scholarly journals Rotational dynamics of HIV-1 nucleocapsid protein NCp7 as probed by a spin label attached by peptide synthesis

Biopolymers ◽  
2008 ◽  
Vol 89 (12) ◽  
pp. 1125-1135 ◽  
Author(s):  
Zhiwen Zhang ◽  
Xiangmei Xi ◽  
Charles P. Scholes ◽  
Christine B. Karim
Keyword(s):  
Peptide Synthesis ◽  
Spin Label ◽  
Nucleocapsid Protein ◽  
Rotational Dynamics ◽  
Hiv 1

HIV-1 Nucleocapsid Protein NCp7 and Its RNA Stem Loop 3 Partner: Rotational Dynamics of Spin-Labeled RNA Stem Loop 3†

Biochemistry ◽  
2008 ◽  
Vol 47 (38) ◽  
pp. 10099-10110 ◽  
Author(s):  
Xiangmei Xi ◽  
Yan Sun ◽  
Christine B. Karim ◽  
Vladimir M. Grigoryants ◽  
Charles P. Scholes
Keyword(s):  
Nucleocapsid Protein ◽  
Rotational Dynamics ◽  
Stem Loop ◽  
Labeled Rna ◽  
Hiv 1

scholarly journals HIV-1 inactivation by 4-vinylpyridine is enhanced by dissociating Zn2+ from nucleocapsid protein

Virology ◽  
2008 ◽  
Vol 375 (1) ◽  
pp. 148-158 ◽  
Author(s):  
David R. Morcock ◽  
James A. Thomas ◽  
Raymond C. Sowder ◽  
Louis E. Henderson ◽  
Bruce J. Crise ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Hiv 1

Identification of HIV-1 nucleocapsid protein: nucleic acid antagonists with cellular anti-HIV activity

2002 ◽  
Vol 296 (5) ◽  
pp. 1228-1237 ◽  
Author(s):  
Andrew G Stephen ◽  
Karen M Worthy ◽  
Eric Towler ◽  
Judy A Mikovits ◽  
Shizuko Sei ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleocapsid Protein ◽  
Protein Nucleic Acid ◽  
Anti Hiv ◽  
Hiv 1

scholarly journals Synthesis and Evaluation of Bifunctional Aminothiazoles as Antiretrovirals Targeting the HIV-1 Nucleocapsid Protein

2018 ◽  
Vol 10 (4) ◽  
pp. 463-468 ◽  
Author(s):  
Mattia Mori ◽  
Maria Chiara Dasso Lang ◽  
Francesco Saladini ◽  
Nastasja Palombi ◽  
Lesia Kovalenko ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Hiv 1

Rationally Designed Peptides as Efficient Inhibitors of Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein

Biochemistry ◽  
2018 ◽  
Vol 57 (30) ◽  
pp. 4562-4573 ◽  
Author(s):  
Volodymyr Shvadchak ◽  
Sarwat Zgheib ◽  
Beata Basta ◽  
Nicolas Humbert ◽  
Johannes Langedijk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleocapsid Protein ◽  
Chaperone Activity ◽  
Nucleic Acid Chaperone ◽  
Hiv 1

scholarly journals Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Nuclear Localization Mediates Early Viral mRNA Expression

2002 ◽  
Vol 76 (20) ◽  
pp. 10444-10454 ◽  
Author(s):  
Jielin Zhang ◽  
Clyde S. Crumpacker
Keyword(s):  
Human Immunodeficiency Virus ◽  
Mrna Expression ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Viral Mrna ◽  
Genomic Rna ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT An important aspect of the pathophysiology of human immunodeficiency virus type 1 (HIV-1) infection is the ability of the virus to replicate in the host vigorously without a latent phase and to kill cells with a dynamic turnover of 1.8 × 109 cells/day and 10.3 × 109 virions/24 h. The transcription of HIV-1 RNA in acute infection occurs at two stages; the transcription of viral spliced mRNA occurs early, and the transcription of viral genomic RNA occurs later. The HIV-1 Tat protein is translated from the early spliced mRNA and is critical for HIV-1 genomic RNA expression. The cellular transcription factors are important for HIV-1 early spliced mRNA expression. In this study we show that virion nucleocapsid protein (NC) has a role in expression of HIV-1 early spliced mRNA. The HIV-1 NC migrates from the cytoplasm to the nucleus and accumulates in the nucleus at 18 h postinfection. Mutations on HIV-1 NC zinc fingers change the pattern of early viral spliced mRNA expression and result in a delayed expression of early viral mRNA in HIV-infected cells. This delayed HIV-1 early spliced mRNA expression occurs after proviral DNA has been integrated into the cellular genome, as shown by a quantitative integration assay. These results show that virion NC plays an important role in inducing HIV-1 early mRNA expression and contributes to the rapid viral replication that occurs during HIV-1 infection.

scholarly journals A Class of Potent Inhibitors of the HIV-1 Nucleocapsid Protein Based on Aminopyrrolic Scaffolds

2020 ◽  
Vol 11 (5) ◽  
pp. 698-705
Author(s):  
Stefano Ciaco ◽  
Nicolas Humbert ◽  
Eléonore Real ◽  
Christian Boudier ◽  
Oscar Francesconi ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Hiv 1

scholarly journals Reconciling NMR Structures of the HIV-1 Nucleocapsid Protein (NCp7) using Extensive Polarizable Force Field Free-Energy Simulations

2020 ◽  
Author(s):  
Léa El Khoury ◽  
Frédéric Célerse ◽  
Louis Lagardere ◽  
Luc-Henri Jolly ◽  
Étienne Derat ◽  
...  
Keyword(s):  
Free Energy ◽  
Nucleocapsid Protein ◽  
Extended Form ◽  
Polarizable Force Field ◽  
Relative Free Energy ◽  
Nmr Structures ◽  
Reference Protein ◽  
Energy Simulations ◽  
Hiv 1 ◽  
Most Probable Structure

Using polarizable (AMOEBA) and non-polarizable (CHARMM) force fields, we compare the relative free-energy stability of two extreme conformations of the HIV-1 NCp7 nucleocapsid that had been previously experimentally advocated to prevail in solution. Using accelerated sampling techniques, we show that they differ in stability by no more than 0.75-1.9 kcal/mol depending on the reference protein sequence. While the extended form appears to be the most probable structure, both forms should thus coexist in water explaining the differing NMR findings.<br>

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