Real-time detection of single-living pancreatic β-cell by laser tweezers Raman spectroscopy: High glucose stimulation

Biopolymers ◽  
2010 ◽  
Vol 93 (7) ◽  
pp. 587-594 ◽  
Author(s):  
Xi Rong ◽  
Shu-Shi Huang ◽  
Xiao-Cong Kuang ◽  
Hong Liu
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
High Glucose ◽  
Laser Tweezers ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Glucose Stimulation ◽  
Real Time Detection ◽  
Single Living

Real-Time Detection on the Expression of Soluble Protein and Inclusion Body in the Recombinant Escherichia coli with Laser Tweezers Raman Spectroscopy

2014 ◽  
Vol 41 (12) ◽  
pp. 1215003
Author(s):  
黄庶识 Huang Shushi ◽  
卢明倩 Lu Mingqian ◽  
李冰 Li Bing ◽  
周冰 Zhou Bing ◽  
陈丽梅 Chen Limei
Keyword(s):  
Escherichia Coli ◽  
Raman Spectroscopy ◽  
Real Time ◽  
Inclusion Body ◽  
Soluble Protein ◽  
Recombinant Escherichia Coli ◽  
Laser Tweezers ◽  
Real Time Detection

Honokiol protects pancreatic β cell against high glucose and intermittent hypoxia-induced injury by activating Nrf2/ARE pathway in vitro and in vivo

2018 ◽  
Vol 97 ◽  
pp. 1229-1237 ◽  
Author(s):  
Chen-guang Li ◽  
Chang-lin Ni ◽  
Min Yang ◽  
Yun-zhao Tang ◽  
Zhu Li ◽  
...  
Keyword(s):  
High Glucose ◽  
Intermittent Hypoxia ◽  
Pancreatic Β Cell ◽  
Β Cell

scholarly journals Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

2011 ◽  
Vol 35 (5) ◽  
pp. 469 ◽  
Author(s):  
Eun-Mi Lee ◽  
Young-Eun Lee ◽  
Esder Lee ◽  
Gyeong Ryul Ryu ◽  
Seung-Hyun Ko ◽  
...  
Keyword(s):  
Protective Effect ◽  
Heme Oxygenase ◽  
High Glucose ◽  
Cell Injury ◽  
Heme Oxygenase 1 ◽  
Pancreatic Β Cell ◽  
Β Cell

Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis

2006 ◽  
Vol 11 (2) ◽  
pp. 024011 ◽  
Author(s):  
Juqiang Lin ◽  
Zhihong Zhang ◽  
Jie Yang ◽  
Shaoqun Zeng ◽  
Bi-Feng Liu ◽  
...  
Keyword(s):  
Hela Cell ◽  
Real Time ◽  
Real Time Detection ◽  
Caspase 2 ◽  
Induced Apoptosis ◽  
Single Living

Real-time detection of early-stage calcium sulfate and calcium carbonate scaling using Raman spectroscopy

2020 ◽  
Vol 596 ◽  
pp. 117603 ◽  
Author(s):  
Omkar D. Supekar ◽  
Danielle J. Park ◽  
Alan R. Greenberg ◽  
Juliet T. Gopinath ◽  
Victor M. Bright
Keyword(s):  
Raman Spectroscopy ◽  
Calcium Carbonate ◽  
Real Time ◽  
Calcium Sulfate ◽  
Early Stage ◽  
Real Time Detection

scholarly journals Real-Time Detection of Concealed Chemical Hazards Under Ambient Light Conditions Using Raman Spectroscopy

2013 ◽  
Vol 58 (4) ◽  
pp. 1008-1014 ◽  
Author(s):  
Biju Cletus ◽  
William Olds ◽  
Peter M. Fredericks ◽  
Esa Jaatinen ◽  
Emad L. Izake
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Ambient Light ◽  
Light Conditions ◽  
Chemical Hazards ◽  
Real Time Detection

scholarly journals Real-time detection of ?1A-AR movement stimulated by phenylephrine in single living cells

2007 ◽  
Vol 28 (6) ◽  
pp. 796-802 ◽  
Author(s):  
Ning XU ◽  
Zhang-yi LIANG ◽  
Ming XU ◽  
Ying-hua GUAN ◽  
Qi-hua HE ◽  
...  
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Real Time Detection ◽  
Single Living Cells ◽  
Single Living

scholarly journals Glucose stimulation of protein acylation in the pancreatic β-cell

Life Sciences ◽  
2010 ◽  
Vol 87 (23-26) ◽  
pp. 667-671 ◽  
Author(s):  
Mossaad Abdel-Ghany ◽  
Geoffrey W.G. Sharp ◽  
Susanne G. Straub
Keyword(s):  
Pancreatic Β Cell ◽  
Β Cell ◽  
Glucose Stimulation ◽  
Protein Acylation ◽  
Stimulation Of

Laser Tweezers Raman Spectroscopy As a Novel Red Blood Cell Functional Assay for Sickle Cell Disease

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4847-4847 ◽  
Author(s):  
Rui Liu ◽  
Lena Zheng ◽  
Denis M. Dwyre ◽  
Fabrizia Urbinati ◽  
Donald B. Kohn ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Oxygen Content ◽  
Living Cells ◽  
Laser Tweezers ◽  
Functional Assay ◽  
Optical Forces ◽  
Cell Disease ◽  
Single Living Cells ◽  
Single Living ◽  
The U.S

Abstract Abstract 4847 The number of individuals with sickle cell disease (SCD) in the U.S. is estimated to be over 70,000, and the average life span of a SCD patient is 39 years. Despite the high incidence of SCD in the U.S. and its significant negative effects on patient health, there are currently only limited treatment options for this disease. Also, although a single mutation in the globin gene is known to cause SCD, there is significant clinical diversity. As technology advances, many studies, including genome-wide analyses and proteomics, have been conducted to understand the disease mechanism and find better treatments. However, none has yet led to the development of novel therapies. We need a practical approach to study red blood cells (RBCs) to increase our knowledge of this heterogeneous disease. Laser tweezers Raman spectroscopy (LTRS) is a label-free, laser-based method for the biochemical analysis of single living cells. Based on the phenomenon of inelastic light scattering of photons by molecular bonds, Raman spectroscopy can provide detailed information, in the form of a Raman spectrum, about the molecular structure and conformations inside single living cells. Moreover, the integration of laser tweezers enables 1) simple, convenient analysis of single suspension cells such as RBCs and 2) a method to impart a mechanical force on a cell (via the optical forces) and simultaneously monitor the biochemical response of that cell to the applied force. Previous studies using optical tweezers have shown different RBC elasticity between samples from SCD patients treated with hydroxyurea and those that were not treated with hydroxyurea. Raman spectroscopy has been applied to both normal and pathological RBCs in previous studies, but the results were limited to the identification of characteristic signatures. Our integration of the two methods, Raman spectroscopy and laser tweezers, is a new biophotonic approach for studying the intricate relationship between the biochemical and mechanical properties of a single cell that was not previously possible and is not easily carried out with existing methods. As such, we believe that this recent emerging single cell-based technology can be developed and used as an RBC functional assay. We have previously shown that LTRS can detect a force dependent oxygenation state transition in individual RBCs trapped at different laser powers, presumably due to the optical forces stretching the cell and inducing changes in the hemoglobin-hemoglobin and hemoglobin-membrane interactions that change the oxygen content of the cell. We have also shown characteristic Raman signatures indicating different oxygen content in different RBC samples at specific applied forces. Signatures were different among RBCs from cord, normal adult, and SCD patient blood, suggesting laser tweezers Raman spectroscopy can be used to measure the different force dependent oxygen content in a single RBC. We are currently validating our system to determine whether Raman spectroscopy can be used to identify and distinguish gene-corrected RBCs from normal and sickle RBCs. Our preliminary results showed distinct Raman fingerprints associated with the membrane structure of RBCs. Spectral fingerprints were observed to be different between normal and sickle RBCs, and gene-corrected RBCs were observed to have Raman profiles similar to those of normal RBCs. We speculate that the signature overlap between normal and gene-corrected samples is due to partial gene correction. These results are encouraging and strongly suggest that Raman spectroscopy can not only identify molecular structures but also be used as a functional assay. Disclosures: No relevant conflicts of interest to declare.

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