ◽  
Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

Ce(IV)-mediated formation of benzenediazonium ion from a non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) and its binding to DNA

1989 ◽  
Vol 54 (7) ◽  
pp. 2021-2026
Author(s):  
Marie Stiborová ◽  
Befekadu Asfaw ◽  
Pavel Anzenbacher
Keyword(s):  
Azo Dyes ◽  
Acidic Medium ◽  
Model System ◽  
Calf Thymus Dna ◽  
Suitable Model ◽  
Principal Product ◽  
Calf Thymus ◽  
Sudan I ◽  
A Minor

Ce(IV) ions in acidic medium convert a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) into an ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the Ce(IV) system is the benzenediazonium ion. A minor product is the dihydroxyderivative of Sudan I, 1-(4-hydroxyphenylazo)-2,6-dihydroxynaphthalene. Other minor coloured products (yellow and brown) were not identified. The principal product (the benzenediazonium ion) is responsible for the carcinogenicity of Sudan I, as it covalently binds to DNA. Ce(IV) ions in acidic medium represent a suitable model system, which imitates the activation route of carcinogenic azo dyes.

Investigation of Anti-Cancer Drug Nimustine Interaction with Calf Thymus DNA

MAPAN ◽  
2016 ◽  
Vol 31 (3) ◽  
pp. 169-175 ◽  
Author(s):  
Deepti Chadha ◽  
Shweta Agarwal ◽  
Ranjana Mehrotra
Keyword(s):  
Calf Thymus Dna ◽  
Cancer Drug ◽  
Calf Thymus ◽  
Anti Cancer

Studies on free radical scavenging, cancer cell antiproliferation, and calf thymus DNA interaction of Schiff bases

2017 ◽  
Vol 172 ◽  
pp. 129-138 ◽  
Author(s):  
Cleiton M. da Silva ◽  
Marina M. Silva ◽  
Fabiano S. Reis ◽  
Ana Lúcia T.G. Ruiz ◽  
João E. de Carvalho ◽  
...  
Keyword(s):  
Free Radical ◽  
Schiff Bases ◽  
Cancer Cell ◽  
Radical Scavenging ◽  
Dna Interaction ◽  
Free Radical Scavenging ◽  
Calf Thymus Dna ◽  
Calf Thymus
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