PFG-NMR diffusometry: A tool for investigating the structure and dynamics of noncommercial purified pig gastric mucin in a wide range of concentrations

Biopolymers ◽  
2007 ◽  
Vol 86 (2) ◽  
pp. 165-175 ◽  
Author(s):  
G. Lafitte ◽  
O. Söderman ◽  
K. Thuresson ◽  
J. Davies
Keyword(s):  
Gastric Mucin ◽  
Structure And Dynamics ◽  
Wide Range ◽  
Pfg Nmr ◽  
Nmr Diffusometry

scholarly journals Quantification of the electrostatic and covalent interaction between whey proteins and low methoxyl pectin using PFG-NMR diffusometry

2019 ◽  
Vol 57 (9) ◽  
pp. 719-729 ◽  
Author(s):  
Arima Diah Setiowati ◽  
Lien Vermeir ◽  
Lorenz De Neve ◽  
Ali Sedaghat Doost ◽  
Davy Sinnaeve ◽  
...  
Keyword(s):  
Whey Proteins ◽  
Covalent Interaction ◽  
Pfg Nmr ◽  
Nmr Diffusometry

Monte Carlo simulation of chlorine adsorbed on graphite

1988 ◽  
Vol 66 (4) ◽  
pp. 955-962 ◽  
Author(s):  
Soong-Hyuck Suh ◽  
Seamus F. O'Shea
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Basal Plane ◽  
Thermodynamic Data ◽  
Molecular Chlorine ◽  
Monte Carlo Calculations ◽  
Structure And Dynamics ◽  
Wide Range ◽  
Do So ◽  
Molecule Surface

Monte Carlo calculations are reported for patches of molecular chlorine adsorbed on the basal plane of graphite. A variety of combinations of intermolecular and molecule–surface potentials have been tested, and the resulting structural predictions cover a wide range of behaviour. None of the combinations employed here can explain the recent experimental observations, but it is clear that at least some could be adjusted to do so. However, the lack of thermodynamic data concerning the interaction between the molecules and the surface presents a major obstacle. Further experiments which give information about the energetics of the system are needed before a definitive account of the structure and dynamics can be given.

scholarly journals Biological Activity of Porcine Gastric Mucin on Stress Resistance and Immunomodulation

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 2981 ◽  
Author(s):  
Thiloma D. Liyanage ◽  
Pasan S. Dahanayake ◽  
Shan L. Edirisinghe ◽  
Chamilani Nikapitiya ◽  
Gang-Joon Heo ◽  
...  
Keyword(s):  
Heat Tolerance ◽  
Survival Rates ◽  
Gastric Mucin ◽  
Raw 264.7 Cells ◽  
Hatching Rate ◽  
Raw 264.7 ◽  
Ros Scavenging ◽  
Antioxidant Genes ◽  
Porcine Gastric Mucin ◽  
Wide Range

Purified porcine gastric mucin (PGM) is an alternative biomaterial to native mucin which displays multifunctional properties for exploring a wide range of biomedical applications. The present study evaluated the in vitro (RAW 264.7 macrophage cells) and in vivo (zebrafish embryos and larvae) bioactivities of PGM. The median lethal concentration (LC50) of PGM was 197.9 µg/mL for embryos, while it was non-toxic to RAW 264.7 cells, even at 500 µg/mL. Following PGM exposure (100 µg/mL), a higher embryo hatching rate (59.9%) was observed at 48 h post fertilization, compared to the control (30.6%). Protective effects of PGM from pathogenic Aeromonas hydrophila were demonstrated by high larvae survival rates of 85.0% and 94.0% at 50 and 100 μg/mL of PGM exposure, respectively. Heat tolerance effect of PGM (50 and 100 µg/mL) on larvae (40 °C for 48 h) was confirmed by 75% and 100% of survival rates, respectively. Additionally, PGM reduced the A. hydrophila–induced reactive oxygen species (ROS) generation in larvae. The qRT-PCR results in PGM exposed larvae exhibited induction of immune-related genes (tlr5a and tlr5b, myd88, c-rel, il1β, tnf-α, il6, il10, cxcl18b, ccl34a.4, defbl1, hamp, ctsd, muc2.1, muc5.1, muc5.2, and muc5.3), stress response (hsp70, hsp90aa1.1, and hsp90ab1), and antioxidant genes (cat and sod1). Moreover, our results revealed that PGM involved in the regulation of transcriptional gene induction increases Hsp90 protein in the zebrafish larvae. Furthermore, upregulation of Il6, Il10, Tnfα, Ccl3, Defa-rs2, Defa21 and Camp and antioxidant genes (Sod2 and Cat) were observed in PGM-exposed RAW 264.7 cells. Overall findings confirmed the activation of immune responses, disease resistance against pathogenic bacteria, heat tolerance, and ROS-scavenging properties by PGM, which may provide insights into new applications for PGM as a multifunctional immunomodulator.

Transport enhancement in binderless zeolite X- and A-type molecular sieves revealed by PFG NMR diffusometry

2014 ◽  
Vol 188 ◽  
pp. 126-132 ◽  
Author(s):  
Dirk Mehlhorn ◽  
Rustem Valiullin ◽  
Jörg Kärger ◽  
Kristin Schumann ◽  
Alfons Brandt ◽  
...  
Keyword(s):  
Molecular Sieves ◽  
Zeolite X ◽  
Transport Enhancement ◽  
Pfg Nmr ◽  
Nmr Diffusometry

scholarly journals Teaching crystallography and material science focusing on large scale facilities

2014 ◽  
Vol 70 (a1) ◽  
pp. C1279-C1279
Author(s):  
Philippe Rabiller ◽  
Winfried Petry ◽  
Wolfgang Schmahl ◽  
Carlo Lamberti ◽  
Werner Paulus
Keyword(s):  
Material Science ◽  
Summer School ◽  
Large Scale ◽  
Free Electron Lasers ◽  
Master Program ◽  
Structure And Dynamics ◽  
Wide Range ◽  
Master Thesis ◽  
Master Level ◽  
Intensive Programs

Modern crystallography makes intense use of large scale facilities: neutron reactors, synchrotron sources, free electron lasers, where sources, optics and detectors allow for a wide range of possible experiments putting forwards the limits of the analysis of the structure and dynamics of matter and materials. Giving the large scale facilities a major role in the teaching of crystallography and material science, allowing for intense practice, requires to gather different skills which is more often done through summer school or intensive programs at PhD or junior scientist level. The Erasmus Mundus Master Course MaMaSELF (Master in Material Science Exploring Large Scale Facilities) is a unique European master program focused on the use of large scale facilities to investigate intimate nature of matter and materials where the five consortium higher education institutions (University of Rennes 1, France; Technische Universität München and Ludwig Maximilian University in München, Germany; University of Torino, Italy and University of Montpellier 2, France) have managed, together with Large Scale Facilities partners (ESRF, ILL, FRMII, DESY, LLB, SOLEIL, PSI) and third country partners spread out all over the world (Brazil, India, Japan, Russia, Switzerland , USA), to offer to the students a two years program at the master level including large amount of crystallography and spectroscopy teaching and an intensive summer-school totally dedicated to large scale facilities and including a large proportion of lessons and labs taught by experts, as well as many internship and master thesis opportunities at the large scale facilities.

scholarly journals Regulation of the Postsynaptic Compartment of Excitatory Synapses by the Actin Cytoskeleton in Health and Its Disruption in Disease

2016 ◽  
Vol 2016 ◽  
pp. 1-19 ◽  
Author(s):  
Holly Stefen ◽  
Chanchanok Chaichim ◽  
John Power ◽  
Thomas Fath
Keyword(s):  
Actin Cytoskeleton ◽  
Neurological Diseases ◽  
Synaptic Function ◽  
Excitatory Synapses ◽  
Key Factors ◽  
Complex Interplay ◽  
Structure And Dynamics ◽  
Wide Range ◽  
Associated Proteins ◽  
Cytoskeletal Elements

Disruption of synaptic function at excitatory synapses is one of the earliest pathological changes seen in wide range of neurological diseases. The proper control of the segregation of neurotransmitter receptors at these synapses is directly correlated with the intact regulation of the postsynaptic cytoskeleton. In this review, we are discussing key factors that regulate the structure and dynamics of the actin cytoskeleton, the major cytoskeletal building block that supports the postsynaptic compartment. Special attention is given to the complex interplay of actin-associated proteins that are found in the synaptic specialization. We then discuss our current understanding of how disruption of these cytoskeletal elements may contribute to the pathological events observed in the nervous system under disease conditions with a particular focus on Alzheimer’s disease pathology.

Ethene/ethane mixture diffusion in the MOF sieve ZIF-8 studied by MAS PFG NMR diffusometry

2012 ◽  
Vol 147 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Christian Chmelik ◽  
Dieter Freude ◽  
Helge Bux ◽  
Jürgen Haase
Keyword(s):  
Pfg Nmr ◽  
Nmr Diffusometry

scholarly journals New Strategic Approaches to Gaining from Emerging Advanced Manufacturing Markets

2021 ◽  
Vol 15 (3) ◽  
pp. 6-21
Author(s):  
Yuri Simachev ◽  
◽  
Anna Fedyunina ◽  
Maksim Yurevich ◽  
Mikhail Kuzyk ◽  
...  
Keyword(s):  
Industrial Policy ◽  
Industry 4.0 ◽  
High Tech ◽  
Advanced Manufacturing ◽  
Industrial Policies ◽  
Competitive Edge ◽  
Structure And Dynamics ◽  
Wide Range ◽  
Current Context

Advanced Manufacturing (AM) markets are a major factor of contemporary worldwide growth that to a large extent determines countries’ competitiveness. Strengthening and/or optimizing the positions on AM markets is among the major challenges for modern industrial policy. This article discusses the structure and dynamics of the development of advanced manufacturing markets, as well as the specifics of the policies of the countries strengthening their positions in these markets. Gaining entry into AM markets currently implies individual countries’ and industries’ adopting different models which combine a wide range of factors. Small nations are rapidly applying such approaches, gaining advantages and thus increasing their competitive edge, which creates certain challenges for leading high-tech countries too slow to adjust their industrial policies. So far the basis for Industry 4.0 markets is just emerging, and remains limited to a few nations including developing ones. Country cases are presented below to illustrate the development of AM markets. The authors conclude that in the current context, no universal approaches to shaping a successful industrial policy remain. The most productive strategy is to combine the unique advantages of a particular economy.

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