Self-crosslinked and reducible fusogenic peptides for intracellular delivery of siRNA

Biopolymers ◽  
2008 ◽  
Vol 89 (10) ◽  
pp. 881-888 ◽  
Author(s):  
Hyejung Mok ◽  
Tae Gwan Park
Keyword(s):  
Intracellular Delivery ◽  
Fusogenic Peptides

Human-derived fusogenic peptides for the intracellular delivery of proteins

2017 ◽  
Vol 255 ◽  
pp. 1-11 ◽  
Author(s):  
Kei Sudo ◽  
Keisuke Niikura ◽  
Kouta Iwaki ◽  
Shunshi Kohyama ◽  
Kei Fujiwara ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Fusogenic Peptides

scholarly journals Biofunctional Peptide-Modified Extracellular Vesicles Enable Effective Intracellular Delivery via the Induction of Macropinocytosis

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 224
Author(s):  
Ikuhiko Nakase
Keyword(s):  
Extracellular Vesicles ◽  
Cellular Uptake ◽  
Leucine Zipper ◽  
Coiled Coil ◽  
Extracellular Fluid ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptides ◽  
Important Process ◽  
Actin Reorganization ◽  
Fusogenic Peptides

We previously reported that macropinocytosis (accompanied by actin reorganization, ruffling of the plasma membrane, and engulfment of large volumes of extracellular fluid) is an important process for the cellular uptake of extracellular vesicles, exosomes. Accordingly, we developed techniques to induce macropinocytosis by the modification of biofunctional peptides on exosomal membranes, thereby enhancing their cellular uptake. Arginine-rich cell-penetrating peptides have been shown to induce macropinocytosis via proteoglycans; accordingly, we developed peptide-modified exosomes that could actively induce macropinocytotic uptake by cells. In addition, the activation of EGFR induces macropinocytosis; based on this knowledge, we developed artificial leucine-zipper peptide (K4)-modified exosomes. These exosomes can recognize E3 sequence-fused EGFR (E3-EGFR), leading to the clustering and activation of E3-EGFR by coiled-coil formation (E3/K4), which induces cellular exosome uptake by macropinocytosis. In addition, modification of pH-sensitive fusogenic peptides (e.g., GALA) also enhances the cytosolic release of exosomal contents. These experimental techniques and findings using biofunctional peptides have contributed to the development of exosome-based intracellular delivery systems.

Probing Cellular Outcomes Using Heterobivalent Constructs

2019 ◽  
Author(s):  
Rohit Bhadoria ◽  
Kefeng Ping ◽  
Christer Lohk ◽  
Ivar Järving ◽  
Pavel Starkov
Keyword(s):  
Cell Viability ◽  
Small Molecules ◽  
Cellular Uptake ◽  
Kinase Inhibitors ◽  
Rho Kinase ◽  
Intracellular Delivery ◽  
Rho Kinase Inhibitors

<div> <div> <div> <p>Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes. </p> </div> </div> </div>

Intracellular Delivery of Nanocarriers for Cancer Therapy

2007 ◽  
Vol 3 (4) ◽  
pp. 329-338 ◽  
Author(s):  
Yah-el Har-el ◽  
Yoshinori Kato
Keyword(s):  
Cancer Therapy ◽  
Intracellular Delivery

DNA Dendrons as Agents for Intracellular Delivery

2021 ◽  
Author(s):  
Max E. Distler ◽  
Michelle H. Teplensky ◽  
Katherine E. Bujold ◽  
Caroline D. Kusmierz ◽  
Michael Evangelopoulos ◽  
...  
Keyword(s):  
Intracellular Delivery
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