scholarly journals Ribosomal biosynthesis of the cyclic peptide toxins of Amanita mushrooms

Biopolymers ◽  
2010 ◽  
Vol 94 (5) ◽  
pp. 659-664 ◽  
Author(s):  
Jonathan D. Walton ◽  
Heather E. Hallen-Adams ◽  
Hong Luo
Keyword(s):  
Cyclic Peptide ◽  
Peptide Toxins ◽  
Amanita Mushrooms

scholarly journals Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach

2021 ◽  
Vol 7 (3) ◽  
pp. 204
Author(s):  
Shengwen Zhou ◽  
Xincan Li ◽  
Yunjiao Lüli ◽  
Xuan Li ◽  
Zuo H. Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cyclic Peptides ◽  
Cyclic Peptide ◽  
Amino Acid Residues ◽  
Fragment Ions ◽  
Peptide Pair ◽  
A Genome ◽  
Peptide Toxins ◽  
Poisonous Mushrooms ◽  
Large Peptide

Most species in the genus Amanita are ectomycorrhizal fungi comprising both edible and poisonous mushrooms. Some species produce potent cyclic peptide toxins, such as α-amanitin, which places them among the deadliest organisms known to mankind. These toxins and related cyclic peptides are encoded by genes of the “MSDIN” family (named after the first five amino acid residues of the precursor peptides), and it is largely unknown to what extent these genes are expressed in the basidiocarps. In the present study, Amanita rimosa and Amanita exitialis were sequenced through the PacBio and Illumina techniques. Together with our two previously sequenced genomes, Amanita subjunquillea and Amanita pallidorosea, in total, 46 previously unknown MSDIN genes were discovered. The expression of over 80% of the MSDIN genes was demonstrated in A. subjunquillea. Through a combination of genomics and mass spectrometry, 12 MSDIN genes were shown to produce novel cyclic peptides. To further confirm the results, three of the cyclic peptides were chemically synthesized. The tandem mass spectrometry (MS/MS) spectra of the natural and the synthetic peptides shared a majority of the fragment ions, demonstrating an identical structure between each peptide pair. Collectively, the results suggested that the genome-guided approach is reliable for identifying novel cyclic peptides in Amanita species and that there is a large peptide reservoir in these mushrooms.

scholarly journals CYCLIC PEPTIDE TOXINS CONTAINED IN THE STRAINS AND NATURAL SAMPLES OF MICROCYSTIS

1988 ◽  
Vol 1988 (1Supplement) ◽  
pp. 7-8 ◽  
Author(s):  
M.F. WATANABE ◽  
K.-I. HARADA ◽  
K. MATSUURA ◽  
S. OISHI ◽  
M. SUZUKI
Keyword(s):  
Cyclic Peptide ◽  
Peptide Toxins

scholarly journals Chemical Study of the Hepatotoxins from Microcystis Aeruginosa Collected in California

1993 ◽  
Vol 5 (3) ◽  
pp. 409-412 ◽  
Author(s):  
S. E. DeVries ◽  
M. Namikoshi ◽  
F. D. Galey ◽  
J. E. Merritt ◽  
K. L. Rinehart ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Microcystis Aeruginosa ◽  
High Performance ◽  
Fast Atom Bombardment ◽  
Cyclic Peptide ◽  
Algal Cell ◽  
Chemical Study ◽  
Blue Green Alga ◽  
Peptide Toxins ◽  
Layer Chromatography

Four cyclic peptide toxins were purified and quantified from the aqueous extract of algal cell material utilizing high performance liquid chromatography, thin layer chromatography, and fast atom bombardment mass spectrometry. The cyclic peptide toxins appear to be similar structurally to hepatotoxins Iron previously identified blooms of the blue-green alga Microcystis aeruginosa.

ChemInform Abstract: Engineering Cyclic Peptide Toxins

ChemInform ◽  
2012 ◽  
Vol 43 (38) ◽  
pp. no-no
Author(s):  
Richard J. Clark ◽  
David J. Craik
Keyword(s):  
Cyclic Peptide ◽  
Peptide Toxins

A Sensitive Bioscreen for Detection of Cyclic Peptide Toxins of the Microcystin Class

1994 ◽  
pp. 85-89 ◽  
Author(s):  
Charles F.B. Holmes ◽  
Tara L. McCready ◽  
Marcia Craig ◽  
Timothy W. Lambert ◽  
Steve E. Hrudey
Keyword(s):  
Cyclic Peptide ◽  
Peptide Toxins
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